Experiment: ALP with Fusidic 0.02 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Dsui_2136 and Dsui_2137 are separated by 132 nucleotides Dsui_2137 and Dsui_2138 are separated by 35 nucleotides Dsui_2138 and Dsui_2139 are separated by 136 nucleotides
Dsui_2136: Dsui_2136 - Fe-S oxidoreductase, at 2,265,531 to 2,266,382
_2136
Dsui_2137: Dsui_2137 - queuosine biosynthesis protein QueD, at 2,266,515 to 2,266,961
_2137
Dsui_2138: Dsui_2138 - putative esterase, at 2,266,997 to 2,267,665
_2138
Dsui_2139: Dsui_2139 - Protein of unknown function (DUF3348), at 2,267,802 to 2,268,530
_2139
Position (kb)
2266
2267 Strain fitness (log2 ratio)
-2
-1
0
1 at 2266.422 kb on - strand at 2266.515 kb on + strand at 2266.518 kb on + strand at 2266.715 kb on + strand, within Dsui_2137 at 2266.790 kb on + strand, within Dsui_2137 at 2266.808 kb on + strand, within Dsui_2137 at 2266.960 kb on + strand at 2266.987 kb on + strand at 2267.040 kb on + strand at 2267.045 kb on + strand at 2267.073 kb on - strand at 2267.118 kb on + strand, within Dsui_2138 at 2267.232 kb on + strand, within Dsui_2138 at 2267.497 kb on + strand, within Dsui_2138 at 2267.610 kb on + strand at 2267.707 kb on - strand at 2267.744 kb on + strand at 2267.745 kb on + strand at 2267.799 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction ALP with Fusidic 0.02 mg/ml remove 2,266,422 - +0.2 2,266,515 + -0.0 2,266,518 + +0.1 2,266,715 + Dsui_2137 0.45 +0.9 2,266,790 + Dsui_2137 0.62 +0.7 2,266,808 + Dsui_2137 0.66 +1.3 2,266,960 + +0.1 2,266,987 + +0.8 2,267,040 + -0.0 2,267,045 + -0.3 2,267,073 - +0.1 2,267,118 + Dsui_2138 0.18 -0.1 2,267,232 + Dsui_2138 0.35 -2.0 2,267,497 + Dsui_2138 0.75 -0.2 2,267,610 + -0.0 2,267,707 - +0.1 2,267,744 + +0.8 2,267,745 + +0.2 2,267,799 - +1.0
Or see this region's nucleotide sequence