Experiment: ALP with Fusidic 0.02 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Dsui_0228 and Dsui_0229 are separated by 23 nucleotides Dsui_0229 and Dsui_0230 overlap by 14 nucleotides Dsui_0230 and Dsui_0231 are separated by 19 nucleotides
Dsui_0228: Dsui_0228 - metalloendopeptidase-like membrane protein, at 227,303 to 228,247
_0228
Dsui_0229: Dsui_0229 - Protein of unknown function (DUF721), at 228,271 to 228,711
_0229
Dsui_0230: Dsui_0230 - hypothetical protein, at 228,698 to 228,886
_0230
Dsui_0231: Dsui_0231 - UDP-3-0-acyl N-acetylglucosamine deacetylase, at 228,906 to 229,829
_0231
Position (kb) 228
229 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 227.709 kb on - strand, within Dsui_0228 at 227.864 kb on - strand, within Dsui_0228 at 227.908 kb on + strand, within Dsui_0228 at 227.943 kb on + strand, within Dsui_0228 at 228.046 kb on - strand, within Dsui_0228 at 228.048 kb on - strand, within Dsui_0228 at 228.062 kb on + strand, within Dsui_0228 at 228.067 kb on + strand, within Dsui_0228 at 228.078 kb on + strand, within Dsui_0228 at 228.078 kb on + strand, within Dsui_0228 at 228.091 kb on - strand, within Dsui_0228 at 228.106 kb on + strand, within Dsui_0228 at 228.154 kb on + strand at 228.190 kb on + strand at 228.192 kb on - strand at 228.204 kb on + strand at 228.212 kb on - strand at 228.257 kb on + strand at 228.257 kb on + strand at 228.257 kb on + strand at 228.281 kb on - strand at 228.712 kb on + strand at 228.771 kb on - strand, within Dsui_0230 at 229.871 kb on + strand at 229.871 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction ALP with Fusidic 0.02 mg/ml remove 227,709 - Dsui_0228 0.43 +0.4 227,864 - Dsui_0228 0.59 +0.2 227,908 + Dsui_0228 0.64 -1.2 227,943 + Dsui_0228 0.68 -2.7 228,046 - Dsui_0228 0.79 +0.4 228,048 - Dsui_0228 0.79 +0.1 228,062 + Dsui_0228 0.80 -2.4 228,067 + Dsui_0228 0.81 +0.8 228,078 + Dsui_0228 0.82 -1.8 228,078 + Dsui_0228 0.82 +0.8 228,091 - Dsui_0228 0.83 +0.4 228,106 + Dsui_0228 0.85 -0.2 228,154 + -1.2 228,190 + +0.8 228,192 - +1.8 228,204 + -0.8 228,212 - +0.5 228,257 + +0.6 228,257 + +0.8 228,257 + +0.7 228,281 - +0.6 228,712 + +1.0 228,771 - Dsui_0230 0.39 +0.4 229,871 + -3.5 229,871 + +0.8
Or see this region's nucleotide sequence