Experiment: ALP with Fusidic 0.02 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Dsui_0472 and Dsui_0473 are separated by 83 nucleotides Dsui_0473 and Dsui_0474 are separated by 83 nucleotides Dsui_0474 and Dsui_0475 are separated by 107 nucleotides
Dsui_0472: Dsui_0472 - nicotinate-nucleotide--dimethylbenzimidazole phosphoribosyltransferase, at 478,938 to 479,993
_0472
Dsui_0473: Dsui_0473 - adenosyl cobinamide kinase/adenosyl cobinamide phosphate guanylyltransferase, at 480,077 to 480,655
_0473
Dsui_0474: Dsui_0474 - universal stress protein UspA-like protein, at 480,739 to 481,191
_0474
Dsui_0475: Dsui_0475 - PilZ domain-containing protein, at 481,299 to 481,670
_0475
Position (kb) 480
481 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 479.080 kb on - strand, within Dsui_0472 at 479.138 kb on - strand, within Dsui_0472 at 479.251 kb on + strand at 479.283 kb on - strand, within Dsui_0472 at 479.335 kb on + strand at 479.343 kb on - strand, within Dsui_0472 at 479.582 kb on + strand, within Dsui_0472 at 479.678 kb on + strand at 479.678 kb on + strand, within Dsui_0472 at 479.752 kb on + strand, within Dsui_0472 at 479.891 kb on + strand at 479.908 kb on + strand at 479.908 kb on + strand at 479.908 kb on + strand at 479.949 kb on + strand at 479.972 kb on + strand at 480.039 kb on + strand at 480.060 kb on + strand at 480.063 kb on - strand at 480.075 kb on - strand at 480.108 kb on - strand at 480.196 kb on - strand, within Dsui_0473 at 480.196 kb on - strand at 480.196 kb on - strand at 480.201 kb on - strand, within Dsui_0473 at 480.260 kb on - strand, within Dsui_0473 at 480.260 kb on - strand, within Dsui_0473 at 480.342 kb on + strand, within Dsui_0473 at 480.409 kb on + strand, within Dsui_0473 at 480.468 kb on - strand, within Dsui_0473 at 480.489 kb on + strand, within Dsui_0473 at 480.500 kb on + strand, within Dsui_0473 at 480.556 kb on - strand, within Dsui_0473 at 480.639 kb on - strand at 480.834 kb on + strand, within Dsui_0474 at 480.888 kb on - strand, within Dsui_0474 at 481.163 kb on + strand at 481.171 kb on - strand at 481.190 kb on - strand at 481.199 kb on + strand at 481.233 kb on + strand at 481.233 kb on + strand at 481.233 kb on + strand at 481.309 kb on - strand at 481.382 kb on + strand at 481.449 kb on - strand, within Dsui_0475 at 481.459 kb on + strand, within Dsui_0475 at 481.477 kb on + strand, within Dsui_0475 at 481.477 kb on + strand, within Dsui_0475 at 481.485 kb on + strand, within Dsui_0475 at 481.611 kb on + strand, within Dsui_0475
Per-strain Table
Position Strand Gene LocusTag Fraction ALP with Fusidic 0.02 mg/ml remove 479,080 - Dsui_0472 0.13 +0.8 479,138 - Dsui_0472 0.19 +0.8 479,251 + +0.4 479,283 - Dsui_0472 0.33 +0.6 479,335 + -0.6 479,343 - Dsui_0472 0.38 -0.2 479,582 + Dsui_0472 0.61 -2.2 479,678 + +0.4 479,678 + Dsui_0472 0.70 -0.6 479,752 + Dsui_0472 0.77 +0.4 479,891 + +0.4 479,908 + -2.9 479,908 + -2.2 479,908 + -0.6 479,949 + -1.6 479,972 + -1.2 480,039 + -1.6 480,060 + +0.4 480,063 - -1.9 480,075 - -0.6 480,108 - -1.2 480,196 - Dsui_0473 0.21 -0.6 480,196 - +0.4 480,196 - +0.4 480,201 - Dsui_0473 0.21 -0.6 480,260 - Dsui_0473 0.32 -2.6 480,260 - Dsui_0473 0.32 -0.6 480,342 + Dsui_0473 0.46 +0.1 480,409 + Dsui_0473 0.57 +0.2 480,468 - Dsui_0473 0.68 -1.2 480,489 + Dsui_0473 0.71 -1.3 480,500 + Dsui_0473 0.73 +0.9 480,556 - Dsui_0473 0.83 -1.9 480,639 - +0.4 480,834 + Dsui_0474 0.21 -0.0 480,888 - Dsui_0474 0.33 -0.6 481,163 + +0.1 481,171 - -1.2 481,190 - -1.9 481,199 + -0.6 481,233 + -0.2 481,233 + -0.2 481,233 + -0.3 481,309 - -0.0 481,382 + -0.6 481,449 - Dsui_0475 0.40 -0.1 481,459 + Dsui_0475 0.43 -2.9 481,477 + Dsui_0475 0.48 -0.6 481,477 + Dsui_0475 0.48 +1.2 481,485 + Dsui_0475 0.50 -2.3 481,611 + Dsui_0475 0.84 -2.2
Or see this region's nucleotide sequence