Experiment: ALP with Fusidic 0.02 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Dsui_1736 and Dsui_1737 are separated by 25 nucleotides Dsui_1737 and Dsui_1738 are separated by 19 nucleotides Dsui_1738 and Dsui_1739 are separated by 22 nucleotides
Dsui_1736: Dsui_1736 - flagellar basal-body rod protein FlgF, at 1,824,913 to 1,825,656
_1736
Dsui_1737: Dsui_1737 - flagellar basal-body rod protein FlgG, at 1,825,682 to 1,826,464
_1737
Dsui_1738: Dsui_1738 - flagellar basal body L-ring protein, at 1,826,484 to 1,827,149
_1738
Dsui_1739: Dsui_1739 - flagellar basal-body P-ring protein, at 1,827,172 to 1,828,305
_1739
Position (kb)
1826
1827
1828 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 1825.511 kb on + strand, within Dsui_1736 at 1825.511 kb on + strand, within Dsui_1736 at 1825.519 kb on - strand, within Dsui_1736 at 1825.617 kb on + strand at 1825.617 kb on + strand at 1825.625 kb on - strand at 1825.708 kb on + strand at 1825.748 kb on + strand at 1825.748 kb on + strand at 1825.801 kb on + strand, within Dsui_1737 at 1825.801 kb on + strand, within Dsui_1737 at 1825.801 kb on + strand, within Dsui_1737 at 1825.848 kb on + strand, within Dsui_1737 at 1825.856 kb on - strand, within Dsui_1737 at 1825.893 kb on + strand, within Dsui_1737 at 1825.963 kb on - strand, within Dsui_1737 at 1826.018 kb on + strand, within Dsui_1737 at 1826.018 kb on + strand, within Dsui_1737 at 1826.018 kb on + strand, within Dsui_1737 at 1826.026 kb on - strand, within Dsui_1737 at 1826.070 kb on - strand at 1826.079 kb on + strand, within Dsui_1737 at 1826.141 kb on - strand, within Dsui_1737 at 1826.183 kb on + strand, within Dsui_1737 at 1826.188 kb on + strand, within Dsui_1737 at 1826.188 kb on + strand, within Dsui_1737 at 1826.188 kb on + strand, within Dsui_1737 at 1826.191 kb on - strand, within Dsui_1737 at 1826.191 kb on - strand, within Dsui_1737 at 1826.196 kb on - strand, within Dsui_1737 at 1826.196 kb on - strand, within Dsui_1737 at 1826.304 kb on - strand, within Dsui_1737 at 1826.305 kb on - strand, within Dsui_1737 at 1826.368 kb on + strand, within Dsui_1737 at 1826.380 kb on + strand, within Dsui_1737 at 1826.381 kb on + strand, within Dsui_1737 at 1826.458 kb on + strand at 1826.461 kb on - strand at 1826.461 kb on - strand at 1826.556 kb on + strand, within Dsui_1738 at 1826.565 kb on - strand, within Dsui_1738 at 1826.567 kb on + strand, within Dsui_1738 at 1826.567 kb on + strand, within Dsui_1738 at 1826.581 kb on + strand, within Dsui_1738 at 1826.581 kb on + strand, within Dsui_1738 at 1826.641 kb on + strand, within Dsui_1738 at 1826.649 kb on - strand, within Dsui_1738 at 1826.743 kb on - strand, within Dsui_1738 at 1826.758 kb on - strand, within Dsui_1738 at 1826.786 kb on - strand, within Dsui_1738 at 1826.896 kb on + strand, within Dsui_1738 at 1826.914 kb on - strand, within Dsui_1738 at 1826.920 kb on - strand, within Dsui_1738 at 1826.970 kb on + strand, within Dsui_1738 at 1826.978 kb on - strand, within Dsui_1738 at 1826.989 kb on + strand, within Dsui_1738 at 1826.997 kb on - strand, within Dsui_1738 at 1826.999 kb on + strand, within Dsui_1738 at 1827.007 kb on - strand, within Dsui_1738 at 1827.007 kb on - strand, within Dsui_1738 at 1827.074 kb on - strand, within Dsui_1738 at 1827.096 kb on + strand at 1827.133 kb on + strand at 1827.141 kb on - strand at 1827.187 kb on + strand at 1827.201 kb on - strand at 1827.201 kb on - strand at 1827.204 kb on + strand at 1827.255 kb on + strand at 1827.289 kb on - strand, within Dsui_1739 at 1827.322 kb on - strand, within Dsui_1739 at 1827.322 kb on - strand, within Dsui_1739 at 1827.338 kb on - strand, within Dsui_1739 at 1827.367 kb on + strand, within Dsui_1739 at 1827.376 kb on + strand, within Dsui_1739 at 1827.489 kb on + strand, within Dsui_1739 at 1827.507 kb on - strand, within Dsui_1739 at 1827.587 kb on - strand at 1827.597 kb on + strand, within Dsui_1739 at 1827.597 kb on + strand, within Dsui_1739 at 1827.597 kb on + strand, within Dsui_1739 at 1827.605 kb on - strand, within Dsui_1739 at 1827.605 kb on - strand, within Dsui_1739 at 1827.610 kb on + strand, within Dsui_1739 at 1827.610 kb on + strand, within Dsui_1739 at 1827.740 kb on + strand, within Dsui_1739 at 1827.798 kb on - strand, within Dsui_1739 at 1827.860 kb on - strand, within Dsui_1739 at 1827.884 kb on + strand, within Dsui_1739 at 1827.989 kb on + strand, within Dsui_1739 at 1828.094 kb on - strand, within Dsui_1739
Per-strain Table
Position Strand Gene LocusTag Fraction ALP with Fusidic 0.02 mg/ml remove 1,825,511 + Dsui_1736 0.80 -0.4 1,825,511 + Dsui_1736 0.80 -2.0 1,825,519 - Dsui_1736 0.81 +0.6 1,825,617 + -1.0 1,825,617 + -1.7 1,825,625 - +0.6 1,825,708 + -2.2 1,825,748 + -1.7 1,825,748 + -2.7 1,825,801 + Dsui_1737 0.15 -2.5 1,825,801 + Dsui_1737 0.15 +1.2 1,825,801 + Dsui_1737 0.15 -1.7 1,825,848 + Dsui_1737 0.21 -2.2 1,825,856 - Dsui_1737 0.22 +0.6 1,825,893 + Dsui_1737 0.27 -0.4 1,825,963 - Dsui_1737 0.36 -1.4 1,826,018 + Dsui_1737 0.43 +0.6 1,826,018 + Dsui_1737 0.43 +1.6 1,826,018 + Dsui_1737 0.43 -1.3 1,826,026 - Dsui_1737 0.44 +0.5 1,826,070 - -1.4 1,826,079 + Dsui_1737 0.51 +0.0 1,826,141 - Dsui_1737 0.59 -0.4 1,826,183 + Dsui_1737 0.64 -1.7 1,826,188 + Dsui_1737 0.65 +0.6 1,826,188 + Dsui_1737 0.65 +0.6 1,826,188 + Dsui_1737 0.65 -0.4 1,826,191 - Dsui_1737 0.65 +0.6 1,826,191 - Dsui_1737 0.65 -0.4 1,826,196 - Dsui_1737 0.66 -0.4 1,826,196 - Dsui_1737 0.66 -1.7 1,826,304 - Dsui_1737 0.79 -0.4 1,826,305 - Dsui_1737 0.80 -0.4 1,826,368 + Dsui_1737 0.88 -0.1 1,826,380 + Dsui_1737 0.89 -1.4 1,826,381 + Dsui_1737 0.89 +0.6 1,826,458 + +0.6 1,826,461 - -2.0 1,826,461 - +0.6 1,826,556 + Dsui_1738 0.11 -1.0 1,826,565 - Dsui_1738 0.12 -0.4 1,826,567 + Dsui_1738 0.12 -1.0 1,826,567 + Dsui_1738 0.12 +0.6 1,826,581 + Dsui_1738 0.15 -2.2 1,826,581 + Dsui_1738 0.15 +0.6 1,826,641 + Dsui_1738 0.24 -1.0 1,826,649 - Dsui_1738 0.25 +0.6 1,826,743 - Dsui_1738 0.39 -0.4 1,826,758 - Dsui_1738 0.41 -0.4 1,826,786 - Dsui_1738 0.45 +0.6 1,826,896 + Dsui_1738 0.62 -0.4 1,826,914 - Dsui_1738 0.65 +0.6 1,826,920 - Dsui_1738 0.65 +0.6 1,826,970 + Dsui_1738 0.73 -1.0 1,826,978 - Dsui_1738 0.74 +0.6 1,826,989 + Dsui_1738 0.76 +0.6 1,826,997 - Dsui_1738 0.77 +0.6 1,826,999 + Dsui_1738 0.77 -1.4 1,827,007 - Dsui_1738 0.79 +0.6 1,827,007 - Dsui_1738 0.79 +0.6 1,827,074 - Dsui_1738 0.89 -0.4 1,827,096 + +1.6 1,827,133 + -1.0 1,827,141 - -1.4 1,827,187 + -3.1 1,827,201 - +0.6 1,827,201 - +0.6 1,827,204 + -0.4 1,827,255 + +0.6 1,827,289 - Dsui_1739 0.10 -0.4 1,827,322 - Dsui_1739 0.13 -1.0 1,827,322 - Dsui_1739 0.13 -0.4 1,827,338 - Dsui_1739 0.15 -1.0 1,827,367 + Dsui_1739 0.17 -1.7 1,827,376 + Dsui_1739 0.18 -2.2 1,827,489 + Dsui_1739 0.28 -0.4 1,827,507 - Dsui_1739 0.30 -1.0 1,827,587 - +0.6 1,827,597 + Dsui_1739 0.37 -0.4 1,827,597 + Dsui_1739 0.37 -0.4 1,827,597 + Dsui_1739 0.37 -2.0 1,827,605 - Dsui_1739 0.38 +0.6 1,827,605 - Dsui_1739 0.38 -1.0 1,827,610 + Dsui_1739 0.39 -1.4 1,827,610 + Dsui_1739 0.39 -1.7 1,827,740 + Dsui_1739 0.50 +0.6 1,827,798 - Dsui_1739 0.55 -1.0 1,827,860 - Dsui_1739 0.61 -1.4 1,827,884 + Dsui_1739 0.63 -0.4 1,827,989 + Dsui_1739 0.72 -0.4 1,828,094 - Dsui_1739 0.81 +0.6
Or see this region's nucleotide sequence