Experiment: ALP with Fusidic 0.02 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Dsui_2269 and Dsui_2270 are separated by 280 nucleotides Dsui_2270 and Dsui_2271 are separated by 54 nucleotides Dsui_2271 and Dsui_2272 are separated by 215 nucleotides
Dsui_2269: Dsui_2269 - SOS regulatory protein LexA, at 2,420,598 to 2,421,209
_2269
Dsui_2270: Dsui_2270 - hypothetical protein, at 2,421,490 to 2,421,693
_2270
Dsui_2271: Dsui_2271 - hypothetical protein, at 2,421,748 to 2,422,731
_2271
Dsui_2272: Dsui_2272 - hypothetical protein, at 2,422,947 to 2,424,431
_2272
Position (kb)
2421
2422
2423 Strain fitness (log2 ratio)
-1
0
1 at 2420.959 kb on - strand, within Dsui_2269 at 2421.207 kb on - strand at 2421.440 kb on + strand at 2421.487 kb on + strand at 2421.605 kb on - strand, within Dsui_2270 at 2421.618 kb on - strand at 2421.902 kb on - strand, within Dsui_2271 at 2422.011 kb on + strand at 2422.050 kb on + strand, within Dsui_2271 at 2422.066 kb on - strand, within Dsui_2271 at 2422.133 kb on - strand, within Dsui_2271 at 2422.287 kb on + strand, within Dsui_2271 at 2422.302 kb on + strand, within Dsui_2271 at 2422.310 kb on - strand, within Dsui_2271 at 2422.339 kb on + strand at 2422.417 kb on + strand at 2422.453 kb on - strand, within Dsui_2271 at 2422.559 kb on - strand, within Dsui_2271 at 2422.574 kb on + strand at 2422.611 kb on + strand at 2422.637 kb on + strand at 2422.730 kb on - strand at 2422.741 kb on + strand at 2422.776 kb on + strand at 2422.950 kb on + strand at 2423.020 kb on - strand at 2423.159 kb on + strand at 2423.167 kb on - strand, within Dsui_2272 at 2423.173 kb on - strand at 2423.244 kb on - strand, within Dsui_2272 at 2423.263 kb on - strand, within Dsui_2272 at 2423.279 kb on - strand, within Dsui_2272 at 2423.561 kb on + strand at 2423.651 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction ALP with Fusidic 0.02 mg/ml remove 2,420,959 - Dsui_2269 0.59 -0.6 2,421,207 - +0.3 2,421,440 + +0.9 2,421,487 + -0.1 2,421,605 - Dsui_2270 0.56 -0.7 2,421,618 - +0.9 2,421,902 - Dsui_2271 0.16 +0.8 2,422,011 + +0.9 2,422,050 + Dsui_2271 0.31 -0.1 2,422,066 - Dsui_2271 0.32 -0.1 2,422,133 - Dsui_2271 0.39 -0.6 2,422,287 + Dsui_2271 0.55 -0.1 2,422,302 + Dsui_2271 0.56 -0.7 2,422,310 - Dsui_2271 0.57 +0.6 2,422,339 + +0.9 2,422,417 + -0.1 2,422,453 - Dsui_2271 0.72 +0.8 2,422,559 - Dsui_2271 0.82 +0.4 2,422,574 + +0.9 2,422,611 + +0.9 2,422,637 + -0.7 2,422,730 - +0.1 2,422,741 + -0.1 2,422,776 + +0.9 2,422,950 + -1.1 2,423,020 - -0.1 2,423,159 + +0.9 2,423,167 - Dsui_2272 0.15 +0.9 2,423,173 - -0.1 2,423,244 - Dsui_2272 0.20 +0.9 2,423,263 - Dsui_2272 0.21 +0.9 2,423,279 - Dsui_2272 0.22 -0.1 2,423,561 + -0.7 2,423,651 + -0.1
Or see this region's nucleotide sequence