Experiment: ALP with Fusidic 0.02 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Dsui_2116 and Dsui_2117 are separated by 160 nucleotides Dsui_2117 and Dsui_2118 are separated by 97 nucleotides
Dsui_2116: Dsui_2116 - Fe-dependent oxidoreductase, alcohol dehydrogenase, at 2,241,610 to 2,242,758
_2116
Dsui_2117: Dsui_2117 - acyl CoA:acetate/3-ketoacid CoA transferase, beta subunit, at 2,242,919 to 2,243,785
_2117
Dsui_2118: Dsui_2118 - acyl CoA:acetate/3-ketoacid CoA transferase, alpha subunit, at 2,243,883 to 2,244,890
_2118
Position (kb)
2242
2243
2244 Strain fitness (log2 ratio)
-2
-1
0
1 at 2242.053 kb on + strand at 2242.133 kb on - strand, within Dsui_2116 at 2242.330 kb on + strand, within Dsui_2116 at 2242.377 kb on + strand, within Dsui_2116 at 2242.406 kb on + strand at 2242.532 kb on - strand at 2242.580 kb on - strand at 2242.656 kb on + strand at 2242.668 kb on + strand at 2242.705 kb on + strand at 2242.705 kb on + strand at 2242.758 kb on + strand at 2242.761 kb on - strand at 2242.771 kb on + strand at 2242.781 kb on + strand at 2242.813 kb on - strand at 2242.895 kb on + strand at 2242.973 kb on - strand at 2243.024 kb on - strand at 2243.024 kb on - strand, within Dsui_2117 at 2243.076 kb on - strand at 2243.076 kb on - strand, within Dsui_2117 at 2243.270 kb on - strand, within Dsui_2117 at 2243.331 kb on + strand at 2243.337 kb on - strand at 2243.396 kb on - strand at 2243.396 kb on - strand, within Dsui_2117 at 2243.537 kb on - strand at 2243.712 kb on + strand at 2243.875 kb on - strand at 2243.929 kb on + strand at 2243.937 kb on - strand at 2244.115 kb on + strand at 2244.278 kb on - strand at 2244.310 kb on - strand at 2244.385 kb on + strand at 2244.583 kb on - strand, within Dsui_2118 at 2244.616 kb on + strand at 2244.673 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction ALP with Fusidic 0.02 mg/ml remove 2,242,053 + +1.1 2,242,133 - Dsui_2116 0.46 -1.9 2,242,330 + Dsui_2116 0.63 -1.2 2,242,377 + Dsui_2116 0.67 +1.1 2,242,406 + +1.1 2,242,532 - -0.5 2,242,580 - +1.1 2,242,656 + -0.5 2,242,668 + +1.1 2,242,705 + +1.1 2,242,705 + +1.1 2,242,758 + +0.5 2,242,761 - +0.1 2,242,771 + +0.1 2,242,781 + +1.1 2,242,813 - +1.1 2,242,895 + -0.9 2,242,973 - +0.1 2,243,024 - +1.1 2,243,024 - Dsui_2117 0.12 -0.5 2,243,076 - +1.1 2,243,076 - Dsui_2117 0.18 -0.9 2,243,270 - Dsui_2117 0.40 -0.5 2,243,331 + +1.1 2,243,337 - +1.1 2,243,396 - +1.1 2,243,396 - Dsui_2117 0.55 +1.1 2,243,537 - +1.1 2,243,712 + +1.1 2,243,875 - -1.2 2,243,929 + +1.1 2,243,937 - +1.1 2,244,115 + -0.9 2,244,278 - -0.5 2,244,310 - +0.1 2,244,385 + +1.1 2,244,583 - Dsui_2118 0.69 +1.1 2,244,616 + +0.1 2,244,673 + +1.1
Or see this region's nucleotide sequence