Experiment: ALP with Fusidic 0.02 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Dsui_2219 and Dsui_2220 are separated by 199 nucleotides Dsui_2220 and Dsui_2221 are separated by 271 nucleotides Dsui_2221 and Dsui_2222 are separated by 68 nucleotides
Dsui_2219: Dsui_2219 - dihydrolipoamide dehydrogenase, at 2,359,377 to 2,360,801
_2219
Dsui_2220: Dsui_2220 - hypothetical protein, at 2,361,001 to 2,361,321
_2220
Dsui_2221: Dsui_2221 - putative ATPase, at 2,361,593 to 2,362,708
_2221
Dsui_2222: Dsui_2222 - type II secretory pathway, component PulD, at 2,362,777 to 2,363,682
_2222
Position (kb)
2361
2362
2363 Strain fitness (log2 ratio)
-1
0
1
2
3 at 2360.795 kb on + strand at 2360.795 kb on + strand at 2360.843 kb on + strand at 2360.946 kb on + strand at 2360.951 kb on + strand at 2360.959 kb on - strand at 2360.973 kb on + strand at 2361.024 kb on - strand at 2361.028 kb on - strand at 2361.092 kb on + strand, within Dsui_2220 at 2361.136 kb on + strand at 2361.141 kb on - strand, within Dsui_2220 at 2361.180 kb on + strand at 2361.180 kb on - strand, within Dsui_2220 at 2361.188 kb on - strand, within Dsui_2220 at 2361.279 kb on - strand, within Dsui_2220 at 2361.377 kb on + strand at 2361.476 kb on + strand at 2361.479 kb on - strand at 2361.484 kb on - strand at 2361.484 kb on - strand at 2361.491 kb on + strand at 2361.538 kb on - strand at 2361.565 kb on + strand at 2361.608 kb on - strand at 2361.680 kb on - strand at 2361.833 kb on - strand at 2361.917 kb on + strand, within Dsui_2221 at 2362.164 kb on + strand, within Dsui_2221 at 2362.233 kb on + strand, within Dsui_2221 at 2362.233 kb on + strand, within Dsui_2221 at 2362.302 kb on + strand, within Dsui_2221 at 2362.325 kb on - strand at 2362.475 kb on + strand, within Dsui_2221 at 2362.604 kb on + strand at 2362.606 kb on + strand at 2362.606 kb on + strand at 2362.614 kb on - strand at 2362.614 kb on - strand at 2362.774 kb on + strand at 2362.808 kb on + strand at 2362.818 kb on + strand at 2362.847 kb on - strand at 2362.851 kb on + strand at 2362.985 kb on + strand, within Dsui_2222 at 2363.048 kb on + strand, within Dsui_2222 at 2363.058 kb on + strand, within Dsui_2222 at 2363.073 kb on - strand, within Dsui_2222 at 2363.222 kb on + strand, within Dsui_2222 at 2363.222 kb on + strand, within Dsui_2222 at 2363.361 kb on + strand, within Dsui_2222 at 2363.420 kb on - strand, within Dsui_2222 at 2363.426 kb on + strand, within Dsui_2222 at 2363.473 kb on + strand, within Dsui_2222 at 2363.513 kb on + strand, within Dsui_2222
Per-strain Table
Position Strand Gene LocusTag Fraction ALP with Fusidic 0.02 mg/ml remove 2,360,795 + +0.4 2,360,795 + +0.5 2,360,843 + +0.1 2,360,946 + -0.0 2,360,951 + -0.0 2,360,959 - -0.0 2,360,973 + -0.0 2,361,024 - +1.0 2,361,028 - +0.2 2,361,092 + Dsui_2220 0.28 -0.6 2,361,136 + +1.0 2,361,141 - Dsui_2220 0.44 +1.0 2,361,180 + +1.0 2,361,180 - Dsui_2220 0.56 -1.0 2,361,188 - Dsui_2220 0.58 -1.0 2,361,279 - Dsui_2220 0.87 +1.0 2,361,377 + +1.0 2,361,476 + +1.0 2,361,479 - -0.0 2,361,484 - -0.0 2,361,484 - -1.0 2,361,491 + +1.0 2,361,538 - -0.6 2,361,565 + +1.0 2,361,608 - +1.0 2,361,680 - -0.6 2,361,833 - +0.9 2,361,917 + Dsui_2221 0.29 +3.4 2,362,164 + Dsui_2221 0.51 +2.1 2,362,233 + Dsui_2221 0.57 +2.9 2,362,233 + Dsui_2221 0.57 +2.9 2,362,302 + Dsui_2221 0.64 +2.9 2,362,325 - +0.9 2,362,475 + Dsui_2221 0.79 +3.1 2,362,604 + +2.9 2,362,606 + +1.5 2,362,606 + +2.5 2,362,614 - +0.9 2,362,614 - +0.9 2,362,774 + -0.2 2,362,808 + +0.7 2,362,818 + +0.0 2,362,847 - +0.9 2,362,851 + +0.2 2,362,985 + Dsui_2222 0.23 +0.9 2,363,048 + Dsui_2222 0.30 +0.2 2,363,058 + Dsui_2222 0.31 +0.2 2,363,073 - Dsui_2222 0.33 -0.7 2,363,222 + Dsui_2222 0.49 -0.3 2,363,222 + Dsui_2222 0.49 +0.0 2,363,361 + Dsui_2222 0.64 +1.0 2,363,420 - Dsui_2222 0.71 +0.9 2,363,426 + Dsui_2222 0.72 +0.0 2,363,473 + Dsui_2222 0.77 +0.3 2,363,513 + Dsui_2222 0.81 +0.4
Or see this region's nucleotide sequence