Experiment: ALP with Fusidic 0.02 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Dsui_1761 and Dsui_1762 are separated by 42 nucleotides Dsui_1762 and Dsui_1763 are separated by 73 nucleotides Dsui_1763 and Dsui_1764 are separated by 34 nucleotides Dsui_1764 and Dsui_1765 overlap by 11 nucleotides Dsui_1765 and Dsui_1766 overlap by 8 nucleotides
Dsui_1761: Dsui_1761 - methylase involved in ubiquinone/menaquinone biosynthesis, at 1,849,326 to 1,850,357
_1761
Dsui_1762: Dsui_1762 - methylase involved in ubiquinone/menaquinone biosynthesis, at 1,850,400 to 1,850,894
_1762
Dsui_1763: Dsui_1763 - subfamily IIIC HAD-superfamily phosphatase, at 1,850,968 to 1,852,668
_1763
Dsui_1764: Dsui_1764 - acyl carrier protein, at 1,852,703 to 1,852,915
_1764
Dsui_1765: Dsui_1765 - acetyltransferase, ribosomal protein N-acetylase, at 1,852,905 to 1,853,489
_1765
Dsui_1766: Dsui_1766 - hypothetical protein, at 1,853,482 to 1,853,910
_1766
Position (kb)
1850
1851
1852
1853 Strain fitness (log2 ratio)
-2
-1
0
1 at 1850.052 kb on - strand, within Dsui_1761 at 1850.052 kb on - strand, within Dsui_1761 at 1850.052 kb on - strand, within Dsui_1761 at 1850.082 kb on - strand, within Dsui_1761 at 1850.090 kb on + strand, within Dsui_1761 at 1850.094 kb on - strand, within Dsui_1761 at 1850.118 kb on - strand, within Dsui_1761 at 1850.216 kb on - strand, within Dsui_1761 at 1850.314 kb on - strand at 1850.319 kb on - strand at 1850.410 kb on + strand at 1850.415 kb on + strand at 1850.436 kb on + strand at 1850.465 kb on + strand, within Dsui_1762 at 1850.522 kb on - strand, within Dsui_1762 at 1850.530 kb on - strand, within Dsui_1762 at 1850.536 kb on - strand, within Dsui_1762 at 1850.592 kb on - strand, within Dsui_1762 at 1850.595 kb on + strand, within Dsui_1762 at 1850.618 kb on + strand, within Dsui_1762 at 1850.651 kb on - strand, within Dsui_1762 at 1850.701 kb on + strand, within Dsui_1762 at 1850.756 kb on + strand, within Dsui_1762 at 1850.757 kb on + strand, within Dsui_1762 at 1850.758 kb on + strand, within Dsui_1762 at 1850.758 kb on + strand, within Dsui_1762 at 1850.764 kb on - strand at 1850.766 kb on - strand, within Dsui_1762 at 1850.781 kb on + strand, within Dsui_1762 at 1850.815 kb on - strand, within Dsui_1762 at 1851.000 kb on + strand at 1851.151 kb on + strand, within Dsui_1763 at 1851.276 kb on + strand, within Dsui_1763 at 1851.285 kb on - strand, within Dsui_1763 at 1851.312 kb on - strand, within Dsui_1763 at 1851.326 kb on + strand, within Dsui_1763 at 1851.479 kb on - strand, within Dsui_1763 at 1851.530 kb on - strand, within Dsui_1763 at 1851.542 kb on + strand, within Dsui_1763 at 1851.568 kb on + strand, within Dsui_1763 at 1851.630 kb on - strand, within Dsui_1763 at 1851.687 kb on + strand, within Dsui_1763 at 1851.709 kb on + strand, within Dsui_1763 at 1851.811 kb on + strand, within Dsui_1763 at 1851.852 kb on + strand, within Dsui_1763 at 1851.922 kb on - strand, within Dsui_1763 at 1852.009 kb on + strand, within Dsui_1763 at 1852.009 kb on + strand, within Dsui_1763 at 1852.017 kb on - strand, within Dsui_1763 at 1852.035 kb on - strand, within Dsui_1763 at 1852.167 kb on + strand, within Dsui_1763 at 1852.175 kb on - strand, within Dsui_1763 at 1852.272 kb on + strand, within Dsui_1763 at 1852.327 kb on - strand at 1852.377 kb on + strand, within Dsui_1763 at 1852.551 kb on - strand at 1852.646 kb on + strand at 1852.646 kb on + strand at 1852.651 kb on + strand at 1852.651 kb on + strand at 1852.654 kb on - strand at 1852.654 kb on - strand at 1852.701 kb on + strand at 1852.773 kb on - strand, within Dsui_1764 at 1852.785 kb on + strand, within Dsui_1764 at 1852.785 kb on + strand, within Dsui_1764 at 1852.785 kb on + strand, within Dsui_1764 at 1852.785 kb on + strand, within Dsui_1764 at 1852.785 kb on + strand, within Dsui_1764 at 1852.793 kb on - strand at 1852.793 kb on - strand, within Dsui_1764 at 1852.793 kb on - strand, within Dsui_1764 at 1852.826 kb on - strand, within Dsui_1764 at 1852.847 kb on - strand, within Dsui_1764 at 1852.867 kb on - strand, within Dsui_1764 at 1853.026 kb on - strand, within Dsui_1765 at 1853.069 kb on - strand, within Dsui_1765 at 1853.075 kb on - strand, within Dsui_1765 at 1853.123 kb on + strand, within Dsui_1765 at 1853.131 kb on - strand, within Dsui_1765 at 1853.248 kb on - strand, within Dsui_1765 at 1853.276 kb on + strand, within Dsui_1765 at 1853.286 kb on + strand, within Dsui_1765 at 1853.327 kb on - strand, within Dsui_1765 at 1853.332 kb on - strand, within Dsui_1765 at 1853.392 kb on + strand, within Dsui_1765 at 1853.521 kb on + strand at 1853.522 kb on - strand at 1853.544 kb on - strand, within Dsui_1766 at 1853.624 kb on + strand, within Dsui_1766
Per-strain Table
Position Strand Gene LocusTag Fraction ALP with Fusidic 0.02 mg/ml remove 1,850,052 - Dsui_1761 0.70 -1.2 1,850,052 - Dsui_1761 0.70 +0.6 1,850,052 - Dsui_1761 0.70 +0.6 1,850,082 - Dsui_1761 0.73 -0.4 1,850,090 + Dsui_1761 0.74 -0.4 1,850,094 - Dsui_1761 0.74 -0.4 1,850,118 - Dsui_1761 0.77 -0.9 1,850,216 - Dsui_1761 0.86 +0.6 1,850,314 - -0.4 1,850,319 - +0.6 1,850,410 + -0.4 1,850,415 + +0.1 1,850,436 + +0.0 1,850,465 + Dsui_1762 0.13 +0.9 1,850,522 - Dsui_1762 0.25 -0.4 1,850,530 - Dsui_1762 0.26 +0.6 1,850,536 - Dsui_1762 0.27 +0.6 1,850,592 - Dsui_1762 0.39 +0.6 1,850,595 + Dsui_1762 0.39 -0.4 1,850,618 + Dsui_1762 0.44 +0.9 1,850,651 - Dsui_1762 0.51 +1.6 1,850,701 + Dsui_1762 0.61 -0.7 1,850,756 + Dsui_1762 0.72 +0.7 1,850,757 + Dsui_1762 0.72 +0.6 1,850,758 + Dsui_1762 0.72 -1.4 1,850,758 + Dsui_1762 0.72 -1.5 1,850,764 - +0.6 1,850,766 - Dsui_1762 0.74 -1.9 1,850,781 + Dsui_1762 0.77 -0.9 1,850,815 - Dsui_1762 0.84 -0.9 1,851,000 + -0.7 1,851,151 + Dsui_1763 0.11 +0.0 1,851,276 + Dsui_1763 0.18 +0.2 1,851,285 - Dsui_1763 0.19 +0.6 1,851,312 - Dsui_1763 0.20 -1.4 1,851,326 + Dsui_1763 0.21 +0.4 1,851,479 - Dsui_1763 0.30 -0.4 1,851,530 - Dsui_1763 0.33 -0.9 1,851,542 + Dsui_1763 0.34 +0.5 1,851,568 + Dsui_1763 0.35 +0.9 1,851,630 - Dsui_1763 0.39 +0.6 1,851,687 + Dsui_1763 0.42 -0.1 1,851,709 + Dsui_1763 0.44 +0.2 1,851,811 + Dsui_1763 0.50 +0.7 1,851,852 + Dsui_1763 0.52 -0.1 1,851,922 - Dsui_1763 0.56 -1.4 1,852,009 + Dsui_1763 0.61 +0.3 1,852,009 + Dsui_1763 0.61 -0.2 1,852,017 - Dsui_1763 0.62 -0.9 1,852,035 - Dsui_1763 0.63 +0.6 1,852,167 + Dsui_1763 0.70 +0.8 1,852,175 - Dsui_1763 0.71 -0.4 1,852,272 + Dsui_1763 0.77 -0.8 1,852,327 - +0.6 1,852,377 + Dsui_1763 0.83 -0.1 1,852,551 - +0.6 1,852,646 + +0.6 1,852,646 + +0.4 1,852,651 + -0.4 1,852,651 + +0.4 1,852,654 - -0.4 1,852,654 - -0.9 1,852,701 + -0.9 1,852,773 - Dsui_1764 0.33 -0.4 1,852,785 + Dsui_1764 0.38 +0.1 1,852,785 + Dsui_1764 0.38 -0.2 1,852,785 + Dsui_1764 0.38 -0.4 1,852,785 + Dsui_1764 0.38 +0.5 1,852,785 + Dsui_1764 0.38 +0.1 1,852,793 - +0.6 1,852,793 - Dsui_1764 0.42 -1.4 1,852,793 - Dsui_1764 0.42 -0.9 1,852,826 - Dsui_1764 0.58 +0.6 1,852,847 - Dsui_1764 0.68 -0.4 1,852,867 - Dsui_1764 0.77 -0.4 1,853,026 - Dsui_1765 0.21 -2.5 1,853,069 - Dsui_1765 0.28 +0.6 1,853,075 - Dsui_1765 0.29 +0.6 1,853,123 + Dsui_1765 0.37 +0.2 1,853,131 - Dsui_1765 0.39 -1.4 1,853,248 - Dsui_1765 0.59 -1.7 1,853,276 + Dsui_1765 0.63 +0.0 1,853,286 + Dsui_1765 0.65 +0.3 1,853,327 - Dsui_1765 0.72 +0.6 1,853,332 - Dsui_1765 0.73 +0.6 1,853,392 + Dsui_1765 0.83 +0.6 1,853,521 + +0.1 1,853,522 - -0.0 1,853,544 - Dsui_1766 0.14 -0.9 1,853,624 + Dsui_1766 0.33 -0.3
Or see this region's nucleotide sequence