Experiment: ALP with Fusidic 0.02 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Dsui_1579 and Dsui_1580 are separated by 186 nucleotides Dsui_1580 and Dsui_1581 are separated by 4 nucleotides Dsui_1581 and Dsui_1582 are separated by 186 nucleotides
Dsui_1579: Dsui_1579 - ABC-type branched-chain amino acid transport system, periplasmic component, at 1,655,149 to 1,656,357
_1579
Dsui_1580: Dsui_1580 - PAS domain S-box, at 1,656,544 to 1,659,249
_1580
Dsui_1581: Dsui_1581 - response regulator, at 1,659,254 to 1,659,904
_1581
Dsui_1582: Dsui_1582 - Fe-S oxidoreductase, at 1,660,091 to 1,660,819
_1582
Position (kb)
1656
1657
1658
1659
1660 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 1655.705 kb on + strand at 1655.722 kb on - strand, within Dsui_1579 at 1655.732 kb on - strand, within Dsui_1579 at 1655.916 kb on - strand, within Dsui_1579 at 1656.019 kb on - strand, within Dsui_1579 at 1656.058 kb on + strand, within Dsui_1579 at 1656.158 kb on + strand at 1656.204 kb on + strand, within Dsui_1579 at 1656.208 kb on + strand at 1656.279 kb on + strand at 1656.297 kb on - strand at 1656.359 kb on + strand at 1656.377 kb on + strand at 1656.442 kb on - strand at 1656.552 kb on - strand at 1656.552 kb on - strand at 1656.614 kb on - strand at 1656.625 kb on - strand at 1656.653 kb on + strand at 1656.841 kb on + strand, within Dsui_1580 at 1656.891 kb on - strand at 1656.953 kb on + strand, within Dsui_1580 at 1656.961 kb on - strand at 1656.961 kb on - strand, within Dsui_1580 at 1656.981 kb on + strand, within Dsui_1580 at 1657.049 kb on + strand, within Dsui_1580 at 1657.219 kb on - strand at 1657.320 kb on + strand, within Dsui_1580 at 1657.360 kb on - strand at 1657.519 kb on + strand, within Dsui_1580 at 1657.719 kb on + strand, within Dsui_1580 at 1657.719 kb on + strand, within Dsui_1580 at 1657.727 kb on - strand at 1657.739 kb on + strand, within Dsui_1580 at 1657.740 kb on - strand, within Dsui_1580 at 1657.745 kb on - strand at 1657.799 kb on - strand, within Dsui_1580 at 1657.932 kb on - strand, within Dsui_1580 at 1657.935 kb on - strand, within Dsui_1580 at 1658.150 kb on - strand, within Dsui_1580 at 1658.185 kb on + strand, within Dsui_1580 at 1658.211 kb on + strand, within Dsui_1580 at 1658.211 kb on + strand, within Dsui_1580 at 1658.290 kb on + strand, within Dsui_1580 at 1658.308 kb on - strand, within Dsui_1580 at 1658.496 kb on + strand, within Dsui_1580 at 1658.648 kb on - strand, within Dsui_1580 at 1658.827 kb on + strand, within Dsui_1580 at 1658.874 kb on - strand, within Dsui_1580 at 1658.927 kb on - strand, within Dsui_1580 at 1659.009 kb on + strand at 1659.043 kb on - strand at 1659.072 kb on + strand at 1659.080 kb on - strand at 1659.113 kb on - strand at 1659.229 kb on + strand at 1659.263 kb on + strand at 1659.268 kb on - strand at 1659.291 kb on + strand at 1659.401 kb on - strand at 1659.590 kb on - strand, within Dsui_1581 at 1659.602 kb on + strand, within Dsui_1581 at 1659.610 kb on - strand at 1659.804 kb on + strand, within Dsui_1581 at 1659.873 kb on - strand at 1659.907 kb on + strand at 1659.907 kb on - strand at 1660.143 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction ALP with Fusidic 0.02 mg/ml remove 1,655,705 + +0.6 1,655,722 - Dsui_1579 0.47 +0.9 1,655,732 - Dsui_1579 0.48 +2.6 1,655,916 - Dsui_1579 0.63 -2.4 1,656,019 - Dsui_1579 0.72 -0.4 1,656,058 + Dsui_1579 0.75 -2.3 1,656,158 + +0.6 1,656,204 + Dsui_1579 0.87 -1.0 1,656,208 + -0.4 1,656,279 + +0.6 1,656,297 - -1.0 1,656,359 + -0.4 1,656,377 + +0.6 1,656,442 - -0.4 1,656,552 - -0.4 1,656,552 - -0.4 1,656,614 - +0.6 1,656,625 - -1.0 1,656,653 + -1.0 1,656,841 + Dsui_1580 0.11 -1.1 1,656,891 - +0.6 1,656,953 + Dsui_1580 0.15 -1.7 1,656,961 - +0.6 1,656,961 - Dsui_1580 0.15 -2.0 1,656,981 + Dsui_1580 0.16 -1.1 1,657,049 + Dsui_1580 0.19 -1.1 1,657,219 - -1.0 1,657,320 + Dsui_1580 0.29 -1.0 1,657,360 - -0.4 1,657,519 + Dsui_1580 0.36 -1.1 1,657,719 + Dsui_1580 0.43 -1.6 1,657,719 + Dsui_1580 0.43 -0.4 1,657,727 - -1.0 1,657,739 + Dsui_1580 0.44 -0.9 1,657,740 - Dsui_1580 0.44 -1.0 1,657,745 - -0.4 1,657,799 - Dsui_1580 0.46 +0.6 1,657,932 - Dsui_1580 0.51 -0.4 1,657,935 - Dsui_1580 0.51 -1.0 1,658,150 - Dsui_1580 0.59 +0.6 1,658,185 + Dsui_1580 0.61 -1.4 1,658,211 + Dsui_1580 0.62 -3.7 1,658,211 + Dsui_1580 0.62 -2.7 1,658,290 + Dsui_1580 0.65 -1.7 1,658,308 - Dsui_1580 0.65 -0.4 1,658,496 + Dsui_1580 0.72 -3.5 1,658,648 - Dsui_1580 0.78 -0.4 1,658,827 + Dsui_1580 0.84 -2.5 1,658,874 - Dsui_1580 0.86 +0.6 1,658,927 - Dsui_1580 0.88 -0.4 1,659,009 + -1.4 1,659,043 - -1.8 1,659,072 + -2.0 1,659,080 - -0.8 1,659,113 - +0.6 1,659,229 + -1.4 1,659,263 + +1.5 1,659,268 - -1.4 1,659,291 + +0.6 1,659,401 - +0.6 1,659,590 - Dsui_1581 0.52 -1.4 1,659,602 + Dsui_1581 0.53 +0.2 1,659,610 - +0.6 1,659,804 + Dsui_1581 0.84 +0.2 1,659,873 - -0.4 1,659,907 + +0.4 1,659,907 - -1.0 1,660,143 - -1.4
Or see this region's nucleotide sequence