Experiment: ALP with Fusidic 0.02 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Dsui_0983 and Dsui_0984 are separated by 18 nucleotides Dsui_0984 and Dsui_0985 are separated by 62 nucleotides Dsui_0985 and Dsui_0986 overlap by 4 nucleotides Dsui_0986 and Dsui_0987 overlap by 1 nucleotides
Dsui_0983: Dsui_0983 - PAS domain S-box/diguanylate cyclase (GGDEF) domain-containing protein, at 1,010,975 to 1,013,275
_0983
Dsui_0984: Dsui_0984 - isopropylmalate/homocitrate/citramalate synthase, at 1,013,294 to 1,014,202
_0984
Dsui_0985: Dsui_0985 - putative Fe-S protein, at 1,014,265 to 1,014,480
_0985
Dsui_0986: Dsui_0986 - putative Fe-S protein, at 1,014,477 to 1,014,671
_0986
Dsui_0987: Dsui_0987 - Zn-dependent hydrolase, glyoxylase, at 1,014,671 to 1,015,606
_0987
Position (kb)
1013
1014
1015 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3 at 1012.567 kb on - strand, within Dsui_0983 at 1012.789 kb on - strand at 1012.810 kb on - strand, within Dsui_0983 at 1012.930 kb on - strand, within Dsui_0983 at 1012.963 kb on + strand, within Dsui_0983 at 1012.971 kb on - strand, within Dsui_0983 at 1013.081 kb on + strand at 1013.089 kb on - strand at 1013.126 kb on - strand at 1013.144 kb on + strand at 1013.149 kb on + strand at 1013.149 kb on + strand at 1013.249 kb on + strand at 1013.311 kb on + strand at 1013.331 kb on + strand at 1013.382 kb on + strand at 1013.531 kb on - strand, within Dsui_0984 at 1013.531 kb on - strand, within Dsui_0984 at 1013.606 kb on + strand, within Dsui_0984 at 1013.640 kb on - strand, within Dsui_0984 at 1013.713 kb on - strand, within Dsui_0984 at 1013.713 kb on - strand, within Dsui_0984 at 1013.825 kb on - strand, within Dsui_0984 at 1013.829 kb on - strand, within Dsui_0984 at 1013.837 kb on - strand at 1013.925 kb on + strand, within Dsui_0984 at 1013.944 kb on - strand, within Dsui_0984 at 1013.966 kb on - strand at 1014.067 kb on + strand, within Dsui_0984 at 1014.108 kb on + strand, within Dsui_0984 at 1014.262 kb on + strand at 1014.262 kb on + strand at 1014.274 kb on + strand at 1014.274 kb on + strand at 1014.311 kb on + strand, within Dsui_0985 at 1014.311 kb on + strand, within Dsui_0985 at 1014.668 kb on - strand at 1014.717 kb on - strand at 1014.801 kb on - strand at 1014.818 kb on + strand, within Dsui_0987 at 1014.818 kb on + strand, within Dsui_0987 at 1014.826 kb on - strand, within Dsui_0987 at 1014.845 kb on + strand, within Dsui_0987 at 1014.984 kb on + strand, within Dsui_0987 at 1015.049 kb on + strand, within Dsui_0987 at 1015.113 kb on + strand, within Dsui_0987
Per-strain Table
Position Strand Gene LocusTag Fraction ALP with Fusidic 0.02 mg/ml remove 1,012,567 - Dsui_0983 0.69 -1.0 1,012,789 - +0.6 1,012,810 - Dsui_0983 0.80 -1.0 1,012,930 - Dsui_0983 0.85 -0.4 1,012,963 + Dsui_0983 0.86 +0.6 1,012,971 - Dsui_0983 0.87 +1.6 1,013,081 + -0.3 1,013,089 - -0.4 1,013,126 - +0.6 1,013,144 + -0.2 1,013,149 + +2.3 1,013,149 + -2.6 1,013,249 + +0.4 1,013,311 + +0.8 1,013,331 + +1.8 1,013,382 + +3.4 1,013,531 - Dsui_0984 0.26 -0.4 1,013,531 - Dsui_0984 0.26 +0.6 1,013,606 + Dsui_0984 0.34 +1.4 1,013,640 - Dsui_0984 0.38 -0.4 1,013,713 - Dsui_0984 0.46 +0.6 1,013,713 - Dsui_0984 0.46 -4.0 1,013,825 - Dsui_0984 0.58 -1.4 1,013,829 - Dsui_0984 0.59 +0.6 1,013,837 - +0.6 1,013,925 + Dsui_0984 0.69 -0.4 1,013,944 - Dsui_0984 0.72 -1.4 1,013,966 - +0.6 1,014,067 + Dsui_0984 0.85 +1.2 1,014,108 + Dsui_0984 0.90 +0.5 1,014,262 + +0.7 1,014,262 + -0.4 1,014,274 + -0.2 1,014,274 + -0.1 1,014,311 + Dsui_0985 0.21 -0.2 1,014,311 + Dsui_0985 0.21 -0.3 1,014,668 - +0.6 1,014,717 - -3.2 1,014,801 - +0.6 1,014,818 + Dsui_0987 0.16 -0.2 1,014,818 + Dsui_0987 0.16 -0.1 1,014,826 - Dsui_0987 0.17 -0.4 1,014,845 + Dsui_0987 0.19 +0.2 1,014,984 + Dsui_0987 0.33 -0.0 1,015,049 + Dsui_0987 0.40 -0.1 1,015,113 + Dsui_0987 0.47 +0.1
Or see this region's nucleotide sequence