Experiment: ALP with Fusidic 0.02 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Dsui_0400 and Dsui_0401 are separated by 121 nucleotides Dsui_0401 and Dsui_0402 are separated by 19 nucleotides Dsui_0402 and Dsui_0403 are separated by 82 nucleotides
Dsui_0400: Dsui_0400 - putative nucleoside-diphosphate sugar epimerase, at 397,723 to 399,585
_0400
Dsui_0401: Dsui_0401 - nucleoside-diphosphate-sugar epimerase, at 399,707 to 400,711
_0401
Dsui_0402: Dsui_0402 - glycosyl transferase, at 400,731 to 401,510
_0402
Dsui_0403: Dsui_0403 - lipopolysaccharide heptosyltransferase I, at 401,593 to 402,564
_0403
Position (kb) 399
400
401 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 398.725 kb on + strand, within Dsui_0400 at 398.946 kb on + strand at 398.959 kb on - strand, within Dsui_0400 at 398.985 kb on + strand, within Dsui_0400 at 399.218 kb on - strand, within Dsui_0400 at 399.325 kb on + strand, within Dsui_0400 at 399.333 kb on - strand, within Dsui_0400 at 399.501 kb on + strand at 399.570 kb on + strand at 399.603 kb on - strand at 399.631 kb on + strand at 399.639 kb on - strand at 399.639 kb on - strand at 399.660 kb on + strand at 399.686 kb on - strand at 400.103 kb on + strand at 400.220 kb on - strand, within Dsui_0401 at 400.346 kb on - strand, within Dsui_0401 at 400.492 kb on - strand, within Dsui_0401 at 400.729 kb on + strand at 401.518 kb on + strand at 401.525 kb on - strand at 401.526 kb on - strand at 401.568 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction ALP with Fusidic 0.02 mg/ml remove 398,725 + Dsui_0400 0.54 +0.5 398,946 + +0.5 398,959 - Dsui_0400 0.66 -3.5 398,985 + Dsui_0400 0.68 -2.5 399,218 - Dsui_0400 0.80 -3.0 399,325 + Dsui_0400 0.86 +0.5 399,333 - Dsui_0400 0.86 -3.2 399,501 + -1.1 399,570 + +0.5 399,603 - -3.3 399,631 + +0.5 399,639 - -1.2 399,639 - -0.2 399,660 + -1.5 399,686 - +0.1 400,103 + +0.5 400,220 - Dsui_0401 0.51 -2.0 400,346 - Dsui_0401 0.64 -0.1 400,492 - Dsui_0401 0.78 -2.7 400,729 + +0.5 401,518 + +0.7 401,525 - +0.5 401,526 - +0.5 401,568 + +0.3
Or see this region's nucleotide sequence