Experiment: ALP with Fusidic 0.02 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Dsui_0542 and Dsui_0543 are separated by 228 nucleotides Dsui_0543 and Dsui_0544 are separated by 124 nucleotides Dsui_0544 and Dsui_0545 are separated by 94 nucleotides Dsui_0545 and Dsui_0546 are separated by 81 nucleotides Dsui_0546 and Dsui_0547 are separated by 60 nucleotides
Dsui_0542: Dsui_0542 - cobyric acid synthase CobQ, at 546,212 to 547,705
_0542
Dsui_0543: Dsui_0543 - malic enzyme, at 547,934 to 550,210
_0543
Dsui_0544: Dsui_0544 - flagellar basal body-associated protein, at 550,335 to 550,724
_0544
Dsui_0545: Dsui_0545 - tRNA-Thr, at 550,819 to 550,893
_0545
Dsui_0546: Dsui_0546 - tRNA-Lys, at 550,975 to 551,050
_0546
Dsui_0547: Dsui_0547 - tRNA-Lys, at 551,111 to 551,186
_0547
Position (kb) 547
548
549
550
551 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 546.950 kb on + strand, within Dsui_0542 at 546.970 kb on + strand, within Dsui_0542 at 547.002 kb on + strand at 547.049 kb on + strand, within Dsui_0542 at 547.131 kb on - strand, within Dsui_0542 at 547.262 kb on + strand, within Dsui_0542 at 547.374 kb on - strand, within Dsui_0542 at 547.424 kb on + strand, within Dsui_0542 at 547.432 kb on - strand, within Dsui_0542 at 547.622 kb on + strand at 547.918 kb on + strand at 547.918 kb on + strand at 547.924 kb on + strand at 547.925 kb on + strand at 547.926 kb on - strand at 548.015 kb on + strand at 548.071 kb on - strand at 548.092 kb on + strand at 548.098 kb on - strand at 548.100 kb on - strand at 548.100 kb on - strand at 548.172 kb on - strand, within Dsui_0543 at 548.307 kb on + strand, within Dsui_0543 at 548.482 kb on + strand, within Dsui_0543 at 548.593 kb on + strand, within Dsui_0543 at 548.613 kb on - strand, within Dsui_0543 at 548.613 kb on - strand at 548.688 kb on - strand, within Dsui_0543 at 548.778 kb on + strand, within Dsui_0543 at 548.796 kb on - strand, within Dsui_0543 at 548.871 kb on + strand, within Dsui_0543 at 548.979 kb on - strand at 549.313 kb on + strand, within Dsui_0543 at 549.334 kb on + strand, within Dsui_0543 at 549.355 kb on - strand, within Dsui_0543 at 549.393 kb on + strand, within Dsui_0543 at 549.552 kb on + strand, within Dsui_0543 at 549.810 kb on + strand, within Dsui_0543 at 549.810 kb on + strand, within Dsui_0543 at 549.818 kb on - strand, within Dsui_0543 at 549.901 kb on + strand, within Dsui_0543 at 549.963 kb on - strand, within Dsui_0543 at 550.219 kb on + strand at 550.227 kb on - strand at 550.232 kb on - strand at 550.301 kb on + strand at 550.378 kb on + strand, within Dsui_0544 at 550.385 kb on - strand, within Dsui_0544 at 550.508 kb on + strand, within Dsui_0544 at 550.689 kb on + strand at 550.699 kb on + strand at 550.707 kb on - strand at 550.738 kb on - strand at 550.751 kb on - strand at 550.754 kb on + strand at 550.799 kb on - strand at 550.806 kb on - strand at 550.809 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction ALP with Fusidic 0.02 mg/ml remove 546,950 + Dsui_0542 0.49 +0.4 546,970 + Dsui_0542 0.51 -0.2 547,002 + -1.2 547,049 + Dsui_0542 0.56 -1.6 547,131 - Dsui_0542 0.62 +0.4 547,262 + Dsui_0542 0.70 -0.2 547,374 - Dsui_0542 0.78 -1.9 547,424 + Dsui_0542 0.81 -0.8 547,432 - Dsui_0542 0.82 -3.0 547,622 + +1.0 547,918 + +1.0 547,918 + +1.3 547,924 + +0.1 547,925 + +2.6 547,926 - +0.4 548,015 + +1.4 548,071 - +0.1 548,092 + +1.6 548,098 - +0.4 548,100 - -1.9 548,100 - -0.2 548,172 - Dsui_0543 0.10 +0.4 548,307 + Dsui_0543 0.16 +1.0 548,482 + Dsui_0543 0.24 +1.7 548,593 + Dsui_0543 0.29 +1.6 548,613 - Dsui_0543 0.30 +1.2 548,613 - +0.4 548,688 - Dsui_0543 0.33 -0.2 548,778 + Dsui_0543 0.37 +1.6 548,796 - Dsui_0543 0.38 +1.5 548,871 + Dsui_0543 0.41 +1.4 548,979 - +1.6 549,313 + Dsui_0543 0.61 +1.8 549,334 + Dsui_0543 0.61 +1.3 549,355 - Dsui_0543 0.62 +2.3 549,393 + Dsui_0543 0.64 +1.1 549,552 + Dsui_0543 0.71 +1.7 549,810 + Dsui_0543 0.82 +1.9 549,810 + Dsui_0543 0.82 +1.5 549,818 - Dsui_0543 0.83 +1.2 549,901 + Dsui_0543 0.86 +1.4 549,963 - Dsui_0543 0.89 +1.2 550,219 + +0.5 550,227 - -0.1 550,232 - -1.2 550,301 + -0.6 550,378 + Dsui_0544 0.11 -0.6 550,385 - Dsui_0544 0.13 +0.4 550,508 + Dsui_0544 0.44 -0.8 550,689 + -3.4 550,699 + +0.4 550,707 - +0.0 550,738 - -0.2 550,751 - -0.4 550,754 + -0.2 550,799 - +0.4 550,806 - -0.2 550,809 + -1.2
Or see this region's nucleotide sequence