Experiment: ALP with Fusidic 0.02 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Dsui_0982 and Dsui_0983 are separated by 188 nucleotides Dsui_0983 and Dsui_0984 are separated by 18 nucleotides Dsui_0984 and Dsui_0985 are separated by 62 nucleotides
Dsui_0982: Dsui_0982 - acetyl/propionyl-CoA carboxylase, alpha subunit, at 1,008,783 to 1,010,786
_0982
Dsui_0983: Dsui_0983 - PAS domain S-box/diguanylate cyclase (GGDEF) domain-containing protein, at 1,010,975 to 1,013,275
_0983
Dsui_0984: Dsui_0984 - isopropylmalate/homocitrate/citramalate synthase, at 1,013,294 to 1,014,202
_0984
Dsui_0985: Dsui_0985 - putative Fe-S protein, at 1,014,265 to 1,014,480
_0985
Position (kb)
1010
1011
1012
1013
1014 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3 at 1009.978 kb on - strand, within Dsui_0982 at 1010.003 kb on - strand, within Dsui_0982 at 1010.083 kb on + strand, within Dsui_0982 at 1010.390 kb on + strand, within Dsui_0982 at 1010.537 kb on - strand, within Dsui_0982 at 1010.615 kb on + strand at 1010.686 kb on + strand at 1010.754 kb on - strand at 1010.780 kb on + strand at 1010.863 kb on + strand at 1010.876 kb on - strand at 1011.031 kb on + strand at 1011.114 kb on + strand at 1011.132 kb on + strand at 1011.132 kb on + strand at 1011.369 kb on - strand, within Dsui_0983 at 1011.385 kb on + strand, within Dsui_0983 at 1011.488 kb on - strand, within Dsui_0983 at 1011.507 kb on + strand, within Dsui_0983 at 1011.517 kb on + strand, within Dsui_0983 at 1011.635 kb on - strand, within Dsui_0983 at 1011.642 kb on + strand, within Dsui_0983 at 1011.706 kb on + strand, within Dsui_0983 at 1011.767 kb on - strand, within Dsui_0983 at 1011.957 kb on + strand at 1011.965 kb on - strand, within Dsui_0983 at 1011.982 kb on - strand, within Dsui_0983 at 1012.022 kb on + strand, within Dsui_0983 at 1012.036 kb on + strand, within Dsui_0983 at 1012.048 kb on + strand, within Dsui_0983 at 1012.048 kb on + strand, within Dsui_0983 at 1012.567 kb on - strand, within Dsui_0983 at 1012.789 kb on - strand at 1012.810 kb on - strand, within Dsui_0983 at 1012.930 kb on - strand, within Dsui_0983 at 1012.963 kb on + strand, within Dsui_0983 at 1012.971 kb on - strand, within Dsui_0983 at 1013.081 kb on + strand at 1013.089 kb on - strand at 1013.126 kb on - strand at 1013.144 kb on + strand at 1013.149 kb on + strand at 1013.149 kb on + strand at 1013.249 kb on + strand at 1013.311 kb on + strand at 1013.331 kb on + strand at 1013.382 kb on + strand at 1013.531 kb on - strand, within Dsui_0984 at 1013.531 kb on - strand, within Dsui_0984 at 1013.606 kb on + strand, within Dsui_0984 at 1013.640 kb on - strand, within Dsui_0984 at 1013.713 kb on - strand, within Dsui_0984 at 1013.713 kb on - strand, within Dsui_0984 at 1013.825 kb on - strand, within Dsui_0984 at 1013.829 kb on - strand, within Dsui_0984 at 1013.837 kb on - strand at 1013.925 kb on + strand, within Dsui_0984 at 1013.944 kb on - strand, within Dsui_0984 at 1013.966 kb on - strand at 1014.067 kb on + strand, within Dsui_0984 at 1014.108 kb on + strand, within Dsui_0984 at 1014.262 kb on + strand at 1014.262 kb on + strand at 1014.274 kb on + strand at 1014.274 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction ALP with Fusidic 0.02 mg/ml remove 1,009,978 - Dsui_0982 0.60 -0.4 1,010,003 - Dsui_0982 0.61 -1.4 1,010,083 + Dsui_0982 0.65 -3.4 1,010,390 + Dsui_0982 0.80 +0.5 1,010,537 - Dsui_0982 0.88 -1.7 1,010,615 + -1.7 1,010,686 + +0.6 1,010,754 - +0.6 1,010,780 + -1.0 1,010,863 + +0.6 1,010,876 - -1.0 1,011,031 + +0.2 1,011,114 + -1.2 1,011,132 + +0.8 1,011,132 + +1.0 1,011,369 - Dsui_0983 0.17 -1.0 1,011,385 + Dsui_0983 0.18 +0.7 1,011,488 - Dsui_0983 0.22 -0.4 1,011,507 + Dsui_0983 0.23 +0.3 1,011,517 + Dsui_0983 0.24 -0.5 1,011,635 - Dsui_0983 0.29 +0.6 1,011,642 + Dsui_0983 0.29 +0.4 1,011,706 + Dsui_0983 0.32 -1.0 1,011,767 - Dsui_0983 0.34 -1.0 1,011,957 + +1.6 1,011,965 - Dsui_0983 0.43 -1.0 1,011,982 - Dsui_0983 0.44 -2.7 1,012,022 + Dsui_0983 0.46 +1.1 1,012,036 + Dsui_0983 0.46 -0.0 1,012,048 + Dsui_0983 0.47 -0.6 1,012,048 + Dsui_0983 0.47 -0.2 1,012,567 - Dsui_0983 0.69 -1.0 1,012,789 - +0.6 1,012,810 - Dsui_0983 0.80 -1.0 1,012,930 - Dsui_0983 0.85 -0.4 1,012,963 + Dsui_0983 0.86 +0.6 1,012,971 - Dsui_0983 0.87 +1.6 1,013,081 + -0.3 1,013,089 - -0.4 1,013,126 - +0.6 1,013,144 + -0.2 1,013,149 + +2.3 1,013,149 + -2.6 1,013,249 + +0.4 1,013,311 + +0.8 1,013,331 + +1.8 1,013,382 + +3.4 1,013,531 - Dsui_0984 0.26 -0.4 1,013,531 - Dsui_0984 0.26 +0.6 1,013,606 + Dsui_0984 0.34 +1.4 1,013,640 - Dsui_0984 0.38 -0.4 1,013,713 - Dsui_0984 0.46 +0.6 1,013,713 - Dsui_0984 0.46 -4.0 1,013,825 - Dsui_0984 0.58 -1.4 1,013,829 - Dsui_0984 0.59 +0.6 1,013,837 - +0.6 1,013,925 + Dsui_0984 0.69 -0.4 1,013,944 - Dsui_0984 0.72 -1.4 1,013,966 - +0.6 1,014,067 + Dsui_0984 0.85 +1.2 1,014,108 + Dsui_0984 0.90 +0.5 1,014,262 + +0.7 1,014,262 + -0.4 1,014,274 + -0.2 1,014,274 + -0.1
Or see this region's nucleotide sequence