Experiment: ALP with Fusidic 0.02 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Dsui_0501 and Dsui_0502 are separated by 34 nucleotides Dsui_0502 and Dsui_0503 are separated by 46 nucleotides Dsui_0503 and Dsui_0504 overlap by 4 nucleotides Dsui_0504 and Dsui_0505 are separated by 55 nucleotides
Dsui_0501: Dsui_0501 - glycine/D-amino acid oxidase, deaminating, at 503,490 to 504,740
_0501
Dsui_0502: Dsui_0502 - enoyl-CoA hydratase/carnithine racemase, at 504,775 to 505,566
_0502
Dsui_0503: Dsui_0503 - Mg-dependent DNase, at 505,613 to 506,473
_0503
Dsui_0504: Dsui_0504 - putative membrane protein, at 506,470 to 507,375
_0504
Dsui_0505: Dsui_0505 - 7-cyano-7-deazaguanine reductase, at 507,431 to 508,264
_0505
Position (kb) 505
506
507 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 504.780 kb on - strand at 504.780 kb on - strand at 504.808 kb on + strand at 504.828 kb on - strand at 504.830 kb on + strand at 504.945 kb on + strand, within Dsui_0502 at 505.044 kb on + strand, within Dsui_0502 at 505.052 kb on - strand, within Dsui_0502 at 505.060 kb on - strand, within Dsui_0502 at 505.244 kb on + strand, within Dsui_0502 at 505.325 kb on - strand, within Dsui_0502 at 505.340 kb on - strand at 505.407 kb on - strand, within Dsui_0502 at 505.410 kb on + strand, within Dsui_0502 at 505.410 kb on + strand, within Dsui_0502 at 505.618 kb on + strand at 505.713 kb on - strand at 505.713 kb on - strand at 505.810 kb on - strand at 505.810 kb on - strand at 505.875 kb on + strand, within Dsui_0503 at 505.914 kb on + strand, within Dsui_0503 at 505.966 kb on + strand, within Dsui_0503 at 505.989 kb on - strand, within Dsui_0503 at 506.024 kb on - strand, within Dsui_0503 at 506.101 kb on - strand, within Dsui_0503 at 506.134 kb on + strand, within Dsui_0503 at 506.456 kb on - strand at 506.530 kb on - strand at 506.618 kb on - strand at 506.618 kb on - strand, within Dsui_0504 at 506.636 kb on + strand, within Dsui_0504 at 506.644 kb on - strand, within Dsui_0504 at 506.655 kb on + strand, within Dsui_0504 at 506.713 kb on - strand at 506.713 kb on - strand at 506.746 kb on - strand, within Dsui_0504 at 506.826 kb on - strand, within Dsui_0504 at 506.964 kb on + strand, within Dsui_0504 at 506.972 kb on - strand, within Dsui_0504 at 507.005 kb on + strand, within Dsui_0504 at 507.038 kb on + strand, within Dsui_0504 at 507.084 kb on - strand, within Dsui_0504 at 507.150 kb on + strand, within Dsui_0504 at 507.176 kb on - strand, within Dsui_0504 at 507.221 kb on + strand, within Dsui_0504 at 507.254 kb on - strand at 507.351 kb on + strand at 507.352 kb on + strand at 507.357 kb on + strand at 507.365 kb on - strand at 507.369 kb on + strand at 507.369 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction ALP with Fusidic 0.02 mg/ml remove 504,780 - +0.4 504,780 - +0.4 504,808 + +0.5 504,828 - +0.1 504,830 + +0.1 504,945 + Dsui_0502 0.21 +0.1 505,044 + Dsui_0502 0.34 -0.2 505,052 - Dsui_0502 0.35 -1.6 505,060 - Dsui_0502 0.36 -2.6 505,244 + Dsui_0502 0.59 -0.3 505,325 - Dsui_0502 0.69 -3.1 505,340 - -0.6 505,407 - Dsui_0502 0.80 +0.4 505,410 + Dsui_0502 0.80 -0.5 505,410 + Dsui_0502 0.80 +0.4 505,618 + -0.1 505,713 - +0.4 505,713 - +0.4 505,810 - +0.4 505,810 - -1.9 505,875 + Dsui_0503 0.30 +0.5 505,914 + Dsui_0503 0.35 -1.1 505,966 + Dsui_0503 0.41 -0.4 505,989 - Dsui_0503 0.44 -1.2 506,024 - Dsui_0503 0.48 -0.6 506,101 - Dsui_0503 0.57 -2.2 506,134 + Dsui_0503 0.61 -0.5 506,456 - -1.9 506,530 - +0.4 506,618 - +0.4 506,618 - Dsui_0504 0.16 -0.6 506,636 + Dsui_0504 0.18 -2.2 506,644 - Dsui_0504 0.19 +0.4 506,655 + Dsui_0504 0.20 +0.3 506,713 - +0.4 506,713 - +0.4 506,746 - Dsui_0504 0.30 -1.2 506,826 - Dsui_0504 0.39 -1.2 506,964 + Dsui_0504 0.55 -0.1 506,972 - Dsui_0504 0.55 +0.4 507,005 + Dsui_0504 0.59 +1.1 507,038 + Dsui_0504 0.63 +0.2 507,084 - Dsui_0504 0.68 -0.6 507,150 + Dsui_0504 0.75 +0.9 507,176 - Dsui_0504 0.78 -1.2 507,221 + Dsui_0504 0.83 -0.8 507,254 - +0.4 507,351 + -0.4 507,352 + +0.6 507,357 + +0.0 507,365 - +0.4 507,369 + +0.3 507,369 + +1.7
Or see this region's nucleotide sequence