Experiment: ALP with Fusidic 0.02 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Dsui_1309 and Dsui_1310 are separated by 97 nucleotides Dsui_1310 and Dsui_1311 are separated by 85 nucleotides Dsui_1311 and Dsui_1312 are separated by 168 nucleotides Dsui_1312 and Dsui_1313 overlap by 4 nucleotides
Dsui_1309: Dsui_1309 - acetylglutamate kinase, at 1,370,243 to 1,371,124
_1309
Dsui_1310: Dsui_1310 - molecular chaperone (small heat shock protein), at 1,371,222 to 1,371,653
_1310
Dsui_1311: Dsui_1311 - hypothetical protein, at 1,371,739 to 1,372,083
_1311
Dsui_1312: Dsui_1312 - NTP pyrophosphohydrolase, at 1,372,252 to 1,372,719
_1312
Dsui_1313: Dsui_1313 - Barstar, RNAse (barnase) inhibitor, at 1,372,716 to 1,373,162
_1313
Position (kb)
1371
1372
1373 Strain fitness (log2 ratio)
-2
-1
0
1 at 1371.192 kb on + strand at 1371.220 kb on + strand at 1371.222 kb on - strand at 1371.267 kb on - strand, within Dsui_1310 at 1371.403 kb on + strand, within Dsui_1310 at 1371.413 kb on + strand, within Dsui_1310 at 1371.452 kb on - strand, within Dsui_1310 at 1371.493 kb on + strand, within Dsui_1310 at 1371.619 kb on - strand at 1371.724 kb on - strand at 1371.729 kb on - strand at 1371.751 kb on + strand at 1371.781 kb on - strand, within Dsui_1311 at 1371.800 kb on + strand at 1371.812 kb on - strand, within Dsui_1311 at 1371.824 kb on - strand, within Dsui_1311 at 1371.829 kb on + strand at 1371.980 kb on - strand, within Dsui_1311 at 1372.076 kb on - strand at 1372.206 kb on - strand at 1372.314 kb on + strand at 1372.356 kb on + strand at 1372.356 kb on + strand at 1372.360 kb on - strand, within Dsui_1312 at 1372.402 kb on - strand, within Dsui_1312 at 1372.422 kb on + strand at 1372.489 kb on - strand, within Dsui_1312 at 1372.492 kb on + strand, within Dsui_1312 at 1372.647 kb on + strand, within Dsui_1312 at 1372.649 kb on + strand, within Dsui_1312 at 1372.649 kb on - strand, within Dsui_1312 at 1372.789 kb on - strand, within Dsui_1313 at 1372.824 kb on + strand at 1372.837 kb on - strand, within Dsui_1313 at 1372.837 kb on - strand, within Dsui_1313 at 1372.856 kb on - strand, within Dsui_1313 at 1372.879 kb on + strand at 1372.896 kb on + strand, within Dsui_1313 at 1372.904 kb on - strand, within Dsui_1313 at 1372.904 kb on - strand, within Dsui_1313 at 1372.904 kb on - strand, within Dsui_1313 at 1372.904 kb on - strand, within Dsui_1313 at 1372.909 kb on - strand, within Dsui_1313
Per-strain Table
Position Strand Gene LocusTag Fraction ALP with Fusidic 0.02 mg/ml remove 1,371,192 + +0.4 1,371,220 + +0.4 1,371,222 - -0.5 1,371,267 - Dsui_1310 0.10 -0.3 1,371,403 + Dsui_1310 0.42 +0.4 1,371,413 + Dsui_1310 0.44 -2.1 1,371,452 - Dsui_1310 0.53 -0.3 1,371,493 + Dsui_1310 0.63 +0.3 1,371,619 - -0.2 1,371,724 - -1.1 1,371,729 - +0.4 1,371,751 + -0.6 1,371,781 - Dsui_1311 0.12 -1.6 1,371,800 + -1.1 1,371,812 - Dsui_1311 0.21 +0.4 1,371,824 - Dsui_1311 0.25 -2.1 1,371,829 + +0.4 1,371,980 - Dsui_1311 0.70 -1.1 1,372,076 - +0.4 1,372,206 - -0.6 1,372,314 + +0.4 1,372,356 + +0.4 1,372,356 + +0.4 1,372,360 - Dsui_1312 0.23 -1.1 1,372,402 - Dsui_1312 0.32 -2.7 1,372,422 + +0.4 1,372,489 - Dsui_1312 0.51 -0.2 1,372,492 + Dsui_1312 0.51 -0.6 1,372,647 + Dsui_1312 0.84 -2.7 1,372,649 + Dsui_1312 0.85 -1.9 1,372,649 - Dsui_1312 0.85 -0.6 1,372,789 - Dsui_1313 0.16 -0.7 1,372,824 + -1.6 1,372,837 - Dsui_1313 0.27 -0.8 1,372,837 - Dsui_1313 0.27 +0.3 1,372,856 - Dsui_1313 0.31 -1.8 1,372,879 + +0.4 1,372,896 + Dsui_1313 0.40 -1.6 1,372,904 - Dsui_1313 0.42 -0.7 1,372,904 - Dsui_1313 0.42 -1.1 1,372,904 - Dsui_1313 0.42 -0.3 1,372,904 - Dsui_1313 0.42 -0.4 1,372,909 - Dsui_1313 0.43 -1.7
Or see this region's nucleotide sequence