Experiment: ALP with Fusidic 0.02 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Dsui_0947 and Dsui_0948 are separated by 5 nucleotides Dsui_0948 and Dsui_0949 are separated by 40 nucleotides Dsui_0949 and Dsui_0950 overlap by 4 nucleotides Dsui_0950 and Dsui_0951 are separated by 169 nucleotides
Dsui_0947: Dsui_0947 - hypothetical protein, at 974,567 to 975,425
_0947
Dsui_0948: Dsui_0948 - hypothetical protein, at 975,431 to 975,850
_0948
Dsui_0949: Dsui_0949 - hypothetical protein, at 975,891 to 976,106
_0949
Dsui_0950: Dsui_0950 - acyl dehydratase, at 976,103 to 976,603
_0950
Dsui_0951: Dsui_0951 - nucleoside-diphosphate-sugar epimerase, at 976,773 to 977,732
_0951
Position (kb) 975
976
977 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 974.959 kb on + strand, within Dsui_0947 at 975.083 kb on - strand, within Dsui_0947 at 975.355 kb on - strand at 975.410 kb on - strand at 975.468 kb on + strand at 975.543 kb on + strand at 975.590 kb on + strand, within Dsui_0948 at 975.599 kb on - strand at 975.918 kb on - strand, within Dsui_0949 at 976.147 kb on + strand at 976.398 kb on + strand, within Dsui_0950 at 976.530 kb on - strand, within Dsui_0950 at 976.642 kb on - strand at 976.700 kb on + strand at 976.700 kb on + strand at 976.893 kb on + strand, within Dsui_0951 at 976.893 kb on + strand, within Dsui_0951 at 977.020 kb on - strand, within Dsui_0951 at 977.048 kb on + strand, within Dsui_0951
Per-strain Table
Position Strand Gene LocusTag Fraction ALP with Fusidic 0.02 mg/ml remove 974,959 + Dsui_0947 0.46 +0.5 975,083 - Dsui_0947 0.60 -1.1 975,355 - +0.5 975,410 - -1.5 975,468 + -1.1 975,543 + +0.5 975,590 + Dsui_0948 0.38 -0.5 975,599 - +0.5 975,918 - Dsui_0949 0.12 +0.6 976,147 + +1.5 976,398 + Dsui_0950 0.59 -3.6 976,530 - Dsui_0950 0.85 -0.3 976,642 - +0.5 976,700 + -0.5 976,700 + +0.5 976,893 + Dsui_0951 0.12 -0.6 976,893 + Dsui_0951 0.12 +1.4 977,020 - Dsui_0951 0.26 -1.5 977,048 + Dsui_0951 0.29 -0.2
Or see this region's nucleotide sequence