Experiment: ALP with Fusidic 0.02 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Dsui_0514 and Dsui_0515 are separated by 131 nucleotides Dsui_0515 and Dsui_0516 are separated by 87 nucleotides Dsui_0516 and Dsui_0517 are separated by 20 nucleotides
Dsui_0514: Dsui_0514 - hypothetical protein, at 518,915 to 520,018
_0514
Dsui_0515: Dsui_0515 - hypothetical protein, at 520,150 to 520,350
_0515
Dsui_0516: Dsui_0516 - Propionyl-CoA carboxylase biotin-containing subunit (EC 6.4.1.3) <small>(from data)</small>, at 520,438 to 522,429
_0516
Dsui_0517: Dsui_0517 - Propionyl-CoA carboxylase carboxyl transferase subunit (EC 6.4.1.3) <small>(from data)</small>, at 522,450 to 523,982
_0517
Position (kb) 520
521
522
523 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 519.472 kb on - strand at 519.513 kb on + strand, within Dsui_0514 at 519.513 kb on + strand, within Dsui_0514 at 519.525 kb on + strand, within Dsui_0514 at 519.539 kb on - strand, within Dsui_0514 at 519.569 kb on - strand, within Dsui_0514 at 519.582 kb on + strand, within Dsui_0514 at 519.642 kb on - strand, within Dsui_0514 at 519.747 kb on + strand, within Dsui_0514 at 519.747 kb on + strand, within Dsui_0514 at 519.815 kb on + strand, within Dsui_0514 at 519.815 kb on + strand, within Dsui_0514 at 519.833 kb on + strand, within Dsui_0514 at 519.833 kb on - strand, within Dsui_0514 at 519.841 kb on - strand, within Dsui_0514 at 519.902 kb on - strand, within Dsui_0514 at 519.984 kb on - strand at 519.991 kb on - strand at 520.163 kb on + strand at 520.238 kb on - strand, within Dsui_0515 at 520.294 kb on - strand, within Dsui_0515 at 520.299 kb on - strand, within Dsui_0515 at 520.360 kb on + strand at 520.508 kb on + strand at 520.531 kb on - strand at 520.551 kb on - strand at 520.575 kb on - strand at 520.651 kb on - strand, within Dsui_0516 at 520.681 kb on + strand, within Dsui_0516 at 520.746 kb on + strand, within Dsui_0516 at 520.801 kb on - strand, within Dsui_0516 at 520.824 kb on - strand, within Dsui_0516 at 520.824 kb on - strand, within Dsui_0516 at 520.852 kb on + strand, within Dsui_0516 at 520.914 kb on - strand, within Dsui_0516 at 520.935 kb on - strand, within Dsui_0516 at 521.008 kb on - strand, within Dsui_0516 at 521.307 kb on - strand, within Dsui_0516 at 521.463 kb on - strand, within Dsui_0516 at 521.513 kb on - strand, within Dsui_0516 at 521.547 kb on + strand, within Dsui_0516 at 521.649 kb on - strand, within Dsui_0516 at 521.649 kb on - strand, within Dsui_0516 at 521.725 kb on - strand, within Dsui_0516 at 521.858 kb on - strand, within Dsui_0516 at 521.870 kb on + strand, within Dsui_0516 at 522.041 kb on + strand, within Dsui_0516 at 522.041 kb on + strand, within Dsui_0516 at 522.059 kb on + strand, within Dsui_0516 at 522.185 kb on + strand, within Dsui_0516 at 522.235 kb on - strand at 522.258 kb on + strand at 522.403 kb on + strand at 522.470 kb on - strand at 522.515 kb on + strand at 522.620 kb on - strand, within Dsui_0517 at 522.682 kb on + strand, within Dsui_0517 at 522.766 kb on - strand, within Dsui_0517 at 522.783 kb on - strand, within Dsui_0517 at 522.829 kb on + strand, within Dsui_0517 at 522.839 kb on + strand, within Dsui_0517 at 523.065 kb on - strand, within Dsui_0517 at 523.075 kb on - strand, within Dsui_0517 at 523.283 kb on - strand, within Dsui_0517
Per-strain Table
Position Strand Gene LocusTag Fraction ALP with Fusidic 0.02 mg/ml remove 519,472 - +0.4 519,513 + Dsui_0514 0.54 -0.7 519,513 + Dsui_0514 0.54 -0.3 519,525 + Dsui_0514 0.55 -0.5 519,539 - Dsui_0514 0.57 -1.6 519,569 - Dsui_0514 0.59 -1.2 519,582 + Dsui_0514 0.60 -0.3 519,642 - Dsui_0514 0.66 -1.6 519,747 + Dsui_0514 0.75 -0.3 519,747 + Dsui_0514 0.75 -0.3 519,815 + Dsui_0514 0.82 -0.3 519,815 + Dsui_0514 0.82 +0.8 519,833 + Dsui_0514 0.83 -0.8 519,833 - Dsui_0514 0.83 -2.4 519,841 - Dsui_0514 0.84 +0.4 519,902 - Dsui_0514 0.89 +0.4 519,984 - -1.6 519,991 - +0.4 520,163 + +0.2 520,238 - Dsui_0515 0.44 -0.6 520,294 - Dsui_0515 0.72 -1.6 520,299 - Dsui_0515 0.74 +0.4 520,360 + -1.6 520,508 + -3.2 520,531 - +1.5 520,551 - +1.3 520,575 - +1.0 520,651 - Dsui_0516 0.11 +0.8 520,681 + Dsui_0516 0.12 -1.9 520,746 + Dsui_0516 0.15 +0.4 520,801 - Dsui_0516 0.18 +0.4 520,824 - Dsui_0516 0.19 +0.7 520,824 - Dsui_0516 0.19 +1.5 520,852 + Dsui_0516 0.21 +0.4 520,914 - Dsui_0516 0.24 +1.3 520,935 - Dsui_0516 0.25 +0.8 521,008 - Dsui_0516 0.29 +0.3 521,307 - Dsui_0516 0.44 +0.4 521,463 - Dsui_0516 0.51 +2.0 521,513 - Dsui_0516 0.54 +0.9 521,547 + Dsui_0516 0.56 +0.4 521,649 - Dsui_0516 0.61 +1.4 521,649 - Dsui_0516 0.61 +1.5 521,725 - Dsui_0516 0.65 +0.9 521,858 - Dsui_0516 0.71 +0.2 521,870 + Dsui_0516 0.72 -1.6 522,041 + Dsui_0516 0.80 -1.2 522,041 + Dsui_0516 0.80 -1.2 522,059 + Dsui_0516 0.81 -1.6 522,185 + Dsui_0516 0.88 +0.4 522,235 - +1.5 522,258 + -1.6 522,403 + -0.6 522,470 - +0.8 522,515 + +0.4 522,620 - Dsui_0517 0.11 +1.2 522,682 + Dsui_0517 0.15 -0.6 522,766 - Dsui_0517 0.21 +0.3 522,783 - Dsui_0517 0.22 +1.2 522,829 + Dsui_0517 0.25 +0.4 522,839 + Dsui_0517 0.25 +0.4 523,065 - Dsui_0517 0.40 +1.5 523,075 - Dsui_0517 0.41 +1.2 523,283 - Dsui_0517 0.54 -0.1
Or see this region's nucleotide sequence