Experiment: ALP with Fusidic 0.02 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Dsui_0659 and Dsui_0660 are separated by 21 nucleotides Dsui_0660 and Dsui_0661 are separated by 90 nucleotides Dsui_0661 and Dsui_0662 are separated by 91 nucleotides Dsui_0662 and Dsui_0663 overlap by 8 nucleotides Dsui_0663 and Dsui_0664 overlap by 14 nucleotides
Dsui_0659: Dsui_0659 - response regulator with CheY-like receiver domain and winged-helix DNA-binding domain, at 658,093 to 658,455
_0659
Dsui_0660: Dsui_0660 - response regulator with CheY-like receiver domain and winged-helix DNA-binding domain, at 658,477 to 658,875
_0660
Dsui_0661: Dsui_0661 - rubredoxin, at 658,966 to 659,166
_0661
Dsui_0662: Dsui_0662 - phosphomethylpyrimidine kinase, at 659,258 to 660,100
_0662
Dsui_0663: Dsui_0663 - thiamine-phosphate pyrophosphorylase, at 660,093 to 660,740
_0663
Dsui_0664: Dsui_0664 - glutamate-1-semialdehyde-2,1-aminomutase, at 660,727 to 662,010
_0664
Position (kb) 659
660
661 Strain fitness (log2 ratio) -2
-1
0
1
2
3
4 at 658.316 kb on - strand, within Dsui_0659 at 658.328 kb on + strand at 658.457 kb on + strand at 658.458 kb on - strand at 658.459 kb on - strand at 658.486 kb on - strand at 658.496 kb on - strand at 658.554 kb on + strand, within Dsui_0660 at 658.562 kb on - strand, within Dsui_0660 at 658.582 kb on - strand, within Dsui_0660 at 658.619 kb on + strand at 658.738 kb on - strand, within Dsui_0660 at 658.772 kb on - strand, within Dsui_0660 at 658.850 kb on - strand at 658.860 kb on - strand at 658.896 kb on + strand at 658.904 kb on - strand at 658.910 kb on - strand at 658.914 kb on - strand at 659.012 kb on + strand, within Dsui_0661 at 659.150 kb on + strand at 659.156 kb on + strand at 659.174 kb on - strand at 659.174 kb on - strand at 659.242 kb on - strand at 659.297 kb on + strand at 660.104 kb on + strand at 660.723 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction ALP with Fusidic 0.02 mg/ml remove 658,316 - Dsui_0659 0.61 +4.6 658,328 + +0.4 658,457 + +3.0 658,458 - +3.3 658,459 - +3.0 658,486 - +2.0 658,496 - +0.7 658,554 + Dsui_0660 0.19 +0.4 658,562 - Dsui_0660 0.21 +3.1 658,582 - Dsui_0660 0.26 +2.7 658,619 + +0.4 658,738 - Dsui_0660 0.65 +3.3 658,772 - Dsui_0660 0.74 +2.4 658,850 - +2.0 658,860 - +1.6 658,896 + +0.4 658,904 - +2.4 658,910 - +0.2 658,914 - +1.4 659,012 + Dsui_0661 0.23 +0.4 659,150 + +0.4 659,156 + -0.6 659,174 - -1.8 659,174 - -0.6 659,242 - -1.6 659,297 + +1.2 660,104 + -0.9 660,723 + +1.2
Or see this region's nucleotide sequence