Experiment: ALP with Fusidic 0.02 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Dsui_0308 and Dsui_0309 are separated by 2 nucleotides Dsui_0309 and Dsui_0310 are separated by 50 nucleotides Dsui_0310 and Dsui_0311 are separated by 99 nucleotides
Dsui_0308: Dsui_0308 - Major Facilitator Superfamily transporter, at 309,826 to 311,151
_0308
Dsui_0309: Dsui_0309 - DNA/RNA helicase, superfamily II, at 311,154 to 312,485
_0309
Dsui_0310: Dsui_0310 - acyl-CoA hydrolase, at 312,536 to 313,036
_0310
Dsui_0311: Dsui_0311 - ATP-dependent DNA helicase RecG, at 313,136 to 315,211
_0311
Position (kb) 311
312
313 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 310.155 kb on + strand at 310.286 kb on - strand, within Dsui_0308 at 310.292 kb on + strand, within Dsui_0308 at 310.389 kb on + strand at 310.427 kb on + strand at 310.790 kb on - strand, within Dsui_0308 at 310.871 kb on - strand, within Dsui_0308 at 310.932 kb on + strand, within Dsui_0308 at 310.965 kb on + strand at 311.155 kb on - strand at 311.160 kb on + strand at 311.274 kb on + strand at 311.329 kb on - strand, within Dsui_0309 at 311.334 kb on + strand, within Dsui_0309 at 311.335 kb on + strand at 311.365 kb on - strand, within Dsui_0309 at 311.397 kb on + strand at 311.413 kb on - strand, within Dsui_0309 at 311.437 kb on + strand, within Dsui_0309 at 311.525 kb on + strand at 311.569 kb on + strand, within Dsui_0309 at 311.611 kb on + strand, within Dsui_0309 at 311.652 kb on - strand, within Dsui_0309 at 311.698 kb on + strand, within Dsui_0309 at 311.719 kb on + strand, within Dsui_0309 at 311.776 kb on + strand at 311.776 kb on + strand at 311.783 kb on + strand, within Dsui_0309 at 311.861 kb on + strand at 311.896 kb on + strand, within Dsui_0309 at 311.896 kb on + strand at 311.941 kb on + strand at 312.073 kb on - strand, within Dsui_0309 at 312.108 kb on + strand, within Dsui_0309 at 312.108 kb on + strand at 312.115 kb on + strand, within Dsui_0309 at 312.116 kb on - strand, within Dsui_0309 at 312.181 kb on + strand, within Dsui_0309 at 312.394 kb on + strand at 312.488 kb on - strand at 312.488 kb on - strand at 312.491 kb on + strand at 312.750 kb on + strand, within Dsui_0310 at 312.805 kb on + strand, within Dsui_0310 at 312.805 kb on + strand at 312.876 kb on - strand, within Dsui_0310 at 312.917 kb on + strand at 312.925 kb on - strand, within Dsui_0310 at 312.925 kb on - strand, within Dsui_0310 at 312.925 kb on - strand, within Dsui_0310 at 312.925 kb on - strand, within Dsui_0310 at 312.947 kb on - strand, within Dsui_0310 at 313.031 kb on + strand at 313.064 kb on + strand at 313.080 kb on - strand at 313.100 kb on + strand at 313.124 kb on - strand at 313.140 kb on + strand at 313.148 kb on - strand at 313.153 kb on + strand at 313.224 kb on + strand at 313.267 kb on + strand at 313.267 kb on + strand at 313.295 kb on + strand at 313.370 kb on - strand, within Dsui_0311 at 313.412 kb on - strand, within Dsui_0311
Per-strain Table
Position Strand Gene LocusTag Fraction ALP with Fusidic 0.02 mg/ml remove 310,155 + +0.5 310,286 - Dsui_0308 0.35 +1.7 310,292 + Dsui_0308 0.35 -2.1 310,389 + -0.5 310,427 + +0.5 310,790 - Dsui_0308 0.73 -0.5 310,871 - Dsui_0308 0.79 +0.9 310,932 + Dsui_0308 0.83 -2.8 310,965 + -1.5 311,155 - -0.2 311,160 + -0.5 311,274 + -1.1 311,329 - Dsui_0309 0.13 +0.1 311,334 + Dsui_0309 0.14 +0.5 311,335 + -0.5 311,365 - Dsui_0309 0.16 +1.0 311,397 + +0.5 311,413 - Dsui_0309 0.19 +0.5 311,437 + Dsui_0309 0.21 +0.5 311,525 + +0.5 311,569 + Dsui_0309 0.31 -0.5 311,611 + Dsui_0309 0.34 -0.5 311,652 - Dsui_0309 0.37 +0.3 311,698 + Dsui_0309 0.41 -0.5 311,719 + Dsui_0309 0.42 +0.5 311,776 + -1.1 311,776 + +0.5 311,783 + Dsui_0309 0.47 -2.5 311,861 + -0.5 311,896 + Dsui_0309 0.56 +0.5 311,896 + +0.5 311,941 + +0.5 312,073 - Dsui_0309 0.69 +0.6 312,108 + Dsui_0309 0.72 -0.5 312,108 + -0.5 312,115 + Dsui_0309 0.72 -0.5 312,116 - Dsui_0309 0.72 +0.5 312,181 + Dsui_0309 0.77 -1.1 312,394 + -0.5 312,488 - +1.4 312,488 - +0.9 312,491 + -0.5 312,750 + Dsui_0310 0.43 +0.5 312,805 + Dsui_0310 0.54 -0.5 312,805 + -0.5 312,876 - Dsui_0310 0.68 +0.0 312,917 + +0.5 312,925 - Dsui_0310 0.78 +0.4 312,925 - Dsui_0310 0.78 +0.3 312,925 - Dsui_0310 0.78 +1.2 312,925 - Dsui_0310 0.78 +0.9 312,947 - Dsui_0310 0.82 +0.5 313,031 + +0.5 313,064 + -0.5 313,080 - -0.1 313,100 + +0.5 313,124 - +0.5 313,140 + +0.5 313,148 - -1.3 313,153 + +0.5 313,224 + +0.5 313,267 + -2.1 313,267 + +0.5 313,295 + -2.1 313,370 - Dsui_0311 0.11 +0.5 313,412 - Dsui_0311 0.13 +0.7
Or see this region's nucleotide sequence