Experiment: ALP with Fusidic 0.02 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Dsui_1430 and Dsui_1431 overlap by 1 nucleotides Dsui_1431 and Dsui_1432 are separated by 43 nucleotides Dsui_1432 and Dsui_1433 are separated by 8 nucleotides Dsui_1433 and Dsui_1434 overlap by 1 nucleotides
Dsui_1430: Dsui_1430 - PAS domain S-box/diguanylate cyclase (GGDEF) domain-containing protein, at 1,484,555 to 1,485,541
_1430
Dsui_1431: Dsui_1431 - chemotaxis protein CheC, inhibitor of MCP methylation, at 1,485,541 to 1,486,158
_1431
Dsui_1432: Dsui_1432 - chemotaxis response regulator containing a CheY-like receiver domain and a methylesterase domain, at 1,486,202 to 1,487,329
_1432
Dsui_1433: Dsui_1433 - chemotaxis protein, at 1,487,338 to 1,487,835
_1433
Dsui_1434: Dsui_1434 - methylase of chemotaxis methyl-accepting protein, at 1,487,835 to 1,488,647
_1434
Position (kb)
1486
1487
1488 Strain fitness (log2 ratio)
-2
-1
0
1 at 1485.216 kb on + strand, within Dsui_1430 at 1485.248 kb on + strand, within Dsui_1430 at 1485.289 kb on - strand, within Dsui_1430 at 1485.344 kb on - strand, within Dsui_1430 at 1485.365 kb on - strand, within Dsui_1430 at 1485.420 kb on - strand, within Dsui_1430 at 1485.450 kb on + strand at 1485.486 kb on - strand at 1485.559 kb on - strand at 1485.559 kb on - strand at 1485.559 kb on - strand at 1485.559 kb on - strand at 1485.559 kb on - strand at 1485.584 kb on + strand at 1485.648 kb on + strand, within Dsui_1431 at 1485.668 kb on - strand, within Dsui_1431 at 1485.695 kb on - strand, within Dsui_1431 at 1485.738 kb on - strand, within Dsui_1431 at 1485.743 kb on - strand, within Dsui_1431 at 1485.743 kb on - strand, within Dsui_1431 at 1485.808 kb on - strand, within Dsui_1431 at 1485.813 kb on - strand, within Dsui_1431 at 1485.840 kb on + strand, within Dsui_1431 at 1485.840 kb on + strand, within Dsui_1431 at 1485.866 kb on - strand, within Dsui_1431 at 1485.934 kb on - strand, within Dsui_1431 at 1485.939 kb on - strand, within Dsui_1431 at 1485.971 kb on + strand, within Dsui_1431 at 1486.008 kb on + strand, within Dsui_1431 at 1486.013 kb on - strand, within Dsui_1431 at 1486.032 kb on + strand, within Dsui_1431 at 1486.096 kb on - strand, within Dsui_1431 at 1486.119 kb on - strand at 1486.165 kb on + strand at 1486.183 kb on - strand at 1486.205 kb on + strand at 1486.295 kb on + strand at 1486.313 kb on - strand at 1486.322 kb on - strand, within Dsui_1432 at 1486.445 kb on - strand, within Dsui_1432 at 1486.472 kb on + strand, within Dsui_1432 at 1486.485 kb on - strand, within Dsui_1432 at 1486.503 kb on + strand, within Dsui_1432 at 1486.513 kb on - strand, within Dsui_1432 at 1486.620 kb on + strand, within Dsui_1432 at 1487.035 kb on + strand, within Dsui_1432 at 1487.077 kb on + strand, within Dsui_1432 at 1487.077 kb on + strand, within Dsui_1432 at 1487.120 kb on - strand, within Dsui_1432 at 1487.194 kb on - strand, within Dsui_1432 at 1487.216 kb on - strand, within Dsui_1432 at 1487.226 kb on - strand at 1487.350 kb on + strand at 1487.423 kb on + strand, within Dsui_1433 at 1487.431 kb on + strand, within Dsui_1433 at 1487.866 kb on + strand at 1487.874 kb on - strand at 1487.888 kb on + strand at 1487.988 kb on + strand, within Dsui_1434 at 1487.988 kb on + strand, within Dsui_1434 at 1487.991 kb on - strand, within Dsui_1434 at 1488.030 kb on - strand, within Dsui_1434 at 1488.207 kb on - strand, within Dsui_1434 at 1488.286 kb on + strand, within Dsui_1434 at 1488.286 kb on + strand, within Dsui_1434
Per-strain Table
Position Strand Gene LocusTag Fraction ALP with Fusidic 0.02 mg/ml remove 1,485,216 + Dsui_1430 0.67 -0.5 1,485,248 + Dsui_1430 0.70 -2.7 1,485,289 - Dsui_1430 0.74 -0.5 1,485,344 - Dsui_1430 0.80 -0.2 1,485,365 - Dsui_1430 0.82 -0.4 1,485,420 - Dsui_1430 0.88 -0.1 1,485,450 + +0.5 1,485,486 - +0.1 1,485,559 - +0.6 1,485,559 - -0.2 1,485,559 - +0.7 1,485,559 - -0.2 1,485,559 - +0.1 1,485,584 + -1.8 1,485,648 + Dsui_1431 0.17 -1.8 1,485,668 - Dsui_1431 0.21 +0.0 1,485,695 - Dsui_1431 0.25 -0.0 1,485,738 - Dsui_1431 0.32 +0.2 1,485,743 - Dsui_1431 0.33 +0.2 1,485,743 - Dsui_1431 0.33 -0.2 1,485,808 - Dsui_1431 0.43 -0.2 1,485,813 - Dsui_1431 0.44 +0.1 1,485,840 + Dsui_1431 0.48 -1.5 1,485,840 + Dsui_1431 0.48 -1.1 1,485,866 - Dsui_1431 0.53 +1.0 1,485,934 - Dsui_1431 0.64 -0.2 1,485,939 - Dsui_1431 0.64 +0.0 1,485,971 + Dsui_1431 0.70 -1.5 1,486,008 + Dsui_1431 0.76 +0.5 1,486,013 - Dsui_1431 0.76 +0.1 1,486,032 + Dsui_1431 0.79 +0.5 1,486,096 - Dsui_1431 0.90 +0.7 1,486,119 - -0.3 1,486,165 + -1.1 1,486,183 - -0.1 1,486,205 + -1.5 1,486,295 + +0.5 1,486,313 - +0.0 1,486,322 - Dsui_1432 0.11 -0.2 1,486,445 - Dsui_1432 0.22 -0.2 1,486,472 + Dsui_1432 0.24 +0.5 1,486,485 - Dsui_1432 0.25 -0.6 1,486,503 + Dsui_1432 0.27 +0.2 1,486,513 - Dsui_1432 0.28 -0.3 1,486,620 + Dsui_1432 0.37 -2.5 1,487,035 + Dsui_1432 0.74 -0.5 1,487,077 + Dsui_1432 0.78 -1.5 1,487,077 + Dsui_1432 0.78 -1.8 1,487,120 - Dsui_1432 0.81 -0.5 1,487,194 - Dsui_1432 0.88 -0.1 1,487,216 - Dsui_1432 0.90 -0.1 1,487,226 - -0.5 1,487,350 + +0.5 1,487,423 + Dsui_1433 0.17 -1.5 1,487,431 + Dsui_1433 0.19 -1.8 1,487,866 + -0.8 1,487,874 - -0.2 1,487,888 + -0.5 1,487,988 + Dsui_1434 0.19 +0.5 1,487,988 + Dsui_1434 0.19 +0.5 1,487,991 - Dsui_1434 0.19 -0.2 1,488,030 - Dsui_1434 0.24 +0.1 1,488,207 - Dsui_1434 0.46 -0.7 1,488,286 + Dsui_1434 0.55 +0.5 1,488,286 + Dsui_1434 0.55 -1.5
Or see this region's nucleotide sequence