Experiment: ALP with Fusidic 0.02 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Dsui_0701 and Dsui_0702 overlap by 32 nucleotides Dsui_0702 and Dsui_0703 are separated by 155 nucleotides Dsui_0703 and Dsui_0704 are separated by 14 nucleotides Dsui_0704 and Dsui_0705 are separated by 66 nucleotides Dsui_0705 and Dsui_0706 are separated by 65 nucleotides
Dsui_0701: Dsui_0701 - serine kinase of the HPr protein, regulates carbohydrate metabolism, at 696,888 to 697,823
_0701
Dsui_0702: Dsui_0702 - PTS IIA-like nitrogen-regulatory protein PtsN, at 697,792 to 698,277
_0702
Dsui_0703: Dsui_0703 - ribosomal subunit interface protein, at 698,433 to 698,753
_0703
Dsui_0704: Dsui_0704 - RNA polymerase sigma-54 factor, at 698,768 to 700,246
_0704
Dsui_0705: Dsui_0705 - ABC-type (unclassified) transport system, ATPase component, at 700,313 to 701,059
_0705
Dsui_0706: Dsui_0706 - hypothetical protein, at 701,125 to 701,580
_0706
Position (kb) 698
699
700
701 Strain fitness (log2 ratio) -1
0
1
2 at 698.421 kb on - strand at 698.479 kb on - strand, within Dsui_0703 at 698.493 kb on + strand, within Dsui_0703 at 698.613 kb on - strand, within Dsui_0703 at 698.613 kb on - strand, within Dsui_0703 at 698.640 kb on - strand, within Dsui_0703 at 698.647 kb on - strand, within Dsui_0703 at 698.676 kb on - strand, within Dsui_0703 at 698.719 kb on + strand, within Dsui_0703 at 698.802 kb on - strand at 698.816 kb on + strand at 698.837 kb on + strand at 698.847 kb on - strand at 698.850 kb on - strand at 699.035 kb on - strand, within Dsui_0704 at 699.035 kb on - strand, within Dsui_0704 at 699.058 kb on + strand, within Dsui_0704 at 699.081 kb on - strand, within Dsui_0704 at 699.081 kb on - strand, within Dsui_0704 at 699.081 kb on - strand, within Dsui_0704 at 699.226 kb on + strand at 699.356 kb on - strand, within Dsui_0704 at 699.417 kb on - strand, within Dsui_0704 at 699.442 kb on - strand, within Dsui_0704 at 699.493 kb on + strand, within Dsui_0704 at 699.511 kb on - strand, within Dsui_0704 at 699.609 kb on - strand, within Dsui_0704 at 699.678 kb on - strand, within Dsui_0704 at 699.683 kb on - strand, within Dsui_0704 at 699.709 kb on - strand, within Dsui_0704 at 699.928 kb on + strand, within Dsui_0704 at 699.928 kb on + strand, within Dsui_0704 at 699.967 kb on + strand, within Dsui_0704 at 700.040 kb on - strand, within Dsui_0704 at 700.200 kb on - strand at 700.212 kb on - strand at 700.282 kb on + strand at 700.302 kb on + strand at 701.087 kb on - strand at 701.100 kb on + strand at 701.109 kb on - strand at 701.135 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction ALP with Fusidic 0.02 mg/ml remove 698,421 - -0.4 698,479 - Dsui_0703 0.14 -1.6 698,493 + Dsui_0703 0.19 -0.6 698,613 - Dsui_0703 0.56 -0.1 698,613 - Dsui_0703 0.56 -0.1 698,640 - Dsui_0703 0.64 -0.9 698,647 - Dsui_0703 0.67 -0.6 698,676 - Dsui_0703 0.76 -0.7 698,719 + Dsui_0703 0.89 -0.9 698,802 - +1.0 698,816 + +0.4 698,837 + +1.7 698,847 - +1.6 698,850 - +0.7 699,035 - Dsui_0704 0.18 +1.5 699,035 - Dsui_0704 0.18 +1.1 699,058 + Dsui_0704 0.20 +1.4 699,081 - Dsui_0704 0.21 +2.0 699,081 - Dsui_0704 0.21 +1.1 699,081 - Dsui_0704 0.21 +1.2 699,226 + +0.4 699,356 - Dsui_0704 0.40 +1.4 699,417 - Dsui_0704 0.44 +0.4 699,442 - Dsui_0704 0.46 +1.5 699,493 + Dsui_0704 0.49 +0.7 699,511 - Dsui_0704 0.50 +1.0 699,609 - Dsui_0704 0.57 +0.8 699,678 - Dsui_0704 0.62 +1.0 699,683 - Dsui_0704 0.62 +0.7 699,709 - Dsui_0704 0.64 +1.0 699,928 + Dsui_0704 0.78 +0.8 699,928 + Dsui_0704 0.78 -0.9 699,967 + Dsui_0704 0.81 +1.9 700,040 - Dsui_0704 0.86 +1.1 700,200 - +1.1 700,212 - +0.9 700,282 + +1.5 700,302 + +0.5 701,087 - -0.4 701,100 + -1.6 701,109 - -0.6 701,135 + -0.6
Or see this region's nucleotide sequence