Experiment: ALP with Fusidic 0.02 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Dsui_0544 and Dsui_0545 are separated by 94 nucleotides Dsui_0545 and Dsui_0546 are separated by 81 nucleotides Dsui_0546 and Dsui_0547 are separated by 60 nucleotides Dsui_0547 and Dsui_0548 are separated by 72 nucleotides Dsui_0548 and Dsui_0549 overlap by 1 nucleotides Dsui_0549 and Dsui_0550 are separated by 240 nucleotides
Dsui_0544: Dsui_0544 - flagellar basal body-associated protein, at 550,335 to 550,724
_0544
Dsui_0545: Dsui_0545 - tRNA-Thr, at 550,819 to 550,893
_0545
Dsui_0546: Dsui_0546 - tRNA-Lys, at 550,975 to 551,050
_0546
Dsui_0547: Dsui_0547 - tRNA-Lys, at 551,111 to 551,186
_0547
Dsui_0548: Dsui_0548 - putative transporter component, at 551,259 to 552,365
_0548
Dsui_0549: Dsui_0549 - queuosine biosynthesis protein QueC, at 552,365 to 553,054
_0549
Dsui_0550: Dsui_0550 - nitrogenase molybdenum-iron cofactor biosynthesis protein NifE, at 553,295 to 554,752
_0550
Position (kb) 551
552
553 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 550.301 kb on + strand at 550.378 kb on + strand, within Dsui_0544 at 550.385 kb on - strand, within Dsui_0544 at 550.508 kb on + strand, within Dsui_0544 at 550.689 kb on + strand at 550.699 kb on + strand at 550.707 kb on - strand at 550.738 kb on - strand at 550.751 kb on - strand at 550.754 kb on + strand at 550.799 kb on - strand at 550.806 kb on - strand at 550.809 kb on + strand at 551.340 kb on + strand at 551.391 kb on - strand, within Dsui_0548 at 551.529 kb on - strand, within Dsui_0548 at 551.585 kb on + strand at 551.616 kb on + strand, within Dsui_0548 at 551.943 kb on - strand, within Dsui_0548 at 552.007 kb on - strand, within Dsui_0548 at 552.127 kb on - strand, within Dsui_0548 at 552.191 kb on - strand, within Dsui_0548 at 552.306 kb on - strand at 552.487 kb on + strand at 552.551 kb on + strand, within Dsui_0549 at 552.559 kb on - strand, within Dsui_0549 at 552.591 kb on - strand, within Dsui_0549 at 552.619 kb on + strand at 552.702 kb on + strand at 552.705 kb on - strand, within Dsui_0549 at 552.717 kb on + strand at 552.717 kb on + strand at 552.730 kb on - strand, within Dsui_0549 at 552.745 kb on - strand, within Dsui_0549 at 552.768 kb on - strand, within Dsui_0549 at 552.808 kb on + strand, within Dsui_0549 at 552.862 kb on - strand at 552.955 kb on + strand at 553.058 kb on - strand at 553.068 kb on - strand at 553.114 kb on + strand at 553.192 kb on + strand at 553.219 kb on + strand at 553.219 kb on + strand at 553.227 kb on - strand at 553.229 kb on + strand at 553.231 kb on + strand at 553.357 kb on + strand at 553.363 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction ALP with Fusidic 0.02 mg/ml remove 550,301 + -0.6 550,378 + Dsui_0544 0.11 -0.6 550,385 - Dsui_0544 0.13 +0.4 550,508 + Dsui_0544 0.44 -0.8 550,689 + -3.4 550,699 + +0.4 550,707 - +0.0 550,738 - -0.2 550,751 - -0.4 550,754 + -0.2 550,799 - +0.4 550,806 - -0.2 550,809 + -1.2 551,340 + +0.4 551,391 - Dsui_0548 0.12 -0.1 551,529 - Dsui_0548 0.24 +0.8 551,585 + +0.4 551,616 + Dsui_0548 0.32 +0.4 551,943 - Dsui_0548 0.62 -0.2 552,007 - Dsui_0548 0.68 -0.2 552,127 - Dsui_0548 0.78 -0.2 552,191 - Dsui_0548 0.84 +1.9 552,306 - +0.2 552,487 + +0.4 552,551 + Dsui_0549 0.27 -1.6 552,559 - Dsui_0549 0.28 +0.3 552,591 - Dsui_0549 0.33 +0.6 552,619 + -0.6 552,702 + +0.4 552,705 - Dsui_0549 0.49 +2.2 552,717 + -1.2 552,717 + +0.4 552,730 - Dsui_0549 0.53 +0.2 552,745 - Dsui_0549 0.55 +1.4 552,768 - Dsui_0549 0.58 -0.2 552,808 + Dsui_0549 0.64 +0.4 552,862 - +0.4 552,955 + -0.6 553,058 - +0.7 553,068 - +0.4 553,114 + -1.9 553,192 + +0.4 553,219 + +0.4 553,219 + -0.6 553,227 - +0.4 553,229 + -0.6 553,231 + -2.9 553,357 + +0.4 553,363 + +0.4
Or see this region's nucleotide sequence