Experiment: ALP with Fusidic 0.02 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Dsui_0921 and Dsui_0922 are separated by 97 nucleotides Dsui_0922 and Dsui_0923 are separated by 29 nucleotides
Dsui_0921: Dsui_0921 - PAS domain S-box, at 944,617 to 948,909
_0921
Dsui_0922: Dsui_0922 - outer membrane receptor protein, at 949,007 to 951,028
_0922
Dsui_0923: Dsui_0923 - putative iron-regulated membrane protein, at 951,058 to 952,206
_0923
Position (kb) 949
950
951
952 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 948.018 kb on + strand at 948.146 kb on - strand, within Dsui_0921 at 948.150 kb on + strand, within Dsui_0921 at 948.203 kb on - strand, within Dsui_0921 at 948.299 kb on + strand, within Dsui_0921 at 948.317 kb on - strand, within Dsui_0921 at 948.451 kb on + strand, within Dsui_0921 at 948.616 kb on + strand at 948.616 kb on + strand at 948.624 kb on - strand at 948.678 kb on + strand at 948.693 kb on - strand at 948.711 kb on - strand at 948.770 kb on + strand at 948.812 kb on + strand at 948.951 kb on + strand at 948.959 kb on - strand at 948.959 kb on - strand at 948.964 kb on - strand at 948.970 kb on + strand at 949.105 kb on - strand at 949.123 kb on - strand at 949.134 kb on - strand at 949.144 kb on - strand at 949.250 kb on - strand at 949.275 kb on - strand at 949.561 kb on + strand, within Dsui_0922 at 949.569 kb on - strand, within Dsui_0922 at 949.569 kb on - strand, within Dsui_0922 at 949.675 kb on + strand, within Dsui_0922 at 949.790 kb on - strand, within Dsui_0922 at 949.879 kb on + strand, within Dsui_0922 at 949.879 kb on + strand, within Dsui_0922 at 949.879 kb on + strand, within Dsui_0922 at 949.887 kb on - strand, within Dsui_0922 at 949.902 kb on - strand, within Dsui_0922 at 950.033 kb on - strand, within Dsui_0922 at 950.244 kb on + strand, within Dsui_0922 at 950.307 kb on - strand, within Dsui_0922 at 950.399 kb on - strand, within Dsui_0922 at 950.400 kb on - strand, within Dsui_0922 at 950.415 kb on + strand, within Dsui_0922 at 950.415 kb on + strand, within Dsui_0922 at 950.447 kb on - strand, within Dsui_0922 at 950.805 kb on + strand, within Dsui_0922 at 950.974 kb on + strand at 951.030 kb on - strand at 951.056 kb on + strand at 951.189 kb on - strand at 951.208 kb on - strand, within Dsui_0923 at 951.252 kb on - strand, within Dsui_0923 at 951.467 kb on - strand, within Dsui_0923 at 951.527 kb on + strand, within Dsui_0923 at 951.638 kb on - strand at 951.657 kb on - strand, within Dsui_0923 at 951.761 kb on - strand, within Dsui_0923 at 951.761 kb on - strand, within Dsui_0923 at 951.782 kb on - strand, within Dsui_0923 at 951.943 kb on - strand, within Dsui_0923 at 952.022 kb on + strand, within Dsui_0923 at 952.027 kb on + strand at 952.027 kb on + strand, within Dsui_0923
Per-strain Table
Position Strand Gene LocusTag Fraction ALP with Fusidic 0.02 mg/ml remove 948,018 + +0.5 948,146 - Dsui_0921 0.82 +0.5 948,150 + Dsui_0921 0.82 -1.3 948,203 - Dsui_0921 0.84 +2.8 948,299 + Dsui_0921 0.86 -1.8 948,317 - Dsui_0921 0.86 -1.5 948,451 + Dsui_0921 0.89 -0.3 948,616 + -1.8 948,616 + -0.1 948,624 - -2.8 948,678 + +0.5 948,693 - +0.5 948,711 - -0.1 948,770 + -1.8 948,812 + +0.5 948,951 + -1.8 948,959 - -1.5 948,959 - +0.5 948,964 - -0.5 948,970 + +1.5 949,105 - +0.5 949,123 - +0.5 949,134 - -2.3 949,144 - +0.5 949,250 - +0.5 949,275 - +0.5 949,561 + Dsui_0922 0.27 -2.3 949,569 - Dsui_0922 0.28 -0.5 949,569 - Dsui_0922 0.28 -1.5 949,675 + Dsui_0922 0.33 -1.1 949,790 - Dsui_0922 0.39 -1.1 949,879 + Dsui_0922 0.43 -0.5 949,879 + Dsui_0922 0.43 -0.5 949,879 + Dsui_0922 0.43 +0.5 949,887 - Dsui_0922 0.44 +0.5 949,902 - Dsui_0922 0.44 +0.5 950,033 - Dsui_0922 0.51 -1.1 950,244 + Dsui_0922 0.61 -1.5 950,307 - Dsui_0922 0.64 -0.5 950,399 - Dsui_0922 0.69 +0.5 950,400 - Dsui_0922 0.69 +0.5 950,415 + Dsui_0922 0.70 -1.3 950,415 + Dsui_0922 0.70 +2.1 950,447 - Dsui_0922 0.71 +0.5 950,805 + Dsui_0922 0.89 -0.5 950,974 + -0.5 951,030 - +0.5 951,056 + +2.5 951,189 - +0.5 951,208 - Dsui_0923 0.13 -1.8 951,252 - Dsui_0923 0.17 +0.5 951,467 - Dsui_0923 0.36 -1.1 951,527 + Dsui_0923 0.41 -1.5 951,638 - +0.5 951,657 - Dsui_0923 0.52 +0.5 951,761 - Dsui_0923 0.61 -1.1 951,761 - Dsui_0923 0.61 -1.5 951,782 - Dsui_0923 0.63 -1.8 951,943 - Dsui_0923 0.77 -0.2 952,022 + Dsui_0923 0.84 -1.8 952,027 + +0.5 952,027 + Dsui_0923 0.84 -0.5
Or see this region's nucleotide sequence