Experiment: ALP with Fusidic 0.02 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Dsui_2125 and Dsui_2126 are separated by 197 nucleotides Dsui_2126 and Dsui_2127 are separated by 67 nucleotides Dsui_2127 and Dsui_2128 are separated by 143 nucleotides
Dsui_2125: Dsui_2125 - PAS domain S-box, at 2,251,301 to 2,254,285
_2125
Dsui_2126: Dsui_2126 - alanine dehydrogenase, at 2,254,483 to 2,255,574
_2126
Dsui_2127: Dsui_2127 - flavodoxin reductase family protein, at 2,255,642 to 2,256,430
_2127
Dsui_2128: Dsui_2128 - uncharacterized protein involved in outer membrane biogenesis, at 2,256,574 to 2,259,795
_2128
Position (kb)
2254
2255
2256 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 2253.560 kb on + strand at 2253.601 kb on - strand, within Dsui_2125 at 2253.682 kb on - strand, within Dsui_2125 at 2253.690 kb on + strand at 2253.737 kb on - strand at 2253.869 kb on - strand, within Dsui_2125 at 2253.869 kb on - strand at 2254.177 kb on + strand at 2254.177 kb on + strand at 2254.222 kb on + strand at 2254.230 kb on - strand at 2254.235 kb on + strand at 2254.336 kb on + strand at 2254.342 kb on - strand at 2254.393 kb on - strand at 2254.422 kb on + strand at 2254.444 kb on + strand at 2254.553 kb on - strand at 2254.675 kb on - strand, within Dsui_2126 at 2254.770 kb on - strand, within Dsui_2126 at 2255.248 kb on - strand, within Dsui_2126 at 2255.255 kb on + strand at 2255.517 kb on + strand at 2255.847 kb on - strand at 2255.990 kb on - strand at 2256.090 kb on - strand, within Dsui_2127 at 2256.123 kb on - strand at 2256.128 kb on - strand at 2256.128 kb on - strand, within Dsui_2127 at 2256.172 kb on + strand, within Dsui_2127 at 2256.440 kb on + strand at 2256.440 kb on + strand at 2256.448 kb on - strand at 2256.448 kb on - strand at 2256.491 kb on + strand at 2256.499 kb on - strand at 2256.571 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction ALP with Fusidic 0.02 mg/ml remove 2,253,560 + +1.1 2,253,601 - Dsui_2125 0.77 -2.3 2,253,682 - Dsui_2125 0.80 -0.8 2,253,690 + +1.1 2,253,737 - +1.1 2,253,869 - Dsui_2125 0.86 +1.1 2,253,869 - +0.1 2,254,177 + +1.1 2,254,177 + +1.1 2,254,222 + +0.1 2,254,230 - +1.1 2,254,235 + +0.1 2,254,336 + +0.1 2,254,342 - +0.1 2,254,393 - +0.1 2,254,422 + -0.5 2,254,444 + +1.1 2,254,553 - +0.1 2,254,675 - Dsui_2126 0.18 +0.1 2,254,770 - Dsui_2126 0.26 -0.9 2,255,248 - Dsui_2126 0.70 +1.1 2,255,255 + +1.1 2,255,517 + -1.5 2,255,847 - +0.1 2,255,990 - +1.1 2,256,090 - Dsui_2127 0.57 -0.5 2,256,123 - +1.1 2,256,128 - +1.1 2,256,128 - Dsui_2127 0.62 -2.1 2,256,172 + Dsui_2127 0.67 +0.1 2,256,440 + -0.5 2,256,440 + +1.1 2,256,448 - -0.9 2,256,448 - -0.5 2,256,491 + -0.9 2,256,499 - +0.1 2,256,571 + +2.1
Or see this region's nucleotide sequence