Experiment: ALP with Fusidic 0.02 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Dsui_0736 and Dsui_0737 are separated by 82 nucleotides Dsui_0737 and Dsui_0738 are separated by 14 nucleotides Dsui_0738 and Dsui_0739 are separated by 15 nucleotides Dsui_0739 and Dsui_0740 are separated by 2 nucleotides Dsui_0740 and Dsui_0741 are separated by 10 nucleotides
Dsui_0736: Dsui_0736 - FAD/FMN-dependent dehydrogenase, at 732,078 to 735,956
_0736
Dsui_0737: Dsui_0737 - hypothetical protein, at 736,039 to 736,134
_0737
Dsui_0738: Dsui_0738 - secondary thiamine-phosphate synthase enzyme, at 736,149 to 736,568
_0738
Dsui_0739: Dsui_0739 - Major Facilitator Superfamily transporter, at 736,584 to 738,125
_0739
Dsui_0740: Dsui_0740 - methionine biosynthesis protein MetW, at 738,128 to 738,724
_0740
Dsui_0741: Dsui_0741 - homoserine O-acetyltransferase, at 738,735 to 739,862
_0741
Position (kb) 736
737
738
739 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 736.051 kb on - strand, within Dsui_0737 at 736.063 kb on + strand, within Dsui_0737 at 736.107 kb on + strand, within Dsui_0737 at 736.129 kb on + strand at 736.191 kb on - strand, within Dsui_0738 at 736.506 kb on - strand, within Dsui_0738 at 736.572 kb on - strand at 736.572 kb on - strand at 736.692 kb on + strand at 736.837 kb on - strand, within Dsui_0739 at 736.980 kb on - strand, within Dsui_0739 at 737.049 kb on + strand, within Dsui_0739 at 737.054 kb on + strand, within Dsui_0739 at 737.057 kb on - strand, within Dsui_0739 at 737.062 kb on - strand, within Dsui_0739 at 737.065 kb on + strand, within Dsui_0739 at 737.150 kb on - strand, within Dsui_0739 at 737.169 kb on - strand, within Dsui_0739 at 737.217 kb on - strand, within Dsui_0739 at 737.238 kb on + strand, within Dsui_0739 at 737.319 kb on + strand, within Dsui_0739 at 737.327 kb on - strand, within Dsui_0739 at 737.379 kb on - strand, within Dsui_0739 at 737.392 kb on - strand, within Dsui_0739 at 737.420 kb on - strand, within Dsui_0739 at 737.421 kb on + strand at 737.493 kb on + strand, within Dsui_0739 at 737.611 kb on + strand at 737.619 kb on - strand, within Dsui_0739 at 737.623 kb on - strand, within Dsui_0739 at 737.629 kb on - strand, within Dsui_0739 at 737.849 kb on - strand, within Dsui_0739 at 737.891 kb on + strand at 737.901 kb on + strand, within Dsui_0739 at 737.909 kb on - strand, within Dsui_0739 at 737.990 kb on + strand at 738.050 kb on - strand at 738.056 kb on - strand at 738.126 kb on + strand at 738.139 kb on - strand at 738.139 kb on - strand at 738.139 kb on - strand at 738.187 kb on + strand at 738.310 kb on + strand, within Dsui_0740 at 738.347 kb on - strand, within Dsui_0740 at 738.399 kb on - strand, within Dsui_0740 at 738.458 kb on + strand at 738.508 kb on + strand, within Dsui_0740 at 738.508 kb on + strand, within Dsui_0740 at 738.508 kb on + strand, within Dsui_0740 at 738.523 kb on + strand, within Dsui_0740 at 738.559 kb on - strand, within Dsui_0740 at 738.559 kb on - strand, within Dsui_0740 at 738.559 kb on - strand, within Dsui_0740 at 738.559 kb on - strand, within Dsui_0740 at 738.580 kb on + strand, within Dsui_0740 at 738.649 kb on - strand, within Dsui_0740
Per-strain Table
Position Strand Gene LocusTag Fraction ALP with Fusidic 0.02 mg/ml remove 736,051 - Dsui_0737 0.12 +0.4 736,063 + Dsui_0737 0.25 -0.3 736,107 + Dsui_0737 0.71 -0.2 736,129 + -0.2 736,191 - Dsui_0738 0.10 -1.6 736,506 - Dsui_0738 0.85 -1.4 736,572 - -0.6 736,572 - -1.0 736,692 + +0.4 736,837 - Dsui_0739 0.16 -1.4 736,980 - Dsui_0739 0.26 -0.3 737,049 + Dsui_0739 0.30 -1.2 737,054 + Dsui_0739 0.30 +0.4 737,057 - Dsui_0739 0.31 -0.9 737,062 - Dsui_0739 0.31 -1.7 737,065 + Dsui_0739 0.31 -2.6 737,150 - Dsui_0739 0.37 +0.5 737,169 - Dsui_0739 0.38 -2.2 737,217 - Dsui_0739 0.41 -0.9 737,238 + Dsui_0739 0.42 -1.2 737,319 + Dsui_0739 0.48 +0.4 737,327 - Dsui_0739 0.48 -2.6 737,379 - Dsui_0739 0.52 -2.0 737,392 - Dsui_0739 0.52 -1.0 737,420 - Dsui_0739 0.54 -0.6 737,421 + -0.6 737,493 + Dsui_0739 0.59 -1.9 737,611 + +0.4 737,619 - Dsui_0739 0.67 -1.5 737,623 - Dsui_0739 0.67 -3.1 737,629 - Dsui_0739 0.68 -1.7 737,849 - Dsui_0739 0.82 -1.8 737,891 + +0.4 737,901 + Dsui_0739 0.85 -1.9 737,909 - Dsui_0739 0.86 -0.5 737,990 + -1.2 738,050 - +0.7 738,056 - -0.8 738,126 + +0.4 738,139 - -1.5 738,139 - -2.1 738,139 - -2.6 738,187 + -1.2 738,310 + Dsui_0740 0.30 -1.9 738,347 - Dsui_0740 0.37 -0.4 738,399 - Dsui_0740 0.45 -1.7 738,458 + +0.4 738,508 + Dsui_0740 0.64 +1.4 738,508 + Dsui_0740 0.64 -1.2 738,508 + Dsui_0740 0.64 +0.4 738,523 + Dsui_0740 0.66 -1.2 738,559 - Dsui_0740 0.72 +0.0 738,559 - Dsui_0740 0.72 -0.8 738,559 - Dsui_0740 0.72 -0.3 738,559 - Dsui_0740 0.72 -0.8 738,580 + Dsui_0740 0.76 -0.6 738,649 - Dsui_0740 0.87 -1.1
Or see this region's nucleotide sequence