Experiment: ALP with Fusidic 0.02 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Dsui_1845 and Dsui_1846 are separated by 101 nucleotides Dsui_1846 and Dsui_1847 are separated by 61 nucleotides Dsui_1847 and Dsui_1848 overlap by 17 nucleotides
Dsui_1845: Dsui_1845 - phosphate/sulfate permease, at 1,940,317 to 1,941,360
_1845
Dsui_1846: Dsui_1846 - outer membrane phospholipase A, at 1,941,462 to 1,942,622
_1846
Dsui_1847: Dsui_1847 - acetyltransferase, N-acetylglutamate synthase, at 1,942,684 to 1,943,136
_1847
Dsui_1848: Dsui_1848 - arsenical-resistance protein, at 1,943,120 to 1,944,175
_1848
Position (kb)
1941
1942
1943 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1 at 1940.485 kb on - strand, within Dsui_1845 at 1940.664 kb on - strand at 1940.718 kb on + strand at 1940.718 kb on + strand at 1940.897 kb on - strand, within Dsui_1845 at 1940.962 kb on + strand, within Dsui_1845 at 1941.061 kb on + strand at 1941.069 kb on - strand, within Dsui_1845 at 1941.096 kb on - strand, within Dsui_1845 at 1941.189 kb on - strand, within Dsui_1845 at 1941.573 kb on - strand at 1941.606 kb on - strand, within Dsui_1846 at 1941.717 kb on - strand, within Dsui_1846 at 1941.735 kb on - strand, within Dsui_1846 at 1941.769 kb on - strand, within Dsui_1846 at 1941.781 kb on - strand, within Dsui_1846 at 1941.886 kb on + strand at 1941.955 kb on - strand, within Dsui_1846 at 1942.021 kb on - strand, within Dsui_1846 at 1942.044 kb on + strand, within Dsui_1846 at 1942.052 kb on - strand, within Dsui_1846 at 1942.053 kb on - strand, within Dsui_1846 at 1942.131 kb on + strand, within Dsui_1846 at 1942.162 kb on - strand, within Dsui_1846 at 1942.309 kb on - strand, within Dsui_1846 at 1942.336 kb on + strand, within Dsui_1846 at 1942.391 kb on + strand at 1942.816 kb on + strand at 1942.897 kb on - strand, within Dsui_1847 at 1942.903 kb on - strand at 1942.942 kb on + strand, within Dsui_1847 at 1943.031 kb on + strand at 1943.058 kb on + strand at 1943.058 kb on + strand at 1943.066 kb on - strand, within Dsui_1847 at 1943.097 kb on - strand at 1943.136 kb on + strand at 1943.213 kb on + strand at 1943.299 kb on - strand, within Dsui_1848 at 1943.463 kb on - strand at 1943.608 kb on + strand at 1943.621 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction ALP with Fusidic 0.02 mg/ml remove 1,940,485 - Dsui_1845 0.16 -0.2 1,940,664 - +0.8 1,940,718 + +0.8 1,940,718 + +0.8 1,940,897 - Dsui_1845 0.56 -1.5 1,940,962 + Dsui_1845 0.62 -0.2 1,941,061 + +0.8 1,941,069 - Dsui_1845 0.72 +0.8 1,941,096 - Dsui_1845 0.75 +0.8 1,941,189 - Dsui_1845 0.84 -1.8 1,941,573 - +0.1 1,941,606 - Dsui_1846 0.12 -2.1 1,941,717 - Dsui_1846 0.22 -4.0 1,941,735 - Dsui_1846 0.24 -1.0 1,941,769 - Dsui_1846 0.26 -2.9 1,941,781 - Dsui_1846 0.27 -1.5 1,941,886 + +0.8 1,941,955 - Dsui_1846 0.42 -1.5 1,942,021 - Dsui_1846 0.48 +1.3 1,942,044 + Dsui_1846 0.50 -1.5 1,942,052 - Dsui_1846 0.51 -2.2 1,942,053 - Dsui_1846 0.51 +0.8 1,942,131 + Dsui_1846 0.58 -1.2 1,942,162 - Dsui_1846 0.60 -1.8 1,942,309 - Dsui_1846 0.73 +0.8 1,942,336 + Dsui_1846 0.75 -0.2 1,942,391 + +0.8 1,942,816 + -0.8 1,942,897 - Dsui_1847 0.47 -1.2 1,942,903 - -0.2 1,942,942 + Dsui_1847 0.57 -0.2 1,943,031 + +0.8 1,943,058 + +0.8 1,943,058 + -0.2 1,943,066 - Dsui_1847 0.84 -1.8 1,943,097 - -0.2 1,943,136 + -1.5 1,943,213 + -1.2 1,943,299 - Dsui_1848 0.17 -0.8 1,943,463 - -0.8 1,943,608 + +0.8 1,943,621 - +0.8
Or see this region's nucleotide sequence