Experiment: ALP with Fusidic 0.02 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Dsui_2266 and Dsui_2267 are separated by 25 nucleotides Dsui_2267 and Dsui_2268 are separated by 466 nucleotides Dsui_2268 and Dsui_2269 are separated by 45 nucleotides
Dsui_2266: Dsui_2266 - nucleotidyltransferase/DNA polymerase involved in DNA repair, at 2,417,395 to 2,418,888
_2266
Dsui_2267: Dsui_2267 - hypothetical protein, at 2,418,914 to 2,419,648
_2267
Dsui_2268: Dsui_2268 - putative lactoylglutathione lyase, at 2,420,115 to 2,420,552
_2268
Dsui_2269: Dsui_2269 - SOS regulatory protein LexA, at 2,420,598 to 2,421,209
_2269
Position (kb)
2418
2419
2420 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 2417.939 kb on - strand, within Dsui_2266 at 2418.218 kb on - strand at 2418.222 kb on + strand at 2418.224 kb on - strand, within Dsui_2266 at 2418.342 kb on - strand at 2418.348 kb on - strand at 2418.413 kb on - strand at 2418.619 kb on - strand at 2418.719 kb on - strand, within Dsui_2266 at 2419.517 kb on + strand at 2419.522 kb on + strand at 2419.620 kb on - strand at 2419.659 kb on + strand at 2419.850 kb on - strand at 2419.855 kb on + strand at 2419.863 kb on - strand at 2419.972 kb on - strand at 2419.979 kb on - strand at 2420.007 kb on - strand at 2420.177 kb on + strand at 2420.234 kb on - strand, within Dsui_2268 at 2420.269 kb on + strand at 2420.269 kb on + strand at 2420.269 kb on + strand at 2420.277 kb on - strand, within Dsui_2268 at 2420.277 kb on - strand, within Dsui_2268 at 2420.342 kb on + strand at 2420.411 kb on + strand at 2420.424 kb on - strand, within Dsui_2268 at 2420.469 kb on + strand at 2420.530 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction ALP with Fusidic 0.02 mg/ml remove 2,417,939 - Dsui_2266 0.36 -2.1 2,418,218 - -0.1 2,418,222 + +0.9 2,418,224 - Dsui_2266 0.55 -0.1 2,418,342 - -1.4 2,418,348 - +0.9 2,418,413 - +0.9 2,418,619 - +0.9 2,418,719 - Dsui_2266 0.89 -1.7 2,419,517 + -0.1 2,419,522 + +0.9 2,419,620 - -0.1 2,419,659 + -0.1 2,419,850 - +0.9 2,419,855 + +0.9 2,419,863 - -0.1 2,419,972 - -1.1 2,419,979 - +0.9 2,420,007 - -0.7 2,420,177 + +0.9 2,420,234 - Dsui_2268 0.27 +2.4 2,420,269 + +0.9 2,420,269 + +0.9 2,420,269 + -0.1 2,420,277 - Dsui_2268 0.37 -0.4 2,420,277 - Dsui_2268 0.37 -0.7 2,420,342 + +0.9 2,420,411 + +0.9 2,420,424 - Dsui_2268 0.71 +0.3 2,420,469 + -0.1 2,420,530 - +0.4
Or see this region's nucleotide sequence