Experiment: ALP with Fusidic 0.02 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Dsui_1547 and Dsui_1548 are separated by 67 nucleotides Dsui_1548 and Dsui_1549 are separated by 25 nucleotides Dsui_1549 and Dsui_1550 are separated by 7 nucleotides
Dsui_1547: Dsui_1547 - putative membrane-associated protein, at 1,607,304 to 1,607,966
_1547
Dsui_1548: Dsui_1548 - response regulator with CheY-like receiver domain and winged-helix DNA-binding domain, at 1,608,034 to 1,608,480
_1548
Dsui_1549: Dsui_1549 - PAS domain S-box, at 1,608,506 to 1,611,505
_1549
Dsui_1550: Dsui_1550 - diguanylate cyclase (GGDEF) domain-containing protein, at 1,611,513 to 1,613,231
_1550
Position (kb)
1608
1609
1610
1611
1612 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 1607.986 kb on - strand at 1608.008 kb on - strand at 1608.008 kb on - strand at 1608.008 kb on - strand at 1608.037 kb on + strand at 1608.074 kb on + strand at 1608.074 kb on + strand at 1608.074 kb on + strand at 1608.082 kb on - strand, within Dsui_1548 at 1608.084 kb on + strand, within Dsui_1548 at 1608.174 kb on + strand at 1608.186 kb on + strand at 1608.255 kb on + strand at 1608.263 kb on - strand at 1608.305 kb on + strand, within Dsui_1548 at 1608.342 kb on + strand, within Dsui_1548 at 1608.353 kb on + strand at 1608.513 kb on + strand at 1608.531 kb on - strand at 1608.577 kb on - strand at 1609.074 kb on + strand at 1609.150 kb on + strand, within Dsui_1549 at 1609.172 kb on - strand, within Dsui_1549 at 1609.384 kb on - strand, within Dsui_1549 at 1609.495 kb on + strand, within Dsui_1549 at 1609.503 kb on - strand at 1609.503 kb on - strand, within Dsui_1549 at 1609.694 kb on - strand, within Dsui_1549 at 1609.775 kb on - strand, within Dsui_1549 at 1609.804 kb on - strand, within Dsui_1549 at 1609.855 kb on + strand, within Dsui_1549 at 1609.942 kb on + strand, within Dsui_1549 at 1609.975 kb on - strand, within Dsui_1549 at 1609.975 kb on - strand, within Dsui_1549 at 1610.075 kb on - strand, within Dsui_1549 at 1610.080 kb on + strand, within Dsui_1549 at 1610.080 kb on + strand, within Dsui_1549 at 1610.102 kb on + strand, within Dsui_1549 at 1610.144 kb on - strand, within Dsui_1549 at 1610.171 kb on - strand, within Dsui_1549 at 1610.213 kb on + strand at 1610.266 kb on + strand, within Dsui_1549 at 1610.349 kb on + strand at 1610.357 kb on - strand, within Dsui_1549 at 1610.367 kb on - strand, within Dsui_1549 at 1610.381 kb on + strand, within Dsui_1549 at 1610.491 kb on + strand at 1610.734 kb on + strand, within Dsui_1549 at 1610.750 kb on + strand, within Dsui_1549 at 1610.752 kb on + strand at 1610.792 kb on + strand at 1610.838 kb on - strand, within Dsui_1549 at 1611.012 kb on + strand, within Dsui_1549 at 1611.022 kb on + strand, within Dsui_1549 at 1611.030 kb on - strand at 1611.087 kb on + strand at 1611.095 kb on - strand, within Dsui_1549 at 1611.128 kb on + strand at 1611.149 kb on - strand, within Dsui_1549 at 1611.372 kb on - strand at 1611.538 kb on - strand at 1611.620 kb on + strand at 1611.665 kb on - strand at 1611.850 kb on - strand, within Dsui_1550 at 1611.852 kb on + strand at 1611.860 kb on - strand, within Dsui_1550 at 1611.860 kb on - strand, within Dsui_1550 at 1611.990 kb on + strand, within Dsui_1550 at 1612.059 kb on + strand, within Dsui_1550 at 1612.199 kb on + strand, within Dsui_1550 at 1612.207 kb on - strand at 1612.218 kb on + strand at 1612.247 kb on - strand, within Dsui_1550 at 1612.301 kb on - strand, within Dsui_1550 at 1612.325 kb on - strand, within Dsui_1550 at 1612.385 kb on - strand, within Dsui_1550 at 1612.456 kb on - strand, within Dsui_1550 at 1612.457 kb on - strand, within Dsui_1550
Per-strain Table
Position Strand Gene LocusTag Fraction ALP with Fusidic 0.02 mg/ml remove 1,607,986 - -1.8 1,608,008 - +1.4 1,608,008 - +0.6 1,608,008 - -1.6 1,608,037 + -0.4 1,608,074 + -1.8 1,608,074 + +0.6 1,608,074 + -1.8 1,608,082 - Dsui_1548 0.11 +0.6 1,608,084 + Dsui_1548 0.11 -1.0 1,608,174 + +0.6 1,608,186 + -0.4 1,608,255 + +0.6 1,608,263 - +0.6 1,608,305 + Dsui_1548 0.61 -1.0 1,608,342 + Dsui_1548 0.69 +0.6 1,608,353 + -0.4 1,608,513 + +0.5 1,608,531 - +0.5 1,608,577 - -0.5 1,609,074 + +0.5 1,609,150 + Dsui_1549 0.21 -0.5 1,609,172 - Dsui_1549 0.22 +0.5 1,609,384 - Dsui_1549 0.29 -1.0 1,609,495 + Dsui_1549 0.33 -1.5 1,609,503 - +0.5 1,609,503 - Dsui_1549 0.33 -1.0 1,609,694 - Dsui_1549 0.40 -1.0 1,609,775 - Dsui_1549 0.42 -0.5 1,609,804 - Dsui_1549 0.43 +0.5 1,609,855 + Dsui_1549 0.45 -2.5 1,609,942 + Dsui_1549 0.48 +0.5 1,609,975 - Dsui_1549 0.49 +0.5 1,609,975 - Dsui_1549 0.49 +0.5 1,610,075 - Dsui_1549 0.52 +0.5 1,610,080 + Dsui_1549 0.52 -1.5 1,610,080 + Dsui_1549 0.52 -2.3 1,610,102 + Dsui_1549 0.53 -2.5 1,610,144 - Dsui_1549 0.55 +0.5 1,610,171 - Dsui_1549 0.56 +0.5 1,610,213 + +0.5 1,610,266 + Dsui_1549 0.59 +0.5 1,610,349 + -0.5 1,610,357 - Dsui_1549 0.62 +0.5 1,610,367 - Dsui_1549 0.62 -1.8 1,610,381 + Dsui_1549 0.62 -2.0 1,610,491 + +0.5 1,610,734 + Dsui_1549 0.74 -1.5 1,610,750 + Dsui_1549 0.75 +0.5 1,610,752 + +0.5 1,610,792 + +0.5 1,610,838 - Dsui_1549 0.78 -0.5 1,611,012 + Dsui_1549 0.84 -1.5 1,611,022 + Dsui_1549 0.84 -1.0 1,611,030 - +0.5 1,611,087 + -0.5 1,611,095 - Dsui_1549 0.86 +0.5 1,611,128 + +0.5 1,611,149 - Dsui_1549 0.88 -1.5 1,611,372 - -0.0 1,611,538 - -1.8 1,611,620 + +0.6 1,611,665 - +0.6 1,611,850 - Dsui_1550 0.20 -3.4 1,611,852 + +0.6 1,611,860 - Dsui_1550 0.20 -1.0 1,611,860 - Dsui_1550 0.20 +0.6 1,611,990 + Dsui_1550 0.28 -1.0 1,612,059 + Dsui_1550 0.32 -1.4 1,612,199 + Dsui_1550 0.40 -1.0 1,612,207 - +0.6 1,612,218 + +0.6 1,612,247 - Dsui_1550 0.43 +1.0 1,612,301 - Dsui_1550 0.46 -1.4 1,612,325 - Dsui_1550 0.47 -0.4 1,612,385 - Dsui_1550 0.51 +0.6 1,612,456 - Dsui_1550 0.55 -0.4 1,612,457 - Dsui_1550 0.55 -1.4
Or see this region's nucleotide sequence