Experiment: ALP with Fusidic 0.02 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Dsui_1877 and Dsui_1878 are separated by 69 nucleotides Dsui_1878 and Dsui_1879 overlap by 4 nucleotides
Dsui_1877: Dsui_1877 - NADH:flavin oxidoreductase, at 1,974,447 to 1,975,529
_1877
Dsui_1878: Dsui_1878 - DMT(drug/metabolite transporter) superfamily permease, at 1,975,599 to 1,976,528
_1878
Dsui_1879: Dsui_1879 - luciferase family oxidoreductase, group 1, at 1,976,525 to 1,977,526
_1879
Position (kb)
1975
1976
1977 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 1974.606 kb on + strand, within Dsui_1877 at 1974.611 kb on + strand, within Dsui_1877 at 1974.633 kb on + strand at 1974.660 kb on - strand at 1974.792 kb on + strand at 1974.800 kb on + strand, within Dsui_1877 at 1974.929 kb on - strand, within Dsui_1877 at 1975.152 kb on + strand, within Dsui_1877 at 1975.262 kb on + strand, within Dsui_1877 at 1975.425 kb on - strand at 1975.499 kb on + strand at 1975.518 kb on + strand at 1975.521 kb on - strand at 1975.523 kb on + strand at 1975.523 kb on + strand at 1975.531 kb on - strand at 1975.536 kb on + strand at 1975.536 kb on + strand at 1975.536 kb on + strand at 1975.536 kb on + strand at 1975.654 kb on + strand at 1975.666 kb on + strand at 1975.666 kb on + strand at 1975.674 kb on - strand at 1975.808 kb on - strand, within Dsui_1878 at 1975.818 kb on + strand, within Dsui_1878 at 1975.855 kb on + strand, within Dsui_1878 at 1975.858 kb on + strand, within Dsui_1878 at 1975.865 kb on + strand, within Dsui_1878 at 1975.865 kb on + strand at 1975.865 kb on + strand, within Dsui_1878 at 1975.880 kb on + strand, within Dsui_1878 at 1975.929 kb on - strand, within Dsui_1878 at 1975.932 kb on + strand, within Dsui_1878 at 1975.944 kb on - strand at 1976.083 kb on - strand, within Dsui_1878 at 1976.099 kb on + strand, within Dsui_1878 at 1976.175 kb on + strand, within Dsui_1878 at 1976.185 kb on + strand at 1976.278 kb on + strand at 1976.310 kb on + strand, within Dsui_1878 at 1976.372 kb on - strand, within Dsui_1878 at 1976.426 kb on + strand at 1976.437 kb on + strand at 1976.450 kb on - strand at 1976.547 kb on + strand at 1976.550 kb on + strand at 1976.550 kb on - strand at 1976.600 kb on + strand at 1976.607 kb on - strand at 1976.607 kb on - strand at 1976.682 kb on - strand, within Dsui_1879 at 1976.790 kb on + strand, within Dsui_1879 at 1976.852 kb on + strand at 1976.931 kb on - strand, within Dsui_1879 at 1977.039 kb on + strand, within Dsui_1879 at 1977.142 kb on - strand at 1977.162 kb on + strand, within Dsui_1879 at 1977.200 kb on + strand, within Dsui_1879 at 1977.262 kb on + strand, within Dsui_1879 at 1977.328 kb on + strand at 1977.329 kb on + strand, within Dsui_1879 at 1977.492 kb on + strand at 1977.519 kb on - strand at 1977.522 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction ALP with Fusidic 0.02 mg/ml remove 1,974,606 + Dsui_1877 0.15 +0.9 1,974,611 + Dsui_1877 0.15 -1.1 1,974,633 + -0.6 1,974,660 - -0.6 1,974,792 + +0.9 1,974,800 + Dsui_1877 0.33 +0.9 1,974,929 - Dsui_1877 0.45 -1.1 1,975,152 + Dsui_1877 0.65 +0.9 1,975,262 + Dsui_1877 0.75 -2.8 1,975,425 - -1.4 1,975,499 + +0.9 1,975,518 + +0.9 1,975,521 - -0.1 1,975,523 + -0.6 1,975,523 + +0.9 1,975,531 - +0.9 1,975,536 + -0.6 1,975,536 + -0.1 1,975,536 + -0.1 1,975,536 + -0.6 1,975,654 + +0.9 1,975,666 + +0.9 1,975,666 + -1.4 1,975,674 - -2.1 1,975,808 - Dsui_1878 0.22 +0.9 1,975,818 + Dsui_1878 0.24 -0.6 1,975,855 + Dsui_1878 0.28 -0.6 1,975,858 + Dsui_1878 0.28 -0.1 1,975,865 + Dsui_1878 0.29 -0.1 1,975,865 + +0.9 1,975,865 + Dsui_1878 0.29 +0.9 1,975,880 + Dsui_1878 0.30 -0.6 1,975,929 - Dsui_1878 0.35 -0.1 1,975,932 + Dsui_1878 0.36 -1.4 1,975,944 - -0.1 1,976,083 - Dsui_1878 0.52 -0.6 1,976,099 + Dsui_1878 0.54 +0.9 1,976,175 + Dsui_1878 0.62 -1.1 1,976,185 + +0.9 1,976,278 + +0.9 1,976,310 + Dsui_1878 0.76 -0.6 1,976,372 - Dsui_1878 0.83 -1.4 1,976,426 + +0.9 1,976,437 + -1.6 1,976,450 - -0.6 1,976,547 + -1.4 1,976,550 + -1.4 1,976,550 - +0.9 1,976,600 + -0.1 1,976,607 - -0.1 1,976,607 - -0.6 1,976,682 - Dsui_1879 0.16 -1.4 1,976,790 + Dsui_1879 0.26 -0.1 1,976,852 + +0.9 1,976,931 - Dsui_1879 0.41 -0.1 1,977,039 + Dsui_1879 0.51 -0.1 1,977,142 - -0.1 1,977,162 + Dsui_1879 0.64 -1.4 1,977,200 + Dsui_1879 0.67 -0.6 1,977,262 + Dsui_1879 0.74 -0.6 1,977,328 + -1.1 1,977,329 + Dsui_1879 0.80 -1.4 1,977,492 + -0.1 1,977,519 - +0.9 1,977,522 + +0.9
Or see this region's nucleotide sequence