Experiment: ALP with Fusidic 0.02 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Dsui_2066 and Dsui_2067 are separated by 250 nucleotides Dsui_2067 and Dsui_2068 overlap by 8 nucleotides Dsui_2068 and Dsui_2069 are separated by 294 nucleotides
Dsui_2066: Dsui_2066 - ABC-type metal ion transport system, periplasmic component/surface antigen, at 2,184,345 to 2,185,148
_2066
Dsui_2067: Dsui_2067 - ABC-type metal ion transport system, permease component, at 2,185,399 to 2,186,097
_2067
Dsui_2068: Dsui_2068 - ABC-type metal ion transport system, ATPase component, at 2,186,090 to 2,186,926
_2068
Dsui_2069: Dsui_2069 - acyl-CoA synthetase (AMP-forming)/AMP-acid ligase II, at 2,187,221 to 2,188,810
_2069
Position (kb)
2186
2187 Strain fitness (log2 ratio)
-1
0
1 at 2185.135 kb on - strand at 2185.168 kb on - strand at 2185.168 kb on - strand at 2185.168 kb on - strand at 2185.223 kb on - strand at 2185.345 kb on + strand at 2185.389 kb on + strand at 2185.397 kb on - strand at 2185.419 kb on + strand at 2185.574 kb on - strand, within Dsui_2067 at 2185.579 kb on - strand, within Dsui_2067 at 2185.657 kb on + strand, within Dsui_2067 at 2185.673 kb on + strand, within Dsui_2067 at 2185.725 kb on + strand, within Dsui_2067 at 2185.920 kb on - strand, within Dsui_2067 at 2185.925 kb on - strand, within Dsui_2067 at 2185.931 kb on - strand, within Dsui_2067 at 2185.941 kb on - strand, within Dsui_2067 at 2186.092 kb on + strand at 2186.275 kb on + strand, within Dsui_2068 at 2186.433 kb on - strand, within Dsui_2068 at 2186.532 kb on + strand, within Dsui_2068 at 2186.640 kb on - strand, within Dsui_2068 at 2186.703 kb on - strand, within Dsui_2068 at 2186.733 kb on + strand, within Dsui_2068 at 2186.838 kb on + strand at 2186.853 kb on - strand at 2186.853 kb on - strand at 2186.949 kb on - strand at 2186.949 kb on - strand at 2186.949 kb on - strand at 2186.951 kb on - strand at 2186.959 kb on - strand at 2187.041 kb on - strand at 2187.065 kb on - strand at 2187.226 kb on - strand at 2187.355 kb on - strand at 2187.375 kb on - strand at 2187.375 kb on - strand at 2187.417 kb on - strand, within Dsui_2069 at 2187.484 kb on - strand, within Dsui_2069 at 2187.610 kb on - strand, within Dsui_2069 at 2187.710 kb on + strand at 2187.729 kb on + strand at 2187.755 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction ALP with Fusidic 0.02 mg/ml remove 2,185,135 - +0.5 2,185,168 - +0.4 2,185,168 - +1.2 2,185,168 - +0.2 2,185,223 - +0.1 2,185,345 + +1.1 2,185,389 + +1.1 2,185,397 - +0.2 2,185,419 + -0.5 2,185,574 - Dsui_2067 0.25 +0.6 2,185,579 - Dsui_2067 0.26 +0.6 2,185,657 + Dsui_2067 0.37 +0.1 2,185,673 + Dsui_2067 0.39 -1.5 2,185,725 + Dsui_2067 0.47 +0.1 2,185,920 - Dsui_2067 0.75 +0.4 2,185,925 - Dsui_2067 0.75 +0.8 2,185,931 - Dsui_2067 0.76 -0.1 2,185,941 - Dsui_2067 0.78 +1.0 2,186,092 + +1.1 2,186,275 + Dsui_2068 0.22 +1.1 2,186,433 - Dsui_2068 0.41 +0.5 2,186,532 + Dsui_2068 0.53 -0.5 2,186,640 - Dsui_2068 0.66 +0.6 2,186,703 - Dsui_2068 0.73 +0.4 2,186,733 + Dsui_2068 0.77 +0.1 2,186,838 + -0.9 2,186,853 - +0.7 2,186,853 - +0.1 2,186,949 - +0.9 2,186,949 - +0.3 2,186,949 - +1.0 2,186,951 - +1.1 2,186,959 - +0.9 2,187,041 - +0.6 2,187,065 - +0.1 2,187,226 - -1.2 2,187,355 - +1.5 2,187,375 - +0.1 2,187,375 - +0.7 2,187,417 - Dsui_2069 0.12 +1.1 2,187,484 - Dsui_2069 0.17 +1.7 2,187,610 - Dsui_2069 0.24 +0.2 2,187,710 + -0.5 2,187,729 + +0.1 2,187,755 + +0.1
Or see this region's nucleotide sequence