Experiment: ALP with Fusidic 0.02 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Dsui_2636 and Dsui_2637 overlap by 4 nucleotides Dsui_2637 and Dsui_2638 are separated by 80 nucleotides Dsui_2638 and Dsui_2639 are separated by 21 nucleotides Dsui_2639 and Dsui_2640 are separated by 96 nucleotides
Dsui_2636: Dsui_2636 - RND family efflux transporter, MFP subunit, at 2,788,227 to 2,789,816
_2636
Dsui_2637: Dsui_2637 - outer membrane protein, at 2,789,813 to 2,791,084
_2637
Dsui_2638: Dsui_2638 - hypothetical protein, at 2,791,165 to 2,791,509
_2638
Dsui_2639: Dsui_2639 - putative metal-binding protein, at 2,791,531 to 2,791,971
_2639
Dsui_2640: Dsui_2640 - Cytochrome c, at 2,792,068 to 2,792,730
_2640
Position (kb)
2789
2790
2791
2792 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 2788.936 kb on - strand, within Dsui_2636 at 2788.962 kb on + strand, within Dsui_2636 at 2788.963 kb on + strand, within Dsui_2636 at 2788.971 kb on - strand, within Dsui_2636 at 2788.971 kb on - strand, within Dsui_2636 at 2788.971 kb on - strand, within Dsui_2636 at 2788.971 kb on - strand, within Dsui_2636 at 2788.971 kb on - strand, within Dsui_2636 at 2788.983 kb on - strand, within Dsui_2636 at 2789.152 kb on + strand, within Dsui_2636 at 2789.160 kb on - strand, within Dsui_2636 at 2789.160 kb on - strand, within Dsui_2636 at 2789.314 kb on - strand, within Dsui_2636 at 2789.336 kb on + strand, within Dsui_2636 at 2789.494 kb on - strand, within Dsui_2636 at 2789.569 kb on + strand, within Dsui_2636 at 2789.571 kb on + strand, within Dsui_2636 at 2789.577 kb on - strand, within Dsui_2636 at 2789.579 kb on - strand, within Dsui_2636 at 2789.634 kb on - strand, within Dsui_2636 at 2789.639 kb on - strand, within Dsui_2636 at 2789.798 kb on + strand at 2789.801 kb on - strand at 2789.806 kb on + strand at 2789.806 kb on - strand at 2789.996 kb on - strand, within Dsui_2637 at 2789.996 kb on - strand, within Dsui_2637 at 2789.996 kb on - strand, within Dsui_2637 at 2790.119 kb on - strand, within Dsui_2637 at 2790.166 kb on - strand, within Dsui_2637 at 2790.199 kb on + strand, within Dsui_2637 at 2790.220 kb on + strand at 2790.225 kb on + strand, within Dsui_2637 at 2790.228 kb on - strand, within Dsui_2637 at 2790.228 kb on - strand, within Dsui_2637 at 2790.228 kb on - strand, within Dsui_2637 at 2790.327 kb on - strand, within Dsui_2637 at 2790.327 kb on - strand, within Dsui_2637 at 2790.327 kb on - strand, within Dsui_2637 at 2790.365 kb on - strand, within Dsui_2637 at 2790.365 kb on - strand, within Dsui_2637 at 2790.519 kb on - strand, within Dsui_2637 at 2790.647 kb on - strand, within Dsui_2637 at 2790.695 kb on - strand, within Dsui_2637 at 2790.697 kb on + strand, within Dsui_2637 at 2790.705 kb on - strand, within Dsui_2637 at 2790.946 kb on + strand, within Dsui_2637 at 2790.946 kb on + strand, within Dsui_2637 at 2790.977 kb on - strand at 2790.987 kb on - strand at 2790.991 kb on - strand at 2791.015 kb on - strand at 2791.135 kb on + strand at 2791.143 kb on + strand at 2791.151 kb on - strand at 2791.210 kb on - strand, within Dsui_2638 at 2791.306 kb on + strand, within Dsui_2638 at 2791.369 kb on - strand, within Dsui_2638 at 2791.369 kb on - strand, within Dsui_2638 at 2791.439 kb on + strand, within Dsui_2638 at 2791.460 kb on + strand, within Dsui_2638 at 2791.503 kb on - strand at 2791.546 kb on + strand at 2791.564 kb on + strand at 2791.604 kb on - strand, within Dsui_2639 at 2791.662 kb on - strand, within Dsui_2639 at 2791.728 kb on + strand, within Dsui_2639 at 2791.773 kb on - strand, within Dsui_2639 at 2791.801 kb on - strand, within Dsui_2639 at 2791.884 kb on - strand, within Dsui_2639 at 2791.928 kb on - strand at 2791.958 kb on - strand at 2791.998 kb on - strand at 2792.011 kb on - strand at 2792.065 kb on + strand at 2792.065 kb on + strand at 2792.073 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction ALP with Fusidic 0.02 mg/ml remove 2,788,936 - Dsui_2636 0.45 -0.2 2,788,962 + Dsui_2636 0.46 +0.8 2,788,963 + Dsui_2636 0.46 +0.8 2,788,971 - Dsui_2636 0.47 -0.8 2,788,971 - Dsui_2636 0.47 -0.2 2,788,971 - Dsui_2636 0.47 -1.5 2,788,971 - Dsui_2636 0.47 -0.8 2,788,971 - Dsui_2636 0.47 -0.2 2,788,983 - Dsui_2636 0.48 -1.8 2,789,152 + Dsui_2636 0.58 -0.2 2,789,160 - Dsui_2636 0.59 -0.8 2,789,160 - Dsui_2636 0.59 +0.8 2,789,314 - Dsui_2636 0.68 -2.1 2,789,336 + Dsui_2636 0.70 +0.8 2,789,494 - Dsui_2636 0.80 -0.2 2,789,569 + Dsui_2636 0.84 +0.8 2,789,571 + Dsui_2636 0.85 +0.8 2,789,577 - Dsui_2636 0.85 -2.2 2,789,579 - Dsui_2636 0.85 +0.8 2,789,634 - Dsui_2636 0.88 -1.2 2,789,639 - Dsui_2636 0.89 +0.8 2,789,798 + +0.8 2,789,801 - -0.2 2,789,806 + -0.8 2,789,806 - -1.2 2,789,996 - Dsui_2637 0.14 +0.8 2,789,996 - Dsui_2637 0.14 +0.8 2,789,996 - Dsui_2637 0.14 -1.5 2,790,119 - Dsui_2637 0.24 -0.2 2,790,166 - Dsui_2637 0.28 -0.2 2,790,199 + Dsui_2637 0.30 -2.2 2,790,220 + +0.8 2,790,225 + Dsui_2637 0.32 -0.2 2,790,228 - Dsui_2637 0.33 -1.5 2,790,228 - Dsui_2637 0.33 -0.2 2,790,228 - Dsui_2637 0.33 -0.8 2,790,327 - Dsui_2637 0.40 -0.2 2,790,327 - Dsui_2637 0.40 -0.2 2,790,327 - Dsui_2637 0.40 +2.2 2,790,365 - Dsui_2637 0.43 +0.8 2,790,365 - Dsui_2637 0.43 +0.8 2,790,519 - Dsui_2637 0.56 -0.8 2,790,647 - Dsui_2637 0.66 +0.8 2,790,695 - Dsui_2637 0.69 -0.8 2,790,697 + Dsui_2637 0.69 -1.2 2,790,705 - Dsui_2637 0.70 -1.5 2,790,946 + Dsui_2637 0.89 -1.2 2,790,946 + Dsui_2637 0.89 -0.8 2,790,977 - +0.8 2,790,987 - +0.8 2,790,991 - -0.8 2,791,015 - -0.2 2,791,135 + +0.8 2,791,143 + -0.2 2,791,151 - +0.8 2,791,210 - Dsui_2638 0.13 +0.8 2,791,306 + Dsui_2638 0.41 -0.2 2,791,369 - Dsui_2638 0.59 -0.8 2,791,369 - Dsui_2638 0.59 -0.8 2,791,439 + Dsui_2638 0.79 -0.2 2,791,460 + Dsui_2638 0.86 -0.2 2,791,503 - -3.0 2,791,546 + -1.2 2,791,564 + -0.2 2,791,604 - Dsui_2639 0.17 -0.8 2,791,662 - Dsui_2639 0.30 +0.8 2,791,728 + Dsui_2639 0.45 -0.8 2,791,773 - Dsui_2639 0.55 +0.8 2,791,801 - Dsui_2639 0.61 +0.8 2,791,884 - Dsui_2639 0.80 +0.8 2,791,928 - -0.2 2,791,958 - +0.8 2,791,998 - -0.8 2,792,011 - +0.8 2,792,065 + +0.8 2,792,065 + -0.2 2,792,073 - +0.8
Or see this region's nucleotide sequence