Experiment: ALP with Fusidic 0.02 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Dsui_1518 and Dsui_1519 are separated by 33 nucleotides Dsui_1519 and Dsui_1520 are separated by 86 nucleotides Dsui_1520 and Dsui_1521 are separated by 72 nucleotides Dsui_1521 and Dsui_1522 are separated by 14 nucleotides
Dsui_1518: Dsui_1518 - putative permease, at 1,580,859 to 1,581,980
_1518
Dsui_1519: Dsui_1519 - phosphoribosylaminoimidazole synthetase, at 1,582,014 to 1,583,063
_1519
Dsui_1520: Dsui_1520 - cytochrome c551/c552, at 1,583,150 to 1,583,467
_1520
Dsui_1521: Dsui_1521 - hypothetical protein, at 1,583,540 to 1,583,995
_1521
Dsui_1522: Dsui_1522 - serine/threonine protein kinase, at 1,584,010 to 1,585,344
_1522
Position (kb)
1582
1583
1584 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 1581.051 kb on - strand, within Dsui_1518 at 1581.150 kb on + strand, within Dsui_1518 at 1581.202 kb on - strand, within Dsui_1518 at 1581.204 kb on - strand, within Dsui_1518 at 1581.245 kb on + strand, within Dsui_1518 at 1581.245 kb on + strand, within Dsui_1518 at 1581.253 kb on - strand, within Dsui_1518 at 1581.259 kb on - strand, within Dsui_1518 at 1581.341 kb on + strand, within Dsui_1518 at 1581.394 kb on - strand, within Dsui_1518 at 1581.464 kb on + strand at 1581.632 kb on - strand, within Dsui_1518 at 1581.910 kb on - strand at 1583.062 kb on - strand at 1583.084 kb on + strand at 1583.087 kb on - strand at 1583.150 kb on - strand at 1583.150 kb on - strand at 1583.182 kb on + strand, within Dsui_1520 at 1583.241 kb on - strand, within Dsui_1520 at 1583.241 kb on - strand, within Dsui_1520 at 1583.261 kb on + strand, within Dsui_1520 at 1583.271 kb on + strand, within Dsui_1520 at 1583.274 kb on - strand, within Dsui_1520 at 1583.291 kb on + strand, within Dsui_1520 at 1583.314 kb on - strand, within Dsui_1520 at 1583.426 kb on + strand, within Dsui_1520 at 1583.438 kb on - strand at 1583.469 kb on - strand at 1583.485 kb on - strand at 1583.485 kb on - strand at 1583.645 kb on + strand, within Dsui_1521 at 1583.675 kb on + strand at 1583.708 kb on + strand, within Dsui_1521 at 1583.732 kb on - strand, within Dsui_1521 at 1583.785 kb on - strand, within Dsui_1521 at 1583.974 kb on + strand at 1584.001 kb on - strand at 1584.030 kb on + strand at 1584.062 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction ALP with Fusidic 0.02 mg/ml remove 1,581,051 - Dsui_1518 0.17 -0.3 1,581,150 + Dsui_1518 0.26 -1.4 1,581,202 - Dsui_1518 0.31 -0.3 1,581,204 - Dsui_1518 0.31 -0.0 1,581,245 + Dsui_1518 0.34 +0.6 1,581,245 + Dsui_1518 0.34 +0.6 1,581,253 - Dsui_1518 0.35 -0.2 1,581,259 - Dsui_1518 0.36 -0.3 1,581,341 + Dsui_1518 0.43 -1.0 1,581,394 - Dsui_1518 0.48 -0.1 1,581,464 + +0.6 1,581,632 - Dsui_1518 0.69 -0.1 1,581,910 - +0.0 1,583,062 - -1.8 1,583,084 + +0.4 1,583,087 - -1.9 1,583,150 - -1.0 1,583,150 - -0.4 1,583,182 + Dsui_1520 0.10 +0.2 1,583,241 - Dsui_1520 0.29 -2.0 1,583,241 - Dsui_1520 0.29 +0.6 1,583,261 + Dsui_1520 0.35 -0.7 1,583,271 + Dsui_1520 0.38 -0.5 1,583,274 - Dsui_1520 0.39 +0.6 1,583,291 + Dsui_1520 0.44 -0.5 1,583,314 - Dsui_1520 0.52 +0.6 1,583,426 + Dsui_1520 0.87 -0.4 1,583,438 - +2.1 1,583,469 - -1.8 1,583,485 - +1.6 1,583,485 - +2.6 1,583,645 + Dsui_1521 0.23 +0.6 1,583,675 + +1.6 1,583,708 + Dsui_1521 0.37 -3.4 1,583,732 - Dsui_1521 0.42 +0.4 1,583,785 - Dsui_1521 0.54 -0.2 1,583,974 + -0.4 1,584,001 - -0.1 1,584,030 + +0.4 1,584,062 - -0.1
Or see this region's nucleotide sequence