Experiment: ALP with Fusidic 0.02 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Dsui_2209 and Dsui_2210 are separated by 188 nucleotides Dsui_2210 and Dsui_2211 are separated by 130 nucleotides Dsui_2211 and Dsui_2212 overlap by 7 nucleotides Dsui_2212 and Dsui_2213 are separated by 3 nucleotides
Dsui_2209: Dsui_2209 - malate dehydrogenase, at 2,348,177 to 2,349,163
_2209
Dsui_2210: Dsui_2210 - transcriptional regulator, at 2,349,352 to 2,350,092
_2210
Dsui_2211: Dsui_2211 - succinate dehydrogenase, cytochrome b556 subunit, at 2,350,223 to 2,350,615
_2211
Dsui_2212: Dsui_2212 - succinate dehydrogenase, hydrophobic membrane anchor protein, at 2,350,609 to 2,350,956
_2212
Dsui_2213: Dsui_2213 - succinate dehydrogenase, flavoprotein subunit, at 2,350,960 to 2,352,753
_2213
Position (kb)
2349
2350
2351 Strain fitness (log2 ratio)
-1
0
1 at 2349.289 kb on - strand at 2349.338 kb on + strand at 2349.338 kb on + strand at 2349.348 kb on + strand at 2349.368 kb on + strand at 2349.375 kb on + strand at 2349.383 kb on + strand at 2349.428 kb on + strand, within Dsui_2210 at 2349.439 kb on + strand, within Dsui_2210 at 2349.506 kb on + strand, within Dsui_2210 at 2349.589 kb on + strand, within Dsui_2210 at 2349.702 kb on + strand, within Dsui_2210 at 2349.703 kb on + strand, within Dsui_2210 at 2349.862 kb on + strand, within Dsui_2210 at 2349.977 kb on + strand, within Dsui_2210 at 2349.987 kb on + strand, within Dsui_2210 at 2350.081 kb on + strand at 2350.088 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction ALP with Fusidic 0.02 mg/ml remove 2,349,289 - -0.0 2,349,338 + +1.0 2,349,338 + +0.3 2,349,348 + +0.1 2,349,368 + -0.2 2,349,375 + -0.3 2,349,383 + +0.6 2,349,428 + Dsui_2210 0.10 +0.4 2,349,439 + Dsui_2210 0.12 -0.1 2,349,506 + Dsui_2210 0.21 +0.5 2,349,589 + Dsui_2210 0.32 -0.7 2,349,702 + Dsui_2210 0.47 -0.3 2,349,703 + Dsui_2210 0.47 -0.6 2,349,862 + Dsui_2210 0.69 -1.4 2,349,977 + Dsui_2210 0.84 -0.5 2,349,987 + Dsui_2210 0.86 -1.6 2,350,081 + -1.0 2,350,088 + +0.2
Or see this region's nucleotide sequence