Experiment: ALP with Fusidic 0.02 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Dsui_1775 and Dsui_1776 are separated by 59 nucleotides Dsui_1776 and Dsui_1777 are separated by 66 nucleotides Dsui_1777 and Dsui_1778 overlap by 23 nucleotides
Dsui_1775: Dsui_1775 - type I secretion membrane fusion protein, HlyD family, at 1,866,496 to 1,867,905
_1775
Dsui_1776: Dsui_1776 - putative glycosyltransferase, at 1,867,965 to 1,868,777
_1776
Dsui_1777: Dsui_1777 - uncharacterized protein, cytoplasmic domain of flagellar protein FhlB like protein, at 1,868,844 to 1,869,164
_1777
Dsui_1778: Dsui_1778 - Flagellar hook-length control protein FliK, at 1,869,142 to 1,870,320
_1778
Position (kb)
1868
1869
1870 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3 at 1867.864 kb on + strand at 1867.866 kb on - strand at 1867.875 kb on + strand at 1867.901 kb on + strand at 1867.914 kb on - strand at 1867.971 kb on - strand at 1867.971 kb on - strand at 1867.980 kb on + strand at 1867.986 kb on + strand at 1868.007 kb on - strand at 1868.017 kb on - strand at 1868.101 kb on - strand, within Dsui_1776 at 1868.116 kb on - strand, within Dsui_1776 at 1868.139 kb on - strand, within Dsui_1776 at 1868.167 kb on - strand, within Dsui_1776 at 1868.199 kb on + strand, within Dsui_1776 at 1868.207 kb on - strand, within Dsui_1776 at 1868.207 kb on - strand, within Dsui_1776 at 1868.207 kb on - strand, within Dsui_1776 at 1868.207 kb on - strand, within Dsui_1776 at 1868.220 kb on - strand, within Dsui_1776 at 1868.326 kb on + strand, within Dsui_1776 at 1868.377 kb on + strand, within Dsui_1776 at 1868.377 kb on + strand, within Dsui_1776 at 1868.385 kb on - strand, within Dsui_1776 at 1868.434 kb on - strand, within Dsui_1776 at 1868.445 kb on + strand, within Dsui_1776 at 1868.453 kb on - strand, within Dsui_1776 at 1868.463 kb on + strand, within Dsui_1776 at 1868.488 kb on - strand, within Dsui_1776 at 1868.579 kb on + strand, within Dsui_1776 at 1868.581 kb on - strand, within Dsui_1776 at 1868.585 kb on + strand, within Dsui_1776 at 1868.588 kb on + strand, within Dsui_1776 at 1868.592 kb on + strand, within Dsui_1776 at 1868.592 kb on + strand, within Dsui_1776 at 1868.593 kb on - strand, within Dsui_1776 at 1868.600 kb on - strand, within Dsui_1776 at 1868.617 kb on + strand, within Dsui_1776 at 1868.670 kb on - strand, within Dsui_1776 at 1868.674 kb on + strand, within Dsui_1776 at 1868.674 kb on + strand, within Dsui_1776 at 1868.674 kb on + strand, within Dsui_1776 at 1868.682 kb on - strand, within Dsui_1776 at 1868.684 kb on + strand, within Dsui_1776 at 1868.692 kb on - strand, within Dsui_1776 at 1868.708 kb on - strand at 1868.734 kb on + strand at 1868.739 kb on + strand at 1868.740 kb on + strand at 1868.754 kb on + strand at 1868.755 kb on - strand at 1868.767 kb on - strand at 1868.769 kb on - strand at 1868.773 kb on + strand at 1868.790 kb on + strand at 1868.800 kb on + strand at 1868.801 kb on + strand at 1868.807 kb on + strand at 1868.808 kb on + strand at 1868.808 kb on - strand at 1868.810 kb on - strand at 1868.810 kb on - strand at 1868.810 kb on - strand at 1868.815 kb on - strand at 1868.828 kb on + strand at 1868.836 kb on - strand at 1868.851 kb on - strand at 1868.858 kb on + strand at 1868.858 kb on + strand at 1868.858 kb on + strand at 1868.866 kb on - strand at 1868.866 kb on - strand at 1868.866 kb on - strand at 1868.866 kb on - strand at 1868.879 kb on - strand, within Dsui_1777 at 1868.919 kb on + strand, within Dsui_1777 at 1868.951 kb on + strand, within Dsui_1777 at 1868.951 kb on - strand, within Dsui_1777 at 1868.956 kb on - strand, within Dsui_1777 at 1868.956 kb on - strand, within Dsui_1777 at 1868.970 kb on - strand, within Dsui_1777 at 1869.060 kb on - strand, within Dsui_1777 at 1869.105 kb on - strand, within Dsui_1777 at 1869.114 kb on + strand, within Dsui_1777 at 1869.182 kb on - strand at 1869.182 kb on - strand at 1869.183 kb on + strand at 1869.191 kb on - strand at 1869.222 kb on + strand at 1869.230 kb on - strand at 1869.265 kb on + strand, within Dsui_1778 at 1869.280 kb on - strand, within Dsui_1778 at 1869.346 kb on - strand, within Dsui_1778 at 1869.348 kb on + strand, within Dsui_1778 at 1869.359 kb on - strand, within Dsui_1778 at 1869.365 kb on + strand, within Dsui_1778 at 1869.439 kb on - strand, within Dsui_1778 at 1869.446 kb on + strand, within Dsui_1778 at 1869.446 kb on + strand, within Dsui_1778 at 1869.454 kb on - strand, within Dsui_1778 at 1869.454 kb on - strand, within Dsui_1778 at 1869.454 kb on - strand, within Dsui_1778 at 1869.459 kb on - strand, within Dsui_1778 at 1869.466 kb on + strand, within Dsui_1778 at 1869.473 kb on - strand, within Dsui_1778 at 1869.473 kb on - strand, within Dsui_1778 at 1869.475 kb on - strand, within Dsui_1778 at 1869.481 kb on - strand, within Dsui_1778 at 1869.490 kb on + strand, within Dsui_1778 at 1869.512 kb on - strand, within Dsui_1778 at 1869.538 kb on - strand, within Dsui_1778 at 1869.595 kb on - strand, within Dsui_1778 at 1869.605 kb on + strand, within Dsui_1778 at 1869.648 kb on + strand, within Dsui_1778 at 1869.656 kb on - strand, within Dsui_1778 at 1869.667 kb on + strand, within Dsui_1778 at 1869.678 kb on + strand, within Dsui_1778 at 1869.700 kb on + strand, within Dsui_1778 at 1869.701 kb on - strand, within Dsui_1778 at 1869.718 kb on - strand, within Dsui_1778 at 1869.743 kb on + strand, within Dsui_1778 at 1869.748 kb on + strand, within Dsui_1778 at 1869.751 kb on - strand, within Dsui_1778 at 1869.764 kb on + strand, within Dsui_1778 at 1869.764 kb on + strand, within Dsui_1778 at 1869.783 kb on + strand, within Dsui_1778 at 1869.808 kb on + strand, within Dsui_1778 at 1869.816 kb on - strand, within Dsui_1778 at 1869.816 kb on - strand, within Dsui_1778 at 1869.816 kb on - strand, within Dsui_1778 at 1869.893 kb on - strand, within Dsui_1778 at 1869.914 kb on + strand, within Dsui_1778 at 1869.922 kb on - strand, within Dsui_1778 at 1869.931 kb on - strand, within Dsui_1778 at 1869.934 kb on + strand, within Dsui_1778 at 1869.942 kb on - strand, within Dsui_1778 at 1869.942 kb on - strand, within Dsui_1778 at 1869.942 kb on - strand, within Dsui_1778 at 1869.944 kb on - strand, within Dsui_1778 at 1869.948 kb on - strand, within Dsui_1778 at 1869.956 kb on + strand, within Dsui_1778 at 1869.964 kb on - strand, within Dsui_1778 at 1869.966 kb on - strand, within Dsui_1778 at 1869.979 kb on - strand, within Dsui_1778 at 1870.045 kb on - strand, within Dsui_1778 at 1870.125 kb on - strand, within Dsui_1778 at 1870.130 kb on + strand, within Dsui_1778 at 1870.138 kb on - strand, within Dsui_1778 at 1870.151 kb on + strand, within Dsui_1778
Per-strain Table
Position Strand Gene LocusTag Fraction ALP with Fusidic 0.02 mg/ml remove 1,867,864 + +0.6 1,867,866 - +0.7 1,867,875 + +0.2 1,867,901 + -0.7 1,867,914 - -0.3 1,867,971 - -0.8 1,867,971 - +0.5 1,867,980 + +0.7 1,867,986 + -0.9 1,868,007 - +0.3 1,868,017 - -0.2 1,868,101 - Dsui_1776 0.17 -0.1 1,868,116 - Dsui_1776 0.19 +0.2 1,868,139 - Dsui_1776 0.21 -0.4 1,868,167 - Dsui_1776 0.25 -0.2 1,868,199 + Dsui_1776 0.29 -0.9 1,868,207 - Dsui_1776 0.30 -0.4 1,868,207 - Dsui_1776 0.30 -0.2 1,868,207 - Dsui_1776 0.30 +0.1 1,868,207 - Dsui_1776 0.30 -0.3 1,868,220 - Dsui_1776 0.31 -0.3 1,868,326 + Dsui_1776 0.44 -0.9 1,868,377 + Dsui_1776 0.51 -0.9 1,868,377 + Dsui_1776 0.51 -0.3 1,868,385 - Dsui_1776 0.52 -0.3 1,868,434 - Dsui_1776 0.58 -0.4 1,868,445 + Dsui_1776 0.59 -0.2 1,868,453 - Dsui_1776 0.60 +0.3 1,868,463 + Dsui_1776 0.61 -0.9 1,868,488 - Dsui_1776 0.64 -1.8 1,868,579 + Dsui_1776 0.76 -1.7 1,868,581 - Dsui_1776 0.76 -0.3 1,868,585 + Dsui_1776 0.76 +0.7 1,868,588 + Dsui_1776 0.77 -1.3 1,868,592 + Dsui_1776 0.77 +0.7 1,868,592 + Dsui_1776 0.77 -0.3 1,868,593 - Dsui_1776 0.77 +0.0 1,868,600 - Dsui_1776 0.78 -0.3 1,868,617 + Dsui_1776 0.80 +0.7 1,868,670 - Dsui_1776 0.87 -0.6 1,868,674 + Dsui_1776 0.87 -0.3 1,868,674 + Dsui_1776 0.87 -2.3 1,868,674 + Dsui_1776 0.87 -2.3 1,868,682 - Dsui_1776 0.88 -0.3 1,868,684 + Dsui_1776 0.88 -1.7 1,868,692 - Dsui_1776 0.89 +1.0 1,868,708 - -0.2 1,868,734 + -0.3 1,868,739 + -0.3 1,868,740 + +0.7 1,868,754 + -1.9 1,868,755 - -0.3 1,868,767 - -0.1 1,868,769 - -0.2 1,868,773 + +0.7 1,868,790 + -0.9 1,868,800 + -0.3 1,868,801 + -0.9 1,868,807 + +0.7 1,868,808 + +0.7 1,868,808 - +0.4 1,868,810 - +0.3 1,868,810 - -0.1 1,868,810 - +0.7 1,868,815 - +0.5 1,868,828 + +0.7 1,868,836 - -3.9 1,868,851 - -0.3 1,868,858 + -0.3 1,868,858 + -0.9 1,868,858 + -0.9 1,868,866 - -0.9 1,868,866 - -1.3 1,868,866 - -0.9 1,868,866 - +0.7 1,868,879 - Dsui_1777 0.11 -0.9 1,868,919 + Dsui_1777 0.23 +0.7 1,868,951 + Dsui_1777 0.33 -1.7 1,868,951 - Dsui_1777 0.33 -0.9 1,868,956 - Dsui_1777 0.35 -0.3 1,868,956 - Dsui_1777 0.35 +0.7 1,868,970 - Dsui_1777 0.39 +0.7 1,869,060 - Dsui_1777 0.67 -2.7 1,869,105 - Dsui_1777 0.81 +0.7 1,869,114 + Dsui_1777 0.84 +0.7 1,869,182 - +2.0 1,869,182 - -1.7 1,869,183 + -1.3 1,869,191 - -1.3 1,869,222 + -2.8 1,869,230 - -0.9 1,869,265 + Dsui_1778 0.10 +0.7 1,869,280 - Dsui_1778 0.12 -2.8 1,869,346 - Dsui_1778 0.17 +0.7 1,869,348 + Dsui_1778 0.17 -1.3 1,869,359 - Dsui_1778 0.18 -0.3 1,869,365 + Dsui_1778 0.19 -1.7 1,869,439 - Dsui_1778 0.25 -0.3 1,869,446 + Dsui_1778 0.26 -1.3 1,869,446 + Dsui_1778 0.26 +0.7 1,869,454 - Dsui_1778 0.26 +0.7 1,869,454 - Dsui_1778 0.26 -0.3 1,869,454 - Dsui_1778 0.26 -0.3 1,869,459 - Dsui_1778 0.27 -0.3 1,869,466 + Dsui_1778 0.27 -0.9 1,869,473 - Dsui_1778 0.28 +0.7 1,869,473 - Dsui_1778 0.28 -0.3 1,869,475 - Dsui_1778 0.28 -0.3 1,869,481 - Dsui_1778 0.29 +1.7 1,869,490 + Dsui_1778 0.30 +0.7 1,869,512 - Dsui_1778 0.31 +0.7 1,869,538 - Dsui_1778 0.34 +3.3 1,869,595 - Dsui_1778 0.38 -2.5 1,869,605 + Dsui_1778 0.39 -0.3 1,869,648 + Dsui_1778 0.43 +0.7 1,869,656 - Dsui_1778 0.44 -1.6 1,869,667 + Dsui_1778 0.45 +0.7 1,869,678 + Dsui_1778 0.45 -1.6 1,869,700 + Dsui_1778 0.47 +0.7 1,869,701 - Dsui_1778 0.47 -1.9 1,869,718 - Dsui_1778 0.49 -0.9 1,869,743 + Dsui_1778 0.51 +0.7 1,869,748 + Dsui_1778 0.51 -0.3 1,869,751 - Dsui_1778 0.52 -1.6 1,869,764 + Dsui_1778 0.53 -0.3 1,869,764 + Dsui_1778 0.53 -2.1 1,869,783 + Dsui_1778 0.54 +0.7 1,869,808 + Dsui_1778 0.56 -0.3 1,869,816 - Dsui_1778 0.57 -1.3 1,869,816 - Dsui_1778 0.57 -0.3 1,869,816 - Dsui_1778 0.57 +0.7 1,869,893 - Dsui_1778 0.64 +0.7 1,869,914 + Dsui_1778 0.65 +0.7 1,869,922 - Dsui_1778 0.66 -1.9 1,869,931 - Dsui_1778 0.67 -2.1 1,869,934 + Dsui_1778 0.67 +0.7 1,869,942 - Dsui_1778 0.68 -2.5 1,869,942 - Dsui_1778 0.68 -2.6 1,869,942 - Dsui_1778 0.68 -2.3 1,869,944 - Dsui_1778 0.68 +0.7 1,869,948 - Dsui_1778 0.68 -2.6 1,869,956 + Dsui_1778 0.69 +0.7 1,869,964 - Dsui_1778 0.70 +0.7 1,869,966 - Dsui_1778 0.70 -2.3 1,869,979 - Dsui_1778 0.71 +0.7 1,870,045 - Dsui_1778 0.77 -1.6 1,870,125 - Dsui_1778 0.83 -0.9 1,870,130 + Dsui_1778 0.84 -0.3 1,870,138 - Dsui_1778 0.84 -1.1 1,870,151 + Dsui_1778 0.86 -1.3
Or see this region's nucleotide sequence