Experiment: ALP with Fusidic 0.02 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Dsui_1300 and Dsui_1301 are separated by 46 nucleotides Dsui_1301 and Dsui_1302 overlap by 4 nucleotides Dsui_1302 and Dsui_1303 are separated by 3 nucleotides Dsui_1303 and Dsui_1304 are separated by 178 nucleotides
Dsui_1300: Dsui_1300 - sulfite oxidase-like oxidoreductase, at 1,363,222 to 1,364,160
_1300
Dsui_1301: Dsui_1301 - putative transmembrane transcriptional regulator (anti-sigma factor), at 1,364,207 to 1,364,983
_1301
Dsui_1302: Dsui_1302 - RNA polymerase sigma factor, sigma-70 family, at 1,364,980 to 1,365,480
_1302
Dsui_1303: Dsui_1303 - hypothetical protein, at 1,365,484 to 1,365,864
_1303
Dsui_1304: Dsui_1304 - cytochrome c553, at 1,366,043 to 1,366,684
_1304
Position (kb)
1364
1365
1366 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 1363.992 kb on - strand, within Dsui_1300 at 1363.999 kb on - strand, within Dsui_1300 at 1364.006 kb on - strand, within Dsui_1300 at 1364.040 kb on + strand at 1364.048 kb on - strand, within Dsui_1300 at 1364.048 kb on - strand, within Dsui_1300 at 1364.050 kb on + strand, within Dsui_1300 at 1364.058 kb on - strand, within Dsui_1300 at 1364.068 kb on + strand at 1364.075 kb on + strand at 1364.075 kb on + strand at 1364.085 kb on + strand at 1364.088 kb on - strand at 1364.093 kb on - strand at 1364.182 kb on - strand at 1364.187 kb on - strand at 1364.197 kb on - strand at 1364.233 kb on + strand at 1364.244 kb on - strand at 1364.254 kb on + strand at 1364.254 kb on + strand at 1364.262 kb on - strand at 1364.262 kb on - strand at 1364.267 kb on - strand at 1364.267 kb on - strand at 1364.267 kb on - strand at 1364.290 kb on - strand, within Dsui_1301 at 1364.381 kb on + strand, within Dsui_1301 at 1364.383 kb on + strand, within Dsui_1301 at 1364.391 kb on - strand, within Dsui_1301 at 1364.557 kb on + strand, within Dsui_1301 at 1364.575 kb on - strand, within Dsui_1301 at 1364.635 kb on - strand, within Dsui_1301 at 1364.640 kb on + strand, within Dsui_1301 at 1364.663 kb on + strand, within Dsui_1301 at 1364.725 kb on + strand, within Dsui_1301 at 1364.738 kb on - strand, within Dsui_1301 at 1364.840 kb on + strand, within Dsui_1301 at 1364.840 kb on + strand, within Dsui_1301 at 1364.840 kb on + strand at 1364.840 kb on + strand, within Dsui_1301 at 1364.845 kb on + strand, within Dsui_1301 at 1364.848 kb on - strand, within Dsui_1301 at 1364.903 kb on + strand, within Dsui_1301 at 1364.945 kb on + strand at 1364.945 kb on + strand at 1364.969 kb on + strand at 1364.992 kb on + strand at 1365.031 kb on + strand, within Dsui_1302 at 1365.099 kb on - strand, within Dsui_1302 at 1365.099 kb on - strand, within Dsui_1302 at 1365.195 kb on + strand, within Dsui_1302 at 1365.203 kb on - strand, within Dsui_1302 at 1365.203 kb on - strand, within Dsui_1302 at 1365.203 kb on - strand at 1365.372 kb on + strand, within Dsui_1302 at 1365.380 kb on - strand, within Dsui_1302 at 1365.380 kb on - strand, within Dsui_1302 at 1365.423 kb on + strand, within Dsui_1302 at 1365.569 kb on + strand, within Dsui_1303 at 1365.570 kb on - strand, within Dsui_1303 at 1365.630 kb on - strand, within Dsui_1303 at 1365.740 kb on - strand, within Dsui_1303 at 1365.771 kb on + strand, within Dsui_1303 at 1365.781 kb on + strand, within Dsui_1303 at 1365.842 kb on - strand at 1365.868 kb on + strand at 1365.873 kb on + strand at 1365.878 kb on + strand at 1365.878 kb on + strand at 1365.886 kb on - strand at 1365.949 kb on + strand at 1365.952 kb on + strand at 1365.954 kb on + strand at 1365.957 kb on - strand at 1366.049 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction ALP with Fusidic 0.02 mg/ml remove 1,363,992 - Dsui_1300 0.82 -0.4 1,363,999 - Dsui_1300 0.83 +0.1 1,364,006 - Dsui_1300 0.83 +0.5 1,364,040 + -1.1 1,364,048 - Dsui_1300 0.88 -0.1 1,364,048 - Dsui_1300 0.88 -1.1 1,364,050 + Dsui_1300 0.88 +0.5 1,364,058 - Dsui_1300 0.89 +1.5 1,364,068 + +0.5 1,364,075 + +0.5 1,364,075 + -1.9 1,364,085 + +0.5 1,364,088 - -0.2 1,364,093 - -0.1 1,364,182 - -0.9 1,364,187 - +0.3 1,364,197 - -0.4 1,364,233 + -0.5 1,364,244 - -0.2 1,364,254 + -0.5 1,364,254 + -0.5 1,364,262 - +0.1 1,364,262 - -0.6 1,364,267 - -0.6 1,364,267 - -0.5 1,364,267 - -0.7 1,364,290 - Dsui_1301 0.11 -0.6 1,364,381 + Dsui_1301 0.22 +0.5 1,364,383 + Dsui_1301 0.23 -1.5 1,364,391 - Dsui_1301 0.24 +0.3 1,364,557 + Dsui_1301 0.45 -0.5 1,364,575 - Dsui_1301 0.47 -0.5 1,364,635 - Dsui_1301 0.55 -0.5 1,364,640 + Dsui_1301 0.56 -0.5 1,364,663 + Dsui_1301 0.59 -2.1 1,364,725 + Dsui_1301 0.67 -1.1 1,364,738 - Dsui_1301 0.68 -1.1 1,364,840 + Dsui_1301 0.81 -0.5 1,364,840 + Dsui_1301 0.81 -0.5 1,364,840 + +0.5 1,364,840 + Dsui_1301 0.81 +0.5 1,364,845 + Dsui_1301 0.82 -2.3 1,364,848 - Dsui_1301 0.82 -1.4 1,364,903 + Dsui_1301 0.90 +0.5 1,364,945 + -1.9 1,364,945 + -0.5 1,364,969 + +0.5 1,364,992 + +0.5 1,365,031 + Dsui_1302 0.10 +0.5 1,365,099 - Dsui_1302 0.24 -2.2 1,365,099 - Dsui_1302 0.24 -2.5 1,365,195 + Dsui_1302 0.43 -1.9 1,365,203 - Dsui_1302 0.45 +0.5 1,365,203 - Dsui_1302 0.45 -0.5 1,365,203 - +0.5 1,365,372 + Dsui_1302 0.78 -1.5 1,365,380 - Dsui_1302 0.80 +0.5 1,365,380 - Dsui_1302 0.80 +0.0 1,365,423 + Dsui_1302 0.88 +0.5 1,365,569 + Dsui_1303 0.22 -1.9 1,365,570 - Dsui_1303 0.23 -1.1 1,365,630 - Dsui_1303 0.38 +0.0 1,365,740 - Dsui_1303 0.67 -0.4 1,365,771 + Dsui_1303 0.75 -1.5 1,365,781 + Dsui_1303 0.78 -0.5 1,365,842 - +0.1 1,365,868 + -3.0 1,365,873 + -0.5 1,365,878 + -1.5 1,365,878 + +0.5 1,365,886 - -0.7 1,365,949 + +0.5 1,365,952 + -3.1 1,365,954 + -2.4 1,365,957 - +0.4 1,366,049 - -0.0
Or see this region's nucleotide sequence