Experiment: ALP with Fusidic 0.02 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Dsui_1501 and Dsui_1502 are separated by 183 nucleotides Dsui_1502 and Dsui_1503 overlap by 23 nucleotides Dsui_1503 and Dsui_1504 are separated by 24 nucleotides
Dsui_1501: Dsui_1501 - PAS domain S-box/diguanylate cyclase (GGDEF) domain-containing protein, at 1,564,716 to 1,567,523
_1501
Dsui_1502: Dsui_1502 - putative membrane protein, at 1,567,707 to 1,568,207
_1502
Dsui_1503: Dsui_1503 - Zn-dependent protease with chaperone function, at 1,568,185 to 1,569,150
_1503
Dsui_1504: Dsui_1504 - trypsin-like serine protease with C-terminal PDZ domain, at 1,569,175 to 1,570,326
_1504
Position (kb)
1568
1569
1570 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 1567.193 kb on - strand, within Dsui_1501 at 1567.302 kb on + strand at 1567.302 kb on + strand at 1567.405 kb on + strand at 1567.450 kb on - strand at 1567.535 kb on + strand at 1567.542 kb on + strand at 1567.560 kb on - strand at 1567.802 kb on - strand, within Dsui_1502 at 1567.816 kb on - strand, within Dsui_1502 at 1567.885 kb on - strand, within Dsui_1502 at 1567.895 kb on - strand, within Dsui_1502 at 1567.994 kb on - strand, within Dsui_1502 at 1568.002 kb on + strand, within Dsui_1502 at 1568.012 kb on + strand, within Dsui_1502 at 1568.020 kb on + strand, within Dsui_1502 at 1568.020 kb on - strand, within Dsui_1502 at 1568.033 kb on - strand, within Dsui_1502 at 1568.079 kb on - strand, within Dsui_1502 at 1568.411 kb on + strand, within Dsui_1503 at 1568.524 kb on - strand, within Dsui_1503 at 1568.579 kb on - strand, within Dsui_1503 at 1568.699 kb on - strand, within Dsui_1503 at 1568.757 kb on - strand, within Dsui_1503 at 1568.908 kb on - strand, within Dsui_1503 at 1568.965 kb on + strand, within Dsui_1503 at 1569.165 kb on - strand at 1569.171 kb on + strand at 1569.177 kb on + strand at 1569.182 kb on + strand at 1569.182 kb on + strand at 1569.182 kb on + strand at 1569.183 kb on + strand at 1569.223 kb on - strand at 1569.371 kb on + strand, within Dsui_1504 at 1569.371 kb on + strand, within Dsui_1504 at 1569.371 kb on + strand, within Dsui_1504 at 1569.379 kb on - strand, within Dsui_1504 at 1569.413 kb on - strand, within Dsui_1504 at 1569.554 kb on + strand, within Dsui_1504 at 1569.587 kb on - strand, within Dsui_1504 at 1569.594 kb on + strand, within Dsui_1504 at 1569.602 kb on - strand, within Dsui_1504 at 1569.723 kb on + strand, within Dsui_1504 at 1569.725 kb on + strand, within Dsui_1504 at 1569.862 kb on + strand, within Dsui_1504 at 1570.030 kb on - strand, within Dsui_1504 at 1570.075 kb on - strand, within Dsui_1504
Per-strain Table
Position Strand Gene LocusTag Fraction ALP with Fusidic 0.02 mg/ml remove 1,567,193 - Dsui_1501 0.88 +0.5 1,567,302 + +2.5 1,567,302 + +1.8 1,567,405 + +0.4 1,567,450 - +0.5 1,567,535 + -0.5 1,567,542 + -1.4 1,567,560 - -0.7 1,567,802 - Dsui_1502 0.19 +0.5 1,567,816 - Dsui_1502 0.22 +0.5 1,567,885 - Dsui_1502 0.36 +0.5 1,567,895 - Dsui_1502 0.38 -0.2 1,567,994 - Dsui_1502 0.57 -1.0 1,568,002 + Dsui_1502 0.59 +0.8 1,568,012 + Dsui_1502 0.61 -0.9 1,568,020 + Dsui_1502 0.62 -1.0 1,568,020 - Dsui_1502 0.62 -1.8 1,568,033 - Dsui_1502 0.65 -2.3 1,568,079 - Dsui_1502 0.74 -0.5 1,568,411 + Dsui_1503 0.23 -2.0 1,568,524 - Dsui_1503 0.35 -0.8 1,568,579 - Dsui_1503 0.41 +0.5 1,568,699 - Dsui_1503 0.53 -0.5 1,568,757 - Dsui_1503 0.59 +0.5 1,568,908 - Dsui_1503 0.75 -2.0 1,568,965 + Dsui_1503 0.81 -0.2 1,569,165 - -1.0 1,569,171 + +0.1 1,569,177 + +0.1 1,569,182 + +0.8 1,569,182 + -1.5 1,569,182 + -1.6 1,569,183 + -1.0 1,569,223 - -1.8 1,569,371 + Dsui_1504 0.17 +0.2 1,569,371 + Dsui_1504 0.17 +0.1 1,569,371 + Dsui_1504 0.17 -0.2 1,569,379 - Dsui_1504 0.18 -2.3 1,569,413 - Dsui_1504 0.21 +0.5 1,569,554 + Dsui_1504 0.33 -1.8 1,569,587 - Dsui_1504 0.36 -1.0 1,569,594 + Dsui_1504 0.36 -0.2 1,569,602 - Dsui_1504 0.37 -0.5 1,569,723 + Dsui_1504 0.48 -0.3 1,569,725 + Dsui_1504 0.48 -0.3 1,569,862 + Dsui_1504 0.60 +1.3 1,570,030 - Dsui_1504 0.74 +0.5 1,570,075 - Dsui_1504 0.78 -1.0
Or see this region's nucleotide sequence