Experiment: ALP with Fusidic 0.02 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Dsui_0123 and Dsui_0124 are separated by 9 nucleotides Dsui_0124 and Dsui_0125 are separated by 4 nucleotides Dsui_0125 and Dsui_0126 are separated by 108 nucleotides
Dsui_0123: Dsui_0123 - hypothetical protein, at 120,968 to 121,624
_0123
Dsui_0124: Dsui_0124 - lactate dehydrogenase-like oxidoreductase, at 121,634 to 122,602
_0124
Dsui_0125: Dsui_0125 - NAD-dependent aldehyde dehydrogenase, at 122,607 to 123,344
_0125
Dsui_0126: Dsui_0126 - (4Fe-4S) cluster-containing protein, at 123,453 to 124,892
_0126
Position (kb)
122
123
124 Strain fitness (log2 ratio)
-2
-1
0
1 at 121.683 kb on + strand at 121.683 kb on + strand at 121.691 kb on - strand at 121.710 kb on + strand at 121.736 kb on + strand at 121.737 kb on + strand at 121.783 kb on + strand, within Dsui_0124 at 121.783 kb on + strand at 121.791 kb on - strand, within Dsui_0124 at 121.842 kb on - strand, within Dsui_0124 at 121.935 kb on - strand, within Dsui_0124 at 121.935 kb on - strand, within Dsui_0124 at 121.997 kb on + strand, within Dsui_0124 at 122.005 kb on - strand, within Dsui_0124 at 122.043 kb on + strand at 122.043 kb on - strand, within Dsui_0124 at 122.065 kb on - strand, within Dsui_0124 at 122.105 kb on + strand, within Dsui_0124 at 122.126 kb on + strand at 122.139 kb on - strand, within Dsui_0124 at 122.155 kb on + strand, within Dsui_0124 at 122.155 kb on + strand at 122.156 kb on + strand at 122.217 kb on - strand, within Dsui_0124 at 122.252 kb on + strand, within Dsui_0124 at 122.281 kb on - strand, within Dsui_0124 at 122.314 kb on - strand, within Dsui_0124 at 122.315 kb on - strand, within Dsui_0124 at 122.341 kb on - strand, within Dsui_0124 at 122.359 kb on - strand, within Dsui_0124 at 122.361 kb on + strand, within Dsui_0124 at 122.449 kb on - strand, within Dsui_0124 at 122.519 kb on - strand at 122.526 kb on + strand at 122.534 kb on - strand at 122.605 kb on + strand at 122.655 kb on - strand at 122.675 kb on - strand at 122.872 kb on - strand, within Dsui_0125 at 122.896 kb on + strand at 123.218 kb on + strand at 123.241 kb on - strand, within Dsui_0125 at 123.272 kb on - strand at 123.282 kb on - strand at 123.287 kb on - strand at 123.315 kb on + strand at 123.317 kb on - strand at 123.331 kb on + strand at 123.360 kb on - strand at 123.395 kb on - strand at 123.427 kb on + strand at 123.446 kb on - strand at 123.451 kb on + strand at 123.459 kb on - strand at 123.493 kb on + strand at 123.655 kb on - strand, within Dsui_0126 at 123.682 kb on - strand, within Dsui_0126 at 123.697 kb on - strand, within Dsui_0126 at 123.770 kb on - strand, within Dsui_0126 at 123.827 kb on - strand, within Dsui_0126 at 123.828 kb on - strand, within Dsui_0126 at 123.839 kb on - strand, within Dsui_0126 at 123.869 kb on - strand, within Dsui_0126 at 123.899 kb on - strand, within Dsui_0126 at 124.007 kb on + strand, within Dsui_0126 at 124.007 kb on + strand at 124.035 kb on - strand, within Dsui_0126 at 124.035 kb on - strand, within Dsui_0126 at 124.092 kb on + strand, within Dsui_0126 at 124.111 kb on + strand at 124.127 kb on + strand, within Dsui_0126 at 124.220 kb on - strand, within Dsui_0126 at 124.276 kb on + strand, within Dsui_0126 at 124.286 kb on - strand at 124.322 kb on - strand, within Dsui_0126 at 124.332 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction ALP with Fusidic 0.02 mg/ml remove 121,683 + +0.8 121,683 + +0.8 121,691 - -0.0 121,710 + -0.2 121,736 + -0.8 121,737 + +0.8 121,783 + Dsui_0124 0.15 -1.3 121,783 + -0.2 121,791 - Dsui_0124 0.16 +0.2 121,842 - Dsui_0124 0.21 +0.7 121,935 - Dsui_0124 0.31 -0.2 121,935 - Dsui_0124 0.31 +0.3 121,997 + Dsui_0124 0.37 -1.2 122,005 - Dsui_0124 0.38 +0.1 122,043 + -0.2 122,043 - Dsui_0124 0.42 +0.6 122,065 - Dsui_0124 0.44 +0.6 122,105 + Dsui_0124 0.49 -1.5 122,126 + +0.8 122,139 - Dsui_0124 0.52 +0.1 122,155 + Dsui_0124 0.54 +0.8 122,155 + +0.8 122,156 + +0.8 122,217 - Dsui_0124 0.60 +0.3 122,252 + Dsui_0124 0.64 -1.2 122,281 - Dsui_0124 0.67 -0.3 122,314 - Dsui_0124 0.70 -0.1 122,315 - Dsui_0124 0.70 +0.7 122,341 - Dsui_0124 0.73 +1.0 122,359 - Dsui_0124 0.75 -0.5 122,361 + Dsui_0124 0.75 -1.2 122,449 - Dsui_0124 0.84 +0.1 122,519 - +0.9 122,526 + +0.8 122,534 - -0.2 122,605 + -0.2 122,655 - -0.1 122,675 - +0.3 122,872 - Dsui_0125 0.36 +0.5 122,896 + +0.8 123,218 + +0.8 123,241 - Dsui_0125 0.86 +0.8 123,272 - +0.7 123,282 - +0.4 123,287 - +1.4 123,315 + +0.8 123,317 - +0.1 123,331 + -2.2 123,360 - +0.4 123,395 - -1.5 123,427 + +0.8 123,446 - +0.8 123,451 + -2.0 123,459 - +0.4 123,493 + -0.8 123,655 - Dsui_0126 0.14 +0.1 123,682 - Dsui_0126 0.16 +0.6 123,697 - Dsui_0126 0.17 +0.9 123,770 - Dsui_0126 0.22 -0.3 123,827 - Dsui_0126 0.26 +1.0 123,828 - Dsui_0126 0.26 +0.1 123,839 - Dsui_0126 0.27 +0.3 123,869 - Dsui_0126 0.29 -0.1 123,899 - Dsui_0126 0.31 +0.2 124,007 + Dsui_0126 0.38 -0.8 124,007 + +0.8 124,035 - Dsui_0126 0.40 +1.3 124,035 - Dsui_0126 0.40 -0.5 124,092 + Dsui_0126 0.44 -2.5 124,111 + +0.8 124,127 + Dsui_0126 0.47 -0.2 124,220 - Dsui_0126 0.53 +1.1 124,276 + Dsui_0126 0.57 -1.2 124,286 - +1.6 124,322 - Dsui_0126 0.60 -0.1 124,332 + -0.2
Or see this region's nucleotide sequence