Experiment: ALP with Fusidic 0.02 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Dsui_0792 and Dsui_0793 are separated by 22 nucleotides Dsui_0793 and Dsui_0794 are separated by 49 nucleotides
Dsui_0792: Dsui_0792 - hypothetical protein, at 799,492 to 799,980
_0792
Dsui_0793: Dsui_0793 - N-acetylmuramoyl-L-alanine amidase, at 800,003 to 800,746
_0793
Dsui_0794: Dsui_0794 - putative protease with the C-terminal PDZ domain, at 800,796 to 802,634
_0794
Position (kb)
800
801 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 799.020 kb on + strand at 799.031 kb on - strand at 799.031 kb on - strand at 799.033 kb on - strand at 799.044 kb on - strand at 799.047 kb on - strand at 799.051 kb on + strand at 799.051 kb on + strand at 799.051 kb on + strand at 799.054 kb on - strand at 799.057 kb on + strand at 799.059 kb on - strand at 799.065 kb on - strand at 799.065 kb on - strand at 799.069 kb on + strand at 799.077 kb on + strand at 799.085 kb on - strand at 799.095 kb on - strand at 799.096 kb on + strand at 799.096 kb on + strand at 799.096 kb on + strand at 799.096 kb on + strand at 799.096 kb on + strand at 799.096 kb on + strand at 799.096 kb on + strand at 799.096 kb on - strand at 799.104 kb on - strand at 799.109 kb on - strand at 799.109 kb on - strand at 799.111 kb on - strand at 799.114 kb on - strand at 799.121 kb on + strand at 799.129 kb on - strand at 799.139 kb on - strand at 799.141 kb on + strand at 799.306 kb on + strand at 799.311 kb on + strand at 799.561 kb on - strand, within Dsui_0792 at 799.567 kb on - strand, within Dsui_0792 at 799.655 kb on - strand, within Dsui_0792 at 799.760 kb on - strand, within Dsui_0792 at 799.760 kb on - strand, within Dsui_0792 at 799.790 kb on - strand, within Dsui_0792 at 799.791 kb on - strand, within Dsui_0792 at 799.854 kb on - strand, within Dsui_0792 at 799.894 kb on + strand, within Dsui_0792 at 799.895 kb on - strand, within Dsui_0792 at 799.989 kb on + strand at 800.051 kb on - strand at 800.170 kb on + strand, within Dsui_0793 at 800.245 kb on - strand, within Dsui_0793 at 800.245 kb on - strand, within Dsui_0793 at 800.309 kb on + strand, within Dsui_0793 at 800.357 kb on - strand, within Dsui_0793 at 800.394 kb on + strand, within Dsui_0793 at 800.399 kb on + strand, within Dsui_0793 at 800.407 kb on - strand, within Dsui_0793 at 800.412 kb on - strand, within Dsui_0793 at 800.449 kb on - strand, within Dsui_0793 at 800.499 kb on + strand, within Dsui_0793 at 800.555 kb on + strand, within Dsui_0793 at 800.556 kb on - strand, within Dsui_0793 at 800.562 kb on - strand, within Dsui_0793 at 800.591 kb on - strand, within Dsui_0793 at 800.601 kb on - strand, within Dsui_0793 at 800.783 kb on - strand at 800.783 kb on - strand at 800.873 kb on + strand at 800.880 kb on + strand at 800.890 kb on + strand at 800.899 kb on + strand at 800.919 kb on + strand at 800.920 kb on + strand at 800.928 kb on - strand at 800.930 kb on + strand at 800.943 kb on + strand at 800.951 kb on - strand at 800.951 kb on - strand at 800.993 kb on + strand, within Dsui_0794 at 801.027 kb on - strand, within Dsui_0794 at 801.046 kb on - strand, within Dsui_0794 at 801.103 kb on - strand, within Dsui_0794 at 801.103 kb on - strand, within Dsui_0794 at 801.129 kb on + strand, within Dsui_0794 at 801.193 kb on - strand, within Dsui_0794 at 801.225 kb on + strand, within Dsui_0794 at 801.244 kb on - strand at 801.244 kb on - strand, within Dsui_0794 at 801.323 kb on - strand, within Dsui_0794 at 801.325 kb on - strand, within Dsui_0794 at 801.445 kb on + strand, within Dsui_0794 at 801.456 kb on + strand, within Dsui_0794 at 801.458 kb on + strand, within Dsui_0794 at 801.458 kb on - strand at 801.458 kb on - strand, within Dsui_0794 at 801.580 kb on + strand at 801.603 kb on + strand, within Dsui_0794 at 801.603 kb on + strand, within Dsui_0794 at 801.611 kb on - strand, within Dsui_0794 at 801.613 kb on + strand, within Dsui_0794 at 801.616 kb on - strand, within Dsui_0794
Per-strain Table
Position Strand Gene LocusTag Fraction ALP with Fusidic 0.02 mg/ml remove 799,020 + -1.8 799,031 - -1.8 799,031 - +0.5 799,033 - -0.5 799,044 - +0.5 799,047 - -0.5 799,051 + +0.5 799,051 + -0.5 799,051 + +0.5 799,054 - -1.1 799,057 + -1.5 799,059 - -1.1 799,065 - -1.5 799,065 - +0.5 799,069 + +0.5 799,077 + +0.5 799,085 - -0.5 799,095 - -1.1 799,096 + +0.5 799,096 + +0.5 799,096 + -1.5 799,096 + -0.5 799,096 + +0.5 799,096 + -1.1 799,096 + +0.5 799,096 - +0.5 799,104 - -0.1 799,109 - -1.1 799,109 - -1.1 799,111 - +0.6 799,114 - -1.1 799,121 + -0.5 799,129 - -0.5 799,139 - +0.5 799,141 + -1.8 799,306 + +0.5 799,311 + +0.5 799,561 - Dsui_0792 0.14 -1.5 799,567 - Dsui_0792 0.15 -2.1 799,655 - Dsui_0792 0.33 +0.5 799,760 - Dsui_0792 0.55 -1.1 799,760 - Dsui_0792 0.55 -0.3 799,790 - Dsui_0792 0.61 +0.1 799,791 - Dsui_0792 0.61 -0.5 799,854 - Dsui_0792 0.74 +0.5 799,894 + Dsui_0792 0.82 -1.5 799,895 - Dsui_0792 0.82 +0.3 799,989 + +0.5 800,051 - -0.2 800,170 + Dsui_0793 0.22 -1.1 800,245 - Dsui_0793 0.33 -0.5 800,245 - Dsui_0793 0.33 -0.7 800,309 + Dsui_0793 0.41 -0.5 800,357 - Dsui_0793 0.48 +0.3 800,394 + Dsui_0793 0.53 -0.5 800,399 + Dsui_0793 0.53 +0.5 800,407 - Dsui_0793 0.54 -0.4 800,412 - Dsui_0793 0.55 +0.1 800,449 - Dsui_0793 0.60 -1.9 800,499 + Dsui_0793 0.67 -0.5 800,555 + Dsui_0793 0.74 -1.1 800,556 - Dsui_0793 0.74 -1.3 800,562 - Dsui_0793 0.75 -1.5 800,591 - Dsui_0793 0.79 +0.5 800,601 - Dsui_0793 0.80 +1.0 800,783 - -1.8 800,783 - +0.3 800,873 + -0.2 800,880 + -1.9 800,890 + -0.1 800,899 + +0.1 800,919 + -2.8 800,920 + +1.6 800,928 - +0.5 800,930 + +0.1 800,943 + +0.8 800,951 - +0.5 800,951 - +0.3 800,993 + Dsui_0794 0.11 -0.5 801,027 - Dsui_0794 0.13 +0.5 801,046 - Dsui_0794 0.14 -1.1 801,103 - Dsui_0794 0.17 -0.5 801,103 - Dsui_0794 0.17 -0.5 801,129 + Dsui_0794 0.18 +0.5 801,193 - Dsui_0794 0.22 -2.3 801,225 + Dsui_0794 0.23 +1.1 801,244 - -0.5 801,244 - Dsui_0794 0.24 -0.5 801,323 - Dsui_0794 0.29 -1.5 801,325 - Dsui_0794 0.29 -0.5 801,445 + Dsui_0794 0.35 -0.6 801,456 + Dsui_0794 0.36 -0.8 801,458 + Dsui_0794 0.36 -0.7 801,458 - -0.5 801,458 - Dsui_0794 0.36 +0.5 801,580 + +0.9 801,603 + Dsui_0794 0.44 -1.1 801,603 + Dsui_0794 0.44 -3.6 801,611 - Dsui_0794 0.44 +0.5 801,613 + Dsui_0794 0.44 -1.1 801,616 - Dsui_0794 0.45 +0.5
Or see this region's nucleotide sequence