Experiment: ALP with Fusidic 0.02 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Dsui_1322 and Dsui_1323 are separated by 317 nucleotides Dsui_1323 and Dsui_1324 overlap by 13 nucleotides Dsui_1324 and Dsui_1325 are separated by 159 nucleotides Dsui_1325 and Dsui_1326 are separated by 54 nucleotides
Dsui_1322: Dsui_1322 - signal recognition particle protein, at 1,381,400 to 1,382,755
_1322
Dsui_1323: Dsui_1323 - soluble lytic murein transglycosylase-like protein, at 1,383,073 to 1,383,681
_1323
Dsui_1324: Dsui_1324 - prolyl-tRNA synthetase, family II, at 1,383,669 to 1,385,381
_1324
Dsui_1325: Dsui_1325 - ADP-ribose pyrophosphatase, at 1,385,541 to 1,386,065
_1325
Dsui_1326: Dsui_1326 - putative signal-transduction protein containing cAMP-binding and CBS domains, at 1,386,120 to 1,386,731
_1326
Position (kb)
1383
1384
1385
1386 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3
4 at 1382.707 kb on + strand at 1382.754 kb on + strand at 1382.774 kb on - strand at 1382.826 kb on + strand at 1382.831 kb on + strand at 1382.839 kb on - strand at 1382.851 kb on + strand at 1382.867 kb on + strand at 1382.867 kb on + strand at 1382.901 kb on - strand at 1382.971 kb on - strand at 1382.987 kb on + strand at 1382.988 kb on - strand at 1382.995 kb on - strand at 1383.002 kb on - strand at 1383.002 kb on - strand at 1383.026 kb on + strand at 1383.044 kb on + strand at 1383.049 kb on + strand at 1383.071 kb on + strand at 1383.071 kb on + strand at 1383.071 kb on + strand at 1383.079 kb on - strand at 1383.079 kb on - strand at 1383.122 kb on + strand at 1383.145 kb on - strand, within Dsui_1323 at 1383.150 kb on + strand, within Dsui_1323 at 1383.150 kb on + strand, within Dsui_1323 at 1383.158 kb on - strand, within Dsui_1323 at 1383.161 kb on + strand, within Dsui_1323 at 1383.187 kb on - strand, within Dsui_1323 at 1383.235 kb on - strand, within Dsui_1323 at 1383.264 kb on + strand, within Dsui_1323 at 1383.296 kb on - strand, within Dsui_1323 at 1383.318 kb on + strand, within Dsui_1323 at 1383.372 kb on - strand, within Dsui_1323 at 1383.411 kb on + strand, within Dsui_1323 at 1383.424 kb on - strand, within Dsui_1323 at 1383.439 kb on - strand, within Dsui_1323 at 1383.535 kb on + strand, within Dsui_1323 at 1383.571 kb on - strand, within Dsui_1323 at 1383.576 kb on - strand, within Dsui_1323 at 1383.576 kb on - strand, within Dsui_1323 at 1383.639 kb on + strand at 1383.647 kb on - strand at 1383.696 kb on - strand at 1385.442 kb on - strand at 1385.442 kb on - strand at 1385.541 kb on - strand at 1385.556 kb on + strand at 1385.645 kb on + strand, within Dsui_1325 at 1385.653 kb on - strand, within Dsui_1325 at 1385.656 kb on + strand, within Dsui_1325 at 1385.719 kb on - strand, within Dsui_1325 at 1385.894 kb on + strand, within Dsui_1325 at 1385.973 kb on - strand, within Dsui_1325 at 1386.009 kb on - strand, within Dsui_1325 at 1386.031 kb on + strand at 1386.031 kb on + strand at 1386.084 kb on + strand at 1386.084 kb on + strand at 1386.084 kb on + strand at 1386.092 kb on - strand at 1386.111 kb on - strand at 1386.112 kb on - strand at 1386.137 kb on + strand at 1386.137 kb on + strand at 1386.178 kb on + strand at 1386.215 kb on - strand, within Dsui_1326 at 1386.218 kb on + strand, within Dsui_1326 at 1386.218 kb on + strand, within Dsui_1326 at 1386.220 kb on - strand, within Dsui_1326 at 1386.220 kb on - strand, within Dsui_1326 at 1386.223 kb on + strand, within Dsui_1326 at 1386.239 kb on + strand, within Dsui_1326 at 1386.247 kb on - strand, within Dsui_1326 at 1386.274 kb on + strand, within Dsui_1326 at 1386.274 kb on + strand, within Dsui_1326 at 1386.274 kb on + strand, within Dsui_1326 at 1386.293 kb on + strand, within Dsui_1326 at 1386.305 kb on - strand, within Dsui_1326 at 1386.305 kb on - strand, within Dsui_1326 at 1386.311 kb on + strand, within Dsui_1326 at 1386.311 kb on + strand, within Dsui_1326 at 1386.311 kb on + strand, within Dsui_1326 at 1386.319 kb on - strand, within Dsui_1326 at 1386.319 kb on - strand, within Dsui_1326 at 1386.319 kb on - strand, within Dsui_1326 at 1386.330 kb on + strand, within Dsui_1326
Per-strain Table
Position Strand Gene LocusTag Fraction ALP with Fusidic 0.02 mg/ml remove 1,382,707 + +0.5 1,382,754 + -0.2 1,382,774 - -1.5 1,382,826 + -0.5 1,382,831 + -1.5 1,382,839 - -0.7 1,382,851 + -1.3 1,382,867 + -1.5 1,382,867 + +0.5 1,382,901 - -0.4 1,382,971 - +0.3 1,382,987 + +0.1 1,382,988 - -0.3 1,382,995 - -1.5 1,383,002 - -1.0 1,383,002 - -0.1 1,383,026 + -1.4 1,383,044 + -2.1 1,383,049 + +1.5 1,383,071 + -0.5 1,383,071 + -1.5 1,383,071 + -2.5 1,383,079 - -0.2 1,383,079 - -0.1 1,383,122 + -2.5 1,383,145 - Dsui_1323 0.12 +0.5 1,383,150 + Dsui_1323 0.13 -1.5 1,383,150 + Dsui_1323 0.13 +0.5 1,383,158 - Dsui_1323 0.14 -0.3 1,383,161 + Dsui_1323 0.14 -0.5 1,383,187 - Dsui_1323 0.19 +0.0 1,383,235 - Dsui_1323 0.27 -0.6 1,383,264 + Dsui_1323 0.31 -2.1 1,383,296 - Dsui_1323 0.37 +0.2 1,383,318 + Dsui_1323 0.40 -1.1 1,383,372 - Dsui_1323 0.49 -0.1 1,383,411 + Dsui_1323 0.56 +0.5 1,383,424 - Dsui_1323 0.58 -0.1 1,383,439 - Dsui_1323 0.60 +0.4 1,383,535 + Dsui_1323 0.76 -0.5 1,383,571 - Dsui_1323 0.82 +0.6 1,383,576 - Dsui_1323 0.83 -0.2 1,383,576 - Dsui_1323 0.83 -0.3 1,383,639 + -1.9 1,383,647 - +0.4 1,383,696 - -0.2 1,385,442 - +0.4 1,385,442 - +0.4 1,385,541 - +0.4 1,385,556 + +4.7 1,385,645 + Dsui_1325 0.20 +4.0 1,385,653 - Dsui_1325 0.21 +0.4 1,385,656 + Dsui_1325 0.22 +2.5 1,385,719 - Dsui_1325 0.34 -0.6 1,385,894 + Dsui_1325 0.67 +2.4 1,385,973 - Dsui_1325 0.82 -1.1 1,386,009 - Dsui_1325 0.89 -0.6 1,386,031 + +1.2 1,386,031 + +0.2 1,386,084 + +0.7 1,386,084 + +0.2 1,386,084 + +0.1 1,386,092 - -0.6 1,386,111 - +0.4 1,386,112 - -1.1 1,386,137 + +1.0 1,386,137 + +0.9 1,386,178 + -0.1 1,386,215 - Dsui_1326 0.16 -1.6 1,386,218 + Dsui_1326 0.16 +0.9 1,386,218 + Dsui_1326 0.16 +0.4 1,386,220 - Dsui_1326 0.16 -0.6 1,386,220 - Dsui_1326 0.16 +0.4 1,386,223 + Dsui_1326 0.17 +0.2 1,386,239 + Dsui_1326 0.19 +0.2 1,386,247 - Dsui_1326 0.21 +0.4 1,386,274 + Dsui_1326 0.25 +0.2 1,386,274 + Dsui_1326 0.25 +0.4 1,386,274 + Dsui_1326 0.25 +0.2 1,386,293 + Dsui_1326 0.28 +0.2 1,386,305 - Dsui_1326 0.30 -1.1 1,386,305 - Dsui_1326 0.30 -1.1 1,386,311 + Dsui_1326 0.31 +0.4 1,386,311 + Dsui_1326 0.31 +0.0 1,386,311 + Dsui_1326 0.31 +0.0 1,386,319 - Dsui_1326 0.33 -0.6 1,386,319 - Dsui_1326 0.33 +0.4 1,386,319 - Dsui_1326 0.33 -0.6 1,386,330 + Dsui_1326 0.34 -3.5
Or see this region's nucleotide sequence