Experiment: ALP with Fusidic 0.02 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Dsui_1163 and Dsui_1164 are separated by 49 nucleotides Dsui_1164 and Dsui_1165 overlap by 8 nucleotides Dsui_1165 and Dsui_1166 are separated by 369 nucleotides
Dsui_1163: Dsui_1163 - hydrogenase accessory protein HypB, at 1,219,522 to 1,220,718
_1163
Dsui_1164: Dsui_1164 - hydrogenase nickel insertion protein HypA, at 1,220,768 to 1,221,112
_1164
Dsui_1165: Dsui_1165 - Ni,Fe-hydrogenase I large subunit, at 1,221,105 to 1,222,094
_1165
Dsui_1166: Dsui_1166 - cytochrome c peroxidase, at 1,222,464 to 1,223,474
_1166
Position (kb)
1221
1222
1223 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 1220.218 kb on + strand, within Dsui_1163 at 1220.236 kb on - strand, within Dsui_1163 at 1220.402 kb on - strand, within Dsui_1163 at 1220.409 kb on - strand, within Dsui_1163 at 1220.414 kb on - strand, within Dsui_1163 at 1220.425 kb on + strand, within Dsui_1163 at 1220.456 kb on - strand, within Dsui_1163 at 1220.470 kb on + strand, within Dsui_1163 at 1220.565 kb on + strand, within Dsui_1163 at 1220.585 kb on - strand, within Dsui_1163 at 1220.610 kb on - strand at 1220.612 kb on + strand at 1220.615 kb on - strand at 1220.683 kb on + strand at 1220.711 kb on + strand at 1220.719 kb on - strand at 1220.724 kb on - strand at 1220.849 kb on + strand at 1220.885 kb on + strand, within Dsui_1164 at 1220.896 kb on + strand, within Dsui_1164 at 1220.927 kb on - strand, within Dsui_1164 at 1220.949 kb on + strand, within Dsui_1164 at 1220.954 kb on + strand at 1220.990 kb on - strand, within Dsui_1164 at 1221.005 kb on - strand, within Dsui_1164 at 1221.035 kb on - strand, within Dsui_1164 at 1221.044 kb on + strand at 1221.052 kb on - strand, within Dsui_1164 at 1221.054 kb on - strand, within Dsui_1164 at 1221.095 kb on - strand at 1221.095 kb on - strand at 1221.138 kb on - strand at 1221.159 kb on + strand at 1221.293 kb on - strand, within Dsui_1165 at 1221.476 kb on + strand at 1221.494 kb on - strand at 1221.603 kb on - strand at 1221.727 kb on + strand at 1221.728 kb on - strand, within Dsui_1165 at 1221.786 kb on + strand, within Dsui_1165 at 1221.904 kb on + strand, within Dsui_1165 at 1221.904 kb on + strand at 1221.917 kb on - strand, within Dsui_1165 at 1222.078 kb on + strand at 1222.078 kb on + strand at 1222.093 kb on + strand at 1222.124 kb on + strand at 1222.125 kb on + strand at 1222.342 kb on + strand at 1222.350 kb on - strand at 1222.447 kb on + strand at 1222.509 kb on + strand at 1222.529 kb on + strand at 1222.580 kb on + strand, within Dsui_1166 at 1222.676 kb on + strand at 1222.676 kb on + strand, within Dsui_1166 at 1222.684 kb on - strand, within Dsui_1166 at 1222.739 kb on - strand, within Dsui_1166 at 1222.765 kb on + strand, within Dsui_1166 at 1222.773 kb on - strand at 1222.781 kb on + strand, within Dsui_1166 at 1222.781 kb on + strand, within Dsui_1166 at 1222.789 kb on - strand at 1222.857 kb on + strand, within Dsui_1166 at 1222.859 kb on - strand at 1222.864 kb on + strand, within Dsui_1166 at 1222.876 kb on - strand at 1222.927 kb on + strand, within Dsui_1166 at 1223.062 kb on + strand, within Dsui_1166 at 1223.070 kb on - strand at 1223.070 kb on - strand, within Dsui_1166
Per-strain Table
Position Strand Gene LocusTag Fraction ALP with Fusidic 0.02 mg/ml remove 1,220,218 + Dsui_1163 0.58 -0.6 1,220,236 - Dsui_1163 0.60 +0.4 1,220,402 - Dsui_1163 0.74 +0.6 1,220,409 - Dsui_1163 0.74 +0.2 1,220,414 - Dsui_1163 0.75 -0.8 1,220,425 + Dsui_1163 0.75 -0.6 1,220,456 - Dsui_1163 0.78 -0.1 1,220,470 + Dsui_1163 0.79 -0.6 1,220,565 + Dsui_1163 0.87 +0.4 1,220,585 - Dsui_1163 0.89 -0.1 1,220,610 - +0.1 1,220,612 + -0.6 1,220,615 - +0.3 1,220,683 + -0.6 1,220,711 + -0.6 1,220,719 - -0.6 1,220,724 - -0.3 1,220,849 + +0.4 1,220,885 + Dsui_1164 0.34 -0.6 1,220,896 + Dsui_1164 0.37 -2.1 1,220,927 - Dsui_1164 0.46 +1.2 1,220,949 + Dsui_1164 0.52 -0.6 1,220,954 + +0.4 1,220,990 - Dsui_1164 0.64 +0.1 1,221,005 - Dsui_1164 0.69 +0.4 1,221,035 - Dsui_1164 0.77 +0.2 1,221,044 + +0.4 1,221,052 - Dsui_1164 0.82 -0.9 1,221,054 - Dsui_1164 0.83 -0.8 1,221,095 - -0.2 1,221,095 - +1.1 1,221,138 - +0.1 1,221,159 + +0.4 1,221,293 - Dsui_1165 0.19 -0.6 1,221,476 + +0.4 1,221,494 - +0.4 1,221,603 - -0.6 1,221,727 + +0.4 1,221,728 - Dsui_1165 0.63 -2.4 1,221,786 + Dsui_1165 0.69 -1.1 1,221,904 + Dsui_1165 0.81 -1.6 1,221,904 + +0.4 1,221,917 - Dsui_1165 0.82 -1.1 1,222,078 + -1.1 1,222,078 + -1.1 1,222,093 + -1.1 1,222,124 + -0.6 1,222,125 + +0.4 1,222,342 + +0.4 1,222,350 - +0.4 1,222,447 + -1.9 1,222,509 + -0.6 1,222,529 + +0.4 1,222,580 + Dsui_1166 0.11 -2.1 1,222,676 + +0.4 1,222,676 + Dsui_1166 0.21 +0.4 1,222,684 - Dsui_1166 0.22 -1.9 1,222,739 - Dsui_1166 0.27 -2.9 1,222,765 + Dsui_1166 0.30 +0.4 1,222,773 - -1.6 1,222,781 + Dsui_1166 0.31 +0.4 1,222,781 + Dsui_1166 0.31 -0.6 1,222,789 - +0.4 1,222,857 + Dsui_1166 0.39 -1.9 1,222,859 - -1.1 1,222,864 + Dsui_1166 0.40 +0.4 1,222,876 - +0.4 1,222,927 + Dsui_1166 0.46 +0.4 1,223,062 + Dsui_1166 0.59 -1.1 1,223,070 - +0.4 1,223,070 - Dsui_1166 0.60 -0.6
Or see this region's nucleotide sequence