Experiment: ALP with Fusidic 0.02 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Dsui_1182 and Dsui_1183 overlap by 4 nucleotides Dsui_1183 and Dsui_1184 are separated by 120 nucleotides Dsui_1184 and Dsui_1185 are separated by 39 nucleotides Dsui_1185 and Dsui_1186 are separated by 12 nucleotides
Dsui_1182: Dsui_1182 - diaminopimelate decarboxylase, at 1,235,606 to 1,236,862
_1182
Dsui_1183: Dsui_1183 - hypothetical protein, at 1,236,859 to 1,237,032
_1183
Dsui_1184: Dsui_1184 - iron donor protein CyaY, at 1,237,153 to 1,237,470
_1184
Dsui_1185: Dsui_1185 - hypothetical protein involved in intracellular sulfur reduction, at 1,237,510 to 1,237,866
_1185
Dsui_1186: Dsui_1186 - penicillin-binding protein, 1A family, at 1,237,879 to 1,240,194
_1186
Position (kb)
1237
1238 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 1237.078 kb on - strand at 1237.104 kb on + strand at 1237.112 kb on - strand at 1237.116 kb on + strand at 1237.123 kb on - strand at 1237.124 kb on - strand at 1237.124 kb on - strand at 1237.124 kb on - strand at 1237.462 kb on - strand at 1237.493 kb on - strand at 1237.517 kb on + strand at 1237.525 kb on - strand at 1237.541 kb on - strand at 1237.552 kb on - strand, within Dsui_1185 at 1237.615 kb on - strand, within Dsui_1185 at 1237.615 kb on - strand, within Dsui_1185 at 1237.615 kb on - strand, within Dsui_1185 at 1237.852 kb on + strand at 1237.962 kb on - strand at 1237.970 kb on + strand at 1237.970 kb on + strand at 1237.992 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction ALP with Fusidic 0.02 mg/ml remove 1,237,078 - -0.4 1,237,104 + +0.3 1,237,112 - -3.7 1,237,116 + -0.2 1,237,123 - -1.1 1,237,124 - -0.5 1,237,124 - -0.4 1,237,124 - -1.1 1,237,462 - +1.2 1,237,493 - -0.9 1,237,517 + +1.7 1,237,525 - +1.1 1,237,541 - +0.0 1,237,552 - Dsui_1185 0.12 -0.4 1,237,615 - Dsui_1185 0.29 -0.4 1,237,615 - Dsui_1185 0.29 -0.4 1,237,615 - Dsui_1185 0.29 +0.2 1,237,852 + -1.8 1,237,962 - -0.0 1,237,970 + -1.5 1,237,970 + +0.0 1,237,992 + +0.5
Or see this region's nucleotide sequence