Experiment: ALP with Fusidic 0.02 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Dsui_1396 and Dsui_1397 overlap by 11 nucleotides Dsui_1397 and Dsui_1398 are separated by 157 nucleotides Dsui_1398 and Dsui_1399 overlap by 8 nucleotides
Dsui_1396: Dsui_1396 - type II secretory pathway, component ExeA (predicted ATPase), at 1,455,873 to 1,457,318
_1396
Dsui_1397: Dsui_1397 - response regulator with CheY-like receiver, AAA-type ATPase, and DNA-binding domains, at 1,457,308 to 1,458,771
_1397
Dsui_1398: Dsui_1398 - ABC-type transport system involved in resistance to organic solvents, ATPase component, at 1,458,929 to 1,459,750
_1398
Dsui_1399: Dsui_1399 - conserved hypothetical integral membrane protein, at 1,459,743 to 1,460,525
_1399
Position (kb)
1457
1458
1459 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1 at 1456.670 kb on + strand, within Dsui_1396 at 1456.759 kb on - strand, within Dsui_1396 at 1456.799 kb on - strand at 1456.921 kb on + strand at 1456.929 kb on - strand at 1457.001 kb on - strand, within Dsui_1396 at 1457.003 kb on + strand at 1457.011 kb on - strand, within Dsui_1396 at 1457.011 kb on - strand, within Dsui_1396 at 1457.086 kb on - strand, within Dsui_1396 at 1457.144 kb on + strand, within Dsui_1396 at 1457.156 kb on - strand, within Dsui_1396 at 1457.306 kb on + strand at 1457.341 kb on - strand at 1457.485 kb on - strand, within Dsui_1397 at 1457.501 kb on - strand at 1457.542 kb on - strand, within Dsui_1397 at 1457.582 kb on + strand at 1457.584 kb on - strand, within Dsui_1397 at 1457.673 kb on + strand, within Dsui_1397 at 1457.713 kb on + strand, within Dsui_1397 at 1457.721 kb on - strand, within Dsui_1397 at 1457.791 kb on + strand, within Dsui_1397 at 1457.867 kb on + strand at 1457.872 kb on + strand, within Dsui_1397 at 1457.944 kb on + strand, within Dsui_1397 at 1458.115 kb on - strand, within Dsui_1397 at 1458.126 kb on - strand, within Dsui_1397 at 1458.130 kb on - strand, within Dsui_1397 at 1458.286 kb on - strand, within Dsui_1397 at 1458.290 kb on + strand, within Dsui_1397 at 1458.307 kb on + strand at 1458.363 kb on + strand, within Dsui_1397 at 1458.371 kb on - strand, within Dsui_1397 at 1458.371 kb on - strand, within Dsui_1397 at 1458.539 kb on - strand, within Dsui_1397 at 1458.621 kb on + strand, within Dsui_1397 at 1458.644 kb on + strand at 1458.657 kb on - strand at 1458.677 kb on + strand at 1458.682 kb on + strand at 1458.685 kb on - strand at 1458.685 kb on - strand at 1458.697 kb on + strand at 1458.705 kb on - strand at 1458.705 kb on - strand at 1458.713 kb on + strand at 1458.727 kb on - strand at 1458.781 kb on + strand at 1458.810 kb on - strand at 1458.823 kb on + strand at 1458.872 kb on + strand at 1458.872 kb on + strand at 1458.878 kb on + strand at 1458.880 kb on - strand at 1458.880 kb on - strand at 1458.880 kb on - strand at 1458.880 kb on - strand at 1458.886 kb on - strand at 1459.033 kb on + strand, within Dsui_1398 at 1459.134 kb on - strand, within Dsui_1398 at 1459.148 kb on - strand at 1459.562 kb on - strand at 1459.638 kb on + strand, within Dsui_1398 at 1459.729 kb on + strand at 1459.730 kb on - strand at 1459.737 kb on - strand at 1459.737 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction ALP with Fusidic 0.02 mg/ml remove 1,456,670 + Dsui_1396 0.55 -0.5 1,456,759 - Dsui_1396 0.61 +0.5 1,456,799 - +0.5 1,456,921 + +0.5 1,456,929 - +0.5 1,457,001 - Dsui_1396 0.78 +0.5 1,457,003 + +0.5 1,457,011 - Dsui_1396 0.79 -3.6 1,457,011 - Dsui_1396 0.79 -2.3 1,457,086 - Dsui_1396 0.84 +0.5 1,457,144 + Dsui_1396 0.88 -1.5 1,457,156 - Dsui_1396 0.89 -2.1 1,457,306 + +0.5 1,457,341 - -0.5 1,457,485 - Dsui_1397 0.12 -1.5 1,457,501 - -0.5 1,457,542 - Dsui_1397 0.16 +0.5 1,457,582 + +0.5 1,457,584 - Dsui_1397 0.19 -0.5 1,457,673 + Dsui_1397 0.25 -0.5 1,457,713 + Dsui_1397 0.28 -0.5 1,457,721 - Dsui_1397 0.28 +0.5 1,457,791 + Dsui_1397 0.33 -1.1 1,457,867 + -0.5 1,457,872 + Dsui_1397 0.39 -1.5 1,457,944 + Dsui_1397 0.43 +0.5 1,458,115 - Dsui_1397 0.55 -2.7 1,458,126 - Dsui_1397 0.56 -3.4 1,458,130 - Dsui_1397 0.56 +0.5 1,458,286 - Dsui_1397 0.67 -1.1 1,458,290 + Dsui_1397 0.67 +0.5 1,458,307 + +0.5 1,458,363 + Dsui_1397 0.72 +0.5 1,458,371 - Dsui_1397 0.73 +0.5 1,458,371 - Dsui_1397 0.73 +0.5 1,458,539 - Dsui_1397 0.84 +0.5 1,458,621 + Dsui_1397 0.90 +0.5 1,458,644 + +0.5 1,458,657 - -0.5 1,458,677 + -0.5 1,458,682 + +0.5 1,458,685 - -0.5 1,458,685 - -0.5 1,458,697 + +0.5 1,458,705 - +0.5 1,458,705 - -1.5 1,458,713 + -1.1 1,458,727 - -0.5 1,458,781 + -0.5 1,458,810 - -2.7 1,458,823 + +0.5 1,458,872 + +0.5 1,458,872 + +0.5 1,458,878 + +0.5 1,458,880 - +0.5 1,458,880 - -1.1 1,458,880 - -0.5 1,458,880 - -1.1 1,458,886 - +0.5 1,459,033 + Dsui_1398 0.13 -4.5 1,459,134 - Dsui_1398 0.25 -1.1 1,459,148 - +0.5 1,459,562 - -0.5 1,459,638 + Dsui_1398 0.86 -3.8 1,459,729 + -4.3 1,459,730 - -1.5 1,459,737 - +0.5 1,459,737 - +0.5
Or see this region's nucleotide sequence