Experiment: ALP with Fusidic 0.02 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Dsui_2665 and Dsui_2666 are separated by 144 nucleotides Dsui_2666 and Dsui_2667 are separated by 12 nucleotides Dsui_2667 and Dsui_2668 overlap by 4 nucleotides
Dsui_2665: Dsui_2665 - putative nucleoside-diphosphate sugar epimerase, at 2,817,459 to 2,818,313
_2665
Dsui_2666: Dsui_2666 - putative transcriptional regulator, at 2,818,458 to 2,818,901
_2666
Dsui_2667: Dsui_2667 - acetyltransferase, at 2,818,914 to 2,819,447
_2667
Dsui_2668: Dsui_2668 - putative phosphatase, at 2,819,444 to 2,821,390
_2668
Position (kb)
2818
2819 Strain fitness (log2 ratio)
-2
-1
0
1 at 2817.519 kb on + strand at 2817.655 kb on - strand at 2817.672 kb on - strand at 2817.708 kb on + strand at 2817.716 kb on - strand at 2817.868 kb on + strand at 2817.882 kb on + strand at 2817.922 kb on + strand at 2817.923 kb on + strand, within Dsui_2665 at 2818.005 kb on + strand at 2818.013 kb on - strand at 2818.059 kb on - strand at 2818.093 kb on + strand at 2818.357 kb on - strand at 2818.378 kb on + strand at 2818.392 kb on - strand at 2818.447 kb on + strand at 2818.455 kb on + strand at 2818.471 kb on - strand at 2818.569 kb on - strand, within Dsui_2666 at 2818.597 kb on - strand, within Dsui_2666 at 2818.693 kb on - strand, within Dsui_2666 at 2818.754 kb on - strand, within Dsui_2666 at 2818.758 kb on + strand, within Dsui_2666 at 2819.006 kb on - strand at 2819.118 kb on + strand, within Dsui_2667 at 2819.274 kb on + strand, within Dsui_2667 at 2819.274 kb on + strand, within Dsui_2667 at 2819.389 kb on + strand, within Dsui_2667 at 2819.407 kb on + strand at 2819.455 kb on - strand at 2819.617 kb on + strand at 2819.662 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction ALP with Fusidic 0.02 mg/ml remove 2,817,519 + -0.2 2,817,655 - -0.8 2,817,672 - +0.8 2,817,708 + +0.8 2,817,716 - +0.8 2,817,868 + +0.8 2,817,882 + -0.2 2,817,922 + -0.2 2,817,923 + Dsui_2665 0.54 -1.2 2,818,005 + +0.8 2,818,013 - -0.2 2,818,059 - +0.8 2,818,093 + +0.8 2,818,357 - +0.8 2,818,378 + -0.2 2,818,392 - -1.2 2,818,447 + -0.5 2,818,455 + -0.4 2,818,471 - -0.2 2,818,569 - Dsui_2666 0.25 -0.2 2,818,597 - Dsui_2666 0.31 -0.7 2,818,693 - Dsui_2666 0.53 -2.2 2,818,754 - Dsui_2666 0.67 -0.2 2,818,758 + Dsui_2666 0.68 -0.7 2,819,006 - -1.2 2,819,118 + Dsui_2667 0.38 +0.8 2,819,274 + Dsui_2667 0.67 -2.2 2,819,274 + Dsui_2667 0.67 +0.8 2,819,389 + Dsui_2667 0.89 -0.8 2,819,407 + +0.8 2,819,455 - -0.8 2,819,617 + +0.8 2,819,662 + +0.8
Or see this region's nucleotide sequence