Experiment: ALP with Fusidic 0.02 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Dsui_1872 and Dsui_1873 are separated by 170 nucleotides Dsui_1873 and Dsui_1874 overlap by 1 nucleotides Dsui_1874 and Dsui_1875 are separated by 66 nucleotides Dsui_1875 and Dsui_1876 are separated by 146 nucleotides
Dsui_1872: Dsui_1872 - RND family efflux transporter, MFP subunit, at 1,971,002 to 1,972,261
_1872
Dsui_1873: Dsui_1873 - transcriptional regulator, at 1,972,432 to 1,973,046
_1873
Dsui_1874: Dsui_1874 - hypothetical protein, at 1,973,046 to 1,973,378
_1874
Dsui_1875: Dsui_1875 - hypothetical protein, at 1,973,445 to 1,973,618
_1875
Dsui_1876: Dsui_1876 - transcriptional regulator, at 1,973,765 to 1,974,349
_1876
Position (kb)
1972
1973
1974 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3
4
5
6
7
8
9
10 at 1971.537 kb on - strand, within Dsui_1872 at 1971.585 kb on + strand, within Dsui_1872 at 1971.593 kb on - strand at 1971.593 kb on - strand, within Dsui_1872 at 1971.615 kb on - strand, within Dsui_1872 at 1971.665 kb on - strand, within Dsui_1872 at 1971.669 kb on - strand, within Dsui_1872 at 1971.677 kb on - strand, within Dsui_1872 at 1971.679 kb on - strand, within Dsui_1872 at 1971.759 kb on - strand, within Dsui_1872 at 1971.765 kb on - strand, within Dsui_1872 at 1971.783 kb on + strand, within Dsui_1872 at 1971.797 kb on + strand, within Dsui_1872 at 1971.914 kb on - strand, within Dsui_1872 at 1971.914 kb on - strand, within Dsui_1872 at 1971.928 kb on + strand at 1971.983 kb on - strand, within Dsui_1872 at 1972.064 kb on - strand, within Dsui_1872 at 1972.075 kb on + strand, within Dsui_1872 at 1972.075 kb on + strand at 1972.089 kb on + strand at 1972.127 kb on - strand, within Dsui_1872 at 1972.130 kb on + strand, within Dsui_1872 at 1972.130 kb on + strand at 1972.153 kb on - strand at 1972.234 kb on - strand at 1972.239 kb on + strand at 1972.390 kb on - strand at 1972.548 kb on + strand, within Dsui_1873 at 1972.556 kb on + strand, within Dsui_1873 at 1972.595 kb on + strand, within Dsui_1873 at 1972.597 kb on + strand, within Dsui_1873 at 1972.739 kb on + strand, within Dsui_1873 at 1972.859 kb on + strand, within Dsui_1873 at 1972.925 kb on + strand, within Dsui_1873 at 1973.055 kb on + strand at 1973.167 kb on + strand, within Dsui_1874 at 1973.167 kb on + strand, within Dsui_1874 at 1973.173 kb on + strand, within Dsui_1874 at 1973.181 kb on - strand, within Dsui_1874 at 1973.233 kb on - strand, within Dsui_1874 at 1973.270 kb on - strand, within Dsui_1874 at 1973.334 kb on - strand, within Dsui_1874 at 1973.356 kb on + strand at 1973.438 kb on + strand at 1973.526 kb on - strand, within Dsui_1875 at 1973.545 kb on - strand, within Dsui_1875 at 1973.581 kb on - strand, within Dsui_1875 at 1973.601 kb on + strand at 1973.601 kb on + strand at 1973.645 kb on - strand at 1973.828 kb on + strand, within Dsui_1876 at 1973.892 kb on + strand, within Dsui_1876
Per-strain Table
Position Strand Gene LocusTag Fraction ALP with Fusidic 0.02 mg/ml remove 1,971,537 - Dsui_1872 0.42 -2.2 1,971,585 + Dsui_1872 0.46 -0.6 1,971,593 - +0.9 1,971,593 - Dsui_1872 0.47 -2.2 1,971,615 - Dsui_1872 0.49 -0.9 1,971,665 - Dsui_1872 0.53 -1.6 1,971,669 - Dsui_1872 0.53 -1.1 1,971,677 - Dsui_1872 0.54 -1.6 1,971,679 - Dsui_1872 0.54 -1.4 1,971,759 - Dsui_1872 0.60 -4.2 1,971,765 - Dsui_1872 0.61 -2.5 1,971,783 + Dsui_1872 0.62 -1.6 1,971,797 + Dsui_1872 0.63 -0.1 1,971,914 - Dsui_1872 0.72 -1.6 1,971,914 - Dsui_1872 0.72 -0.9 1,971,928 + +0.9 1,971,983 - Dsui_1872 0.78 -0.3 1,972,064 - Dsui_1872 0.84 -1.1 1,972,075 + Dsui_1872 0.85 -1.4 1,972,075 + -0.6 1,972,089 + +0.9 1,972,127 - Dsui_1872 0.89 -3.1 1,972,130 + Dsui_1872 0.90 -0.6 1,972,130 + -0.1 1,972,153 - -0.6 1,972,234 - -3.8 1,972,239 + -1.4 1,972,390 - -0.1 1,972,548 + Dsui_1873 0.19 +10.0 1,972,556 + Dsui_1873 0.20 +9.9 1,972,595 + Dsui_1873 0.27 +10.1 1,972,597 + Dsui_1873 0.27 +10.0 1,972,739 + Dsui_1873 0.50 +10.0 1,972,859 + Dsui_1873 0.69 +9.8 1,972,925 + Dsui_1873 0.80 +9.5 1,973,055 + +1.5 1,973,167 + Dsui_1874 0.36 +1.5 1,973,167 + Dsui_1874 0.36 -2.3 1,973,173 + Dsui_1874 0.38 +0.4 1,973,181 - Dsui_1874 0.41 +0.9 1,973,233 - Dsui_1874 0.56 +0.9 1,973,270 - Dsui_1874 0.67 +0.9 1,973,334 - Dsui_1874 0.86 -1.6 1,973,356 + +0.6 1,973,438 + -1.1 1,973,526 - Dsui_1875 0.47 +0.0 1,973,545 - Dsui_1875 0.57 -0.1 1,973,581 - Dsui_1875 0.78 -0.2 1,973,601 + -1.1 1,973,601 + -1.4 1,973,645 - +0.9 1,973,828 + Dsui_1876 0.11 +0.6 1,973,892 + Dsui_1876 0.22 +0.3
Or see this region's nucleotide sequence