Experiment: ALP with Fusidic 0.02 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Dsui_0434 and Dsui_0435 are separated by 64 nucleotides Dsui_0435 and Dsui_0436 are separated by 227 nucleotides
Dsui_0434: Dsui_0434 - ribonuclease, Rne/Rng family, at 440,658 to 442,139
_0434
Dsui_0435: Dsui_0435 - Sel1 repeat protein, at 442,204 to 442,791
_0435
Dsui_0436: Dsui_0436 - dienelactone hydrolase-like enzyme, at 443,019 to 443,903
_0436
Position (kb) 442
443 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1 at 441.408 kb on + strand, within Dsui_0434 at 441.416 kb on - strand at 441.454 kb on - strand at 441.504 kb on + strand, within Dsui_0434 at 441.745 kb on - strand at 441.798 kb on + strand, within Dsui_0434 at 441.882 kb on + strand, within Dsui_0434 at 441.923 kb on - strand, within Dsui_0434 at 442.077 kb on + strand at 442.133 kb on + strand at 442.285 kb on + strand, within Dsui_0435 at 442.346 kb on - strand, within Dsui_0435 at 442.453 kb on - strand, within Dsui_0435 at 442.491 kb on + strand, within Dsui_0435 at 442.572 kb on - strand, within Dsui_0435 at 442.594 kb on + strand, within Dsui_0435 at 442.599 kb on + strand at 442.747 kb on + strand at 442.747 kb on + strand at 442.771 kb on + strand at 442.802 kb on + strand at 442.810 kb on - strand at 442.957 kb on + strand at 443.130 kb on - strand, within Dsui_0436 at 443.135 kb on - strand, within Dsui_0436 at 443.264 kb on + strand at 443.331 kb on + strand, within Dsui_0436 at 443.340 kb on + strand, within Dsui_0436 at 443.401 kb on - strand, within Dsui_0436 at 443.469 kb on + strand, within Dsui_0436 at 443.545 kb on - strand, within Dsui_0436 at 443.729 kb on - strand, within Dsui_0436 at 443.753 kb on + strand, within Dsui_0436 at 443.753 kb on + strand, within Dsui_0436
Per-strain Table
Position Strand Gene LocusTag Fraction ALP with Fusidic 0.02 mg/ml remove 441,408 + Dsui_0434 0.51 -1.9 441,416 - +0.4 441,454 - -0.6 441,504 + Dsui_0434 0.57 -3.4 441,745 - +0.4 441,798 + Dsui_0434 0.77 -4.5 441,882 + Dsui_0434 0.83 -0.6 441,923 - Dsui_0434 0.85 +0.4 442,077 + -1.1 442,133 + +0.2 442,285 + Dsui_0435 0.14 +0.4 442,346 - Dsui_0435 0.24 -0.6 442,453 - Dsui_0435 0.42 -0.6 442,491 + Dsui_0435 0.49 -1.2 442,572 - Dsui_0435 0.63 -0.6 442,594 + Dsui_0435 0.66 +0.4 442,599 + -1.2 442,747 + -1.6 442,747 + +0.4 442,771 + +0.4 442,802 + +0.4 442,810 - -0.6 442,957 + -1.2 443,130 - Dsui_0436 0.13 +0.4 443,135 - Dsui_0436 0.13 -1.2 443,264 + +0.4 443,331 + Dsui_0436 0.35 -1.6 443,340 + Dsui_0436 0.36 -1.6 443,401 - Dsui_0436 0.43 -1.2 443,469 + Dsui_0436 0.51 -1.2 443,545 - Dsui_0436 0.59 -0.6 443,729 - Dsui_0436 0.80 -0.2 443,753 + Dsui_0436 0.83 -3.4 443,753 + Dsui_0436 0.83 +0.4
Or see this region's nucleotide sequence