Experiment: ALP with Fusidic 0.02 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Dsui_0519 and Dsui_0520 are separated by 330 nucleotides Dsui_0520 and Dsui_0521 are separated by 75 nucleotides Dsui_0521 and Dsui_0522 are separated by 94 nucleotides
Dsui_0519: Dsui_0519 - Methylmalonyl-CoA mutase (EC 5.4.99.2) <small>(from data)</small>, at 525,068 to 527,233
_0519
Dsui_0520: Dsui_0520 - transcriptional regulator, at 527,564 to 528,220
_0520
Dsui_0521: Dsui_0521 - enoyl-CoA hydratase/carnithine racemase, at 528,296 to 529,072
_0521
Dsui_0522: Dsui_0522 - SSS sodium solute transporter, at 529,167 to 530,840
_0522
Position (kb) 527
528
529 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1 at 526.572 kb on + strand, within Dsui_0519 at 526.613 kb on - strand, within Dsui_0519 at 526.638 kb on - strand, within Dsui_0519 at 526.666 kb on + strand, within Dsui_0519 at 526.843 kb on + strand, within Dsui_0519 at 526.843 kb on + strand, within Dsui_0519 at 526.881 kb on - strand, within Dsui_0519 at 526.890 kb on - strand, within Dsui_0519 at 526.983 kb on + strand at 527.026 kb on + strand at 527.060 kb on + strand at 527.063 kb on - strand at 527.129 kb on - strand at 527.169 kb on + strand at 527.177 kb on - strand at 527.177 kb on - strand at 527.233 kb on + strand at 527.234 kb on - strand at 527.241 kb on - strand at 527.247 kb on + strand at 527.302 kb on - strand at 527.511 kb on - strand at 527.563 kb on + strand at 527.674 kb on + strand, within Dsui_0520 at 527.720 kb on + strand, within Dsui_0520 at 527.754 kb on + strand, within Dsui_0520 at 527.791 kb on + strand, within Dsui_0520 at 527.791 kb on + strand, within Dsui_0520 at 527.883 kb on + strand, within Dsui_0520 at 527.952 kb on + strand, within Dsui_0520 at 527.953 kb on + strand, within Dsui_0520 at 527.960 kb on - strand, within Dsui_0520 at 527.960 kb on - strand, within Dsui_0520 at 528.101 kb on + strand, within Dsui_0520 at 528.168 kb on - strand at 528.194 kb on + strand at 528.205 kb on + strand at 528.207 kb on - strand at 528.347 kb on - strand at 528.347 kb on - strand at 528.429 kb on + strand, within Dsui_0521 at 528.442 kb on - strand, within Dsui_0521 at 528.530 kb on + strand, within Dsui_0521 at 528.594 kb on + strand, within Dsui_0521 at 528.596 kb on - strand, within Dsui_0521 at 528.599 kb on + strand, within Dsui_0521 at 528.692 kb on + strand, within Dsui_0521 at 528.827 kb on + strand, within Dsui_0521 at 528.846 kb on - strand, within Dsui_0521 at 528.854 kb on + strand, within Dsui_0521 at 528.856 kb on + strand, within Dsui_0521 at 528.940 kb on + strand, within Dsui_0521 at 528.967 kb on - strand, within Dsui_0521 at 528.967 kb on - strand, within Dsui_0521 at 529.045 kb on + strand at 529.055 kb on + strand at 529.123 kb on - strand at 529.200 kb on + strand at 529.200 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction ALP with Fusidic 0.02 mg/ml remove 526,572 + Dsui_0519 0.69 -1.6 526,613 - Dsui_0519 0.71 +0.9 526,638 - Dsui_0519 0.72 +0.7 526,666 + Dsui_0519 0.74 -0.6 526,843 + Dsui_0519 0.82 +0.4 526,843 + Dsui_0519 0.82 +0.4 526,881 - Dsui_0519 0.84 +1.1 526,890 - Dsui_0519 0.84 +0.5 526,983 + +0.4 527,026 + +0.4 527,060 + -1.9 527,063 - +1.0 527,129 - -1.0 527,169 + +0.4 527,177 - +0.3 527,177 - +0.4 527,233 + -0.6 527,234 - +0.1 527,241 - +1.4 527,247 + -1.6 527,302 - -0.1 527,511 - +0.4 527,563 + -0.7 527,674 + Dsui_0520 0.17 -3.8 527,720 + Dsui_0520 0.24 -4.2 527,754 + Dsui_0520 0.29 -1.8 527,791 + Dsui_0520 0.35 -4.3 527,791 + Dsui_0520 0.35 -4.7 527,883 + Dsui_0520 0.49 -3.3 527,952 + Dsui_0520 0.59 -3.7 527,953 + Dsui_0520 0.59 -4.0 527,960 - Dsui_0520 0.60 +0.4 527,960 - Dsui_0520 0.60 +0.4 528,101 + Dsui_0520 0.82 -4.0 528,168 - -1.2 528,194 + -2.7 528,205 + -0.7 528,207 - +0.4 528,347 - -2.9 528,347 - -0.6 528,429 + Dsui_0521 0.17 -0.6 528,442 - Dsui_0521 0.19 -1.2 528,530 + Dsui_0521 0.30 +0.4 528,594 + Dsui_0521 0.38 +0.4 528,596 - Dsui_0521 0.39 -0.6 528,599 + Dsui_0521 0.39 -1.9 528,692 + Dsui_0521 0.51 -1.2 528,827 + Dsui_0521 0.68 -0.2 528,846 - Dsui_0521 0.71 -0.6 528,854 + Dsui_0521 0.72 +0.4 528,856 + Dsui_0521 0.72 +0.7 528,940 + Dsui_0521 0.83 +1.5 528,967 - Dsui_0521 0.86 -1.6 528,967 - Dsui_0521 0.86 -0.6 529,045 + -1.9 529,055 + -0.6 529,123 - +0.4 529,200 + -0.6 529,200 + -1.2
Or see this region's nucleotide sequence