Experiment: ALP with Fusidic 0.02 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Dsui_1099 and Dsui_1100 are separated by 9 nucleotides Dsui_1100 and Dsui_1101 are separated by 3 nucleotides Dsui_1101 and Dsui_1102 are separated by 81 nucleotides
Dsui_1099: Dsui_1099 - UDP-N-acetylglucosamine diphosphorylase/glucosamine-1-phosphate N-acetyltransferase, at 1,142,307 to 1,143,665
_1099
Dsui_1100: Dsui_1100 - hypothetical protein, at 1,143,675 to 1,143,983
_1100
Dsui_1101: Dsui_1101 - glucosamine--fructose-6-phosphate aminotransferase, isomerizing, at 1,143,987 to 1,145,813
_1101
Dsui_1102: Dsui_1102 - hypothetical protein, at 1,145,895 to 1,146,902
_1102
Position (kb)
1143
1144
1145
1146 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 1145.934 kb on - strand at 1145.996 kb on - strand, within Dsui_1102 at 1146.123 kb on - strand, within Dsui_1102 at 1146.170 kb on + strand, within Dsui_1102 at 1146.175 kb on + strand, within Dsui_1102 at 1146.175 kb on + strand, within Dsui_1102 at 1146.183 kb on - strand, within Dsui_1102 at 1146.185 kb on + strand, within Dsui_1102 at 1146.206 kb on + strand, within Dsui_1102 at 1146.214 kb on - strand, within Dsui_1102 at 1146.214 kb on - strand, within Dsui_1102 at 1146.214 kb on - strand, within Dsui_1102 at 1146.214 kb on - strand, within Dsui_1102 at 1146.329 kb on + strand, within Dsui_1102 at 1146.382 kb on - strand, within Dsui_1102 at 1146.681 kb on + strand, within Dsui_1102 at 1146.683 kb on + strand, within Dsui_1102 at 1146.691 kb on - strand, within Dsui_1102 at 1146.725 kb on - strand, within Dsui_1102 at 1146.728 kb on + strand, within Dsui_1102 at 1146.736 kb on - strand, within Dsui_1102 at 1146.736 kb on - strand, within Dsui_1102 at 1146.753 kb on - strand, within Dsui_1102
Per-strain Table
Position Strand Gene LocusTag Fraction ALP with Fusidic 0.02 mg/ml remove 1,145,934 - +0.0 1,145,996 - Dsui_1102 0.10 +0.1 1,146,123 - Dsui_1102 0.23 -2.8 1,146,170 + Dsui_1102 0.27 +0.5 1,146,175 + Dsui_1102 0.28 +0.2 1,146,175 + Dsui_1102 0.28 -0.5 1,146,183 - Dsui_1102 0.29 +0.5 1,146,185 + Dsui_1102 0.29 -0.1 1,146,206 + Dsui_1102 0.31 +0.0 1,146,214 - Dsui_1102 0.32 -2.6 1,146,214 - Dsui_1102 0.32 -1.1 1,146,214 - Dsui_1102 0.32 -1.1 1,146,214 - Dsui_1102 0.32 -0.5 1,146,329 + Dsui_1102 0.43 +0.3 1,146,382 - Dsui_1102 0.48 -1.5 1,146,681 + Dsui_1102 0.78 -0.0 1,146,683 + Dsui_1102 0.78 -0.2 1,146,691 - Dsui_1102 0.79 -0.5 1,146,725 - Dsui_1102 0.82 +0.5 1,146,728 + Dsui_1102 0.83 +0.1 1,146,736 - Dsui_1102 0.83 -2.9 1,146,736 - Dsui_1102 0.83 -1.5 1,146,753 - Dsui_1102 0.85 +0.5
Or see this region's nucleotide sequence