Experiment: ALP with Fusidic 0.02 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Dsui_0459 and Dsui_0460 are separated by 25 nucleotides Dsui_0460 and Dsui_0461 overlap by 4 nucleotides Dsui_0461 and Dsui_0462 are separated by 83 nucleotides
Dsui_0459: Dsui_0459 - DNA polymerase I, at 462,976 to 465,708
_0459
Dsui_0460: Dsui_0460 - ADP-heptose:LPS heptosyltransferase, at 465,734 to 466,699
_0460
Dsui_0461: Dsui_0461 - glycosyltransferase, at 466,696 to 467,832
_0461
Dsui_0462: Dsui_0462 - ADP-heptose:LPS heptosyltransferase, at 467,916 to 468,938
_0462
Position (kb) 466
467
468 Strain fitness (log2 ratio) -2
-1
0
1
2
3
4 at 465.812 kb on + strand at 465.820 kb on - strand at 465.888 kb on - strand, within Dsui_0460 at 465.940 kb on - strand, within Dsui_0460 at 466.153 kb on + strand at 466.448 kb on + strand, within Dsui_0460 at 466.642 kb on + strand at 466.650 kb on + strand at 466.650 kb on + strand at 466.650 kb on + strand at 466.650 kb on + strand at 466.652 kb on + strand at 466.658 kb on - strand at 466.658 kb on - strand at 466.758 kb on - strand at 466.795 kb on + strand at 466.810 kb on + strand, within Dsui_0461 at 466.880 kb on + strand, within Dsui_0461 at 466.880 kb on + strand, within Dsui_0461 at 466.880 kb on + strand, within Dsui_0461 at 466.903 kb on + strand, within Dsui_0461 at 466.947 kb on + strand, within Dsui_0461 at 467.042 kb on - strand, within Dsui_0461 at 467.107 kb on - strand, within Dsui_0461 at 467.130 kb on - strand, within Dsui_0461 at 467.258 kb on - strand, within Dsui_0461 at 467.447 kb on + strand, within Dsui_0461 at 467.449 kb on + strand, within Dsui_0461 at 467.508 kb on + strand, within Dsui_0461 at 467.532 kb on - strand, within Dsui_0461 at 467.605 kb on + strand, within Dsui_0461 at 467.699 kb on - strand, within Dsui_0461 at 467.759 kb on + strand at 467.777 kb on + strand at 467.785 kb on - strand at 467.785 kb on - strand at 467.787 kb on - strand at 467.852 kb on - strand at 467.890 kb on - strand at 467.890 kb on - strand at 467.896 kb on + strand at 467.953 kb on - strand at 467.975 kb on + strand at 468.018 kb on - strand at 468.026 kb on - strand, within Dsui_0462 at 468.134 kb on - strand, within Dsui_0462 at 468.214 kb on - strand, within Dsui_0462 at 468.257 kb on - strand, within Dsui_0462 at 468.347 kb on - strand, within Dsui_0462 at 468.362 kb on - strand, within Dsui_0462 at 468.363 kb on - strand, within Dsui_0462 at 468.445 kb on + strand, within Dsui_0462 at 468.457 kb on - strand, within Dsui_0462 at 468.527 kb on + strand, within Dsui_0462 at 468.576 kb on + strand, within Dsui_0462 at 468.665 kb on - strand, within Dsui_0462 at 468.709 kb on - strand, within Dsui_0462 at 468.719 kb on + strand, within Dsui_0462 at 468.732 kb on + strand, within Dsui_0462
Per-strain Table
Position Strand Gene LocusTag Fraction ALP with Fusidic 0.02 mg/ml remove 465,812 + +3.3 465,820 - +3.5 465,888 - Dsui_0460 0.16 +3.9 465,940 - Dsui_0460 0.21 +3.7 466,153 + -0.6 466,448 + Dsui_0460 0.74 +0.4 466,642 + -1.9 466,650 + -0.6 466,650 + +0.4 466,650 + +0.4 466,650 + +0.4 466,652 + -2.6 466,658 - +3.5 466,658 - +3.6 466,758 - +1.8 466,795 + -0.6 466,810 + Dsui_0461 0.10 +0.4 466,880 + Dsui_0461 0.16 +0.4 466,880 + Dsui_0461 0.16 +0.4 466,880 + Dsui_0461 0.16 +0.4 466,903 + Dsui_0461 0.18 +0.4 466,947 + Dsui_0461 0.22 -1.6 467,042 - Dsui_0461 0.30 +0.8 467,107 - Dsui_0461 0.36 +1.6 467,130 - Dsui_0461 0.38 +1.3 467,258 - Dsui_0461 0.49 +3.2 467,447 + Dsui_0461 0.66 +1.2 467,449 + Dsui_0461 0.66 +1.5 467,508 + Dsui_0461 0.71 +1.4 467,532 - Dsui_0461 0.74 +1.6 467,605 + Dsui_0461 0.80 +1.1 467,699 - Dsui_0461 0.88 +1.6 467,759 + +1.2 467,777 + +1.1 467,785 - +1.2 467,785 - +0.9 467,787 - +0.5 467,852 - +0.4 467,890 - -0.6 467,890 - -1.2 467,896 + +2.0 467,953 - -2.2 467,975 + +3.2 468,018 - +0.4 468,026 - Dsui_0462 0.11 -1.2 468,134 - Dsui_0462 0.21 -0.6 468,214 - Dsui_0462 0.29 -1.2 468,257 - Dsui_0462 0.33 -1.6 468,347 - Dsui_0462 0.42 -0.6 468,362 - Dsui_0462 0.44 +0.4 468,363 - Dsui_0462 0.44 +0.4 468,445 + Dsui_0462 0.52 +2.7 468,457 - Dsui_0462 0.53 -2.4 468,527 + Dsui_0462 0.60 +2.9 468,576 + Dsui_0462 0.65 +2.6 468,665 - Dsui_0462 0.73 +0.4 468,709 - Dsui_0462 0.78 +1.1 468,719 + Dsui_0462 0.78 +2.9 468,732 + Dsui_0462 0.80 +3.0
Or see this region's nucleotide sequence