Experiment: ALP with Fusidic 0.02 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Dsui_0214 and Dsui_0215 are separated by 2 nucleotides Dsui_0215 and Dsui_0216 overlap by 4 nucleotides Dsui_0216 and Dsui_0217 overlap by 4 nucleotides Dsui_0217 and Dsui_0218 overlap by 62 nucleotides Dsui_0218 and Dsui_0219 are separated by 197 nucleotides
Dsui_0214: Dsui_0214 - TIGR02594 family protein, at 214,590 to 215,081
_0214
Dsui_0215: Dsui_0215 - hypothetical protein, at 215,084 to 215,500
_0215
Dsui_0216: Dsui_0216 - hypothetical protein, at 215,497 to 215,766
_0216
Dsui_0217: Dsui_0217 - hypothetical protein, at 215,763 to 215,936
_0217
Dsui_0218: Dsui_0218 - Integrase, at 215,875 to 217,176
_0218
Dsui_0219: Dsui_0219 - anthranilate synthase component I, at 217,374 to 218,849
_0219
Position (kb) 215
216
217
218 Strain fitness (log2 ratio) -2
-1
0
1 at 214.981 kb on - strand, within Dsui_0214 at 215.216 kb on - strand, within Dsui_0215 at 215.226 kb on - strand, within Dsui_0215 at 215.365 kb on - strand at 215.450 kb on + strand, within Dsui_0215 at 215.482 kb on + strand at 215.494 kb on + strand at 215.495 kb on - strand at 215.626 kb on + strand, within Dsui_0216 at 215.685 kb on + strand at 215.729 kb on + strand, within Dsui_0216 at 215.757 kb on - strand at 215.760 kb on + strand at 215.856 kb on + strand, within Dsui_0217 at 215.886 kb on + strand at 215.894 kb on - strand at 215.965 kb on - strand at 215.988 kb on + strand at 216.049 kb on + strand, within Dsui_0218 at 216.057 kb on - strand, within Dsui_0218 at 216.057 kb on - strand, within Dsui_0218 at 216.121 kb on - strand, within Dsui_0218 at 216.130 kb on - strand, within Dsui_0218 at 216.201 kb on - strand, within Dsui_0218 at 216.300 kb on + strand, within Dsui_0218 at 216.352 kb on - strand, within Dsui_0218 at 216.433 kb on - strand, within Dsui_0218 at 216.443 kb on - strand, within Dsui_0218 at 216.443 kb on - strand, within Dsui_0218 at 216.443 kb on - strand, within Dsui_0218 at 216.685 kb on - strand, within Dsui_0218 at 216.699 kb on + strand, within Dsui_0218 at 216.724 kb on - strand, within Dsui_0218 at 216.785 kb on + strand, within Dsui_0218 at 216.840 kb on + strand at 216.853 kb on - strand, within Dsui_0218 at 216.853 kb on - strand, within Dsui_0218 at 216.858 kb on - strand, within Dsui_0218 at 216.927 kb on + strand, within Dsui_0218 at 217.013 kb on - strand, within Dsui_0218 at 217.037 kb on - strand, within Dsui_0218 at 217.083 kb on + strand at 217.105 kb on + strand at 217.106 kb on + strand at 217.164 kb on + strand at 217.182 kb on + strand at 217.190 kb on - strand at 217.204 kb on + strand at 217.352 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction ALP with Fusidic 0.02 mg/ml remove 214,981 - Dsui_0214 0.79 -1.2 215,216 - Dsui_0215 0.32 +0.8 215,226 - Dsui_0215 0.34 +0.8 215,365 - +0.8 215,450 + Dsui_0215 0.88 -0.2 215,482 + +0.8 215,494 + +0.8 215,495 - -0.2 215,626 + Dsui_0216 0.48 +0.8 215,685 + +0.8 215,729 + Dsui_0216 0.86 -0.2 215,757 - +0.8 215,760 + -1.8 215,856 + Dsui_0217 0.53 -0.8 215,886 + -0.8 215,894 - +0.8 215,965 - -0.7 215,988 + -0.2 216,049 + Dsui_0218 0.13 -2.0 216,057 - Dsui_0218 0.14 -0.3 216,057 - Dsui_0218 0.14 +0.1 216,121 - Dsui_0218 0.19 +0.0 216,130 - Dsui_0218 0.20 +0.1 216,201 - Dsui_0218 0.25 +0.6 216,300 + Dsui_0218 0.33 +0.8 216,352 - Dsui_0218 0.37 +0.9 216,433 - Dsui_0218 0.43 +0.1 216,443 - Dsui_0218 0.44 -0.2 216,443 - Dsui_0218 0.44 +0.3 216,443 - Dsui_0218 0.44 +0.2 216,685 - Dsui_0218 0.62 +0.1 216,699 + Dsui_0218 0.63 +0.1 216,724 - Dsui_0218 0.65 +0.2 216,785 + Dsui_0218 0.70 -0.5 216,840 + +0.8 216,853 - Dsui_0218 0.75 -0.0 216,853 - Dsui_0218 0.75 -0.1 216,858 - Dsui_0218 0.75 +0.9 216,927 + Dsui_0218 0.81 -0.8 217,013 - Dsui_0218 0.87 +0.1 217,037 - Dsui_0218 0.89 +0.5 217,083 + +0.8 217,105 + -1.5 217,106 + -0.2 217,164 + -0.2 217,182 + -0.8 217,190 - +1.1 217,204 + +0.8 217,352 + +0.8
Or see this region's nucleotide sequence