Experiment: ALP with Fusidic 0.02 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Dsui_0121 and Dsui_0122 are separated by 100 nucleotides Dsui_0122 and Dsui_0123 are separated by 3 nucleotides Dsui_0123 and Dsui_0124 are separated by 9 nucleotides Dsui_0124 and Dsui_0125 are separated by 4 nucleotides
Dsui_0121: Dsui_0121 - glutamyl-tRNA reductase, at 119,122 to 120,393
_0121
Dsui_0122: Dsui_0122 - D-tyrosyl-tRNA(Tyr) deacylase, at 120,494 to 120,964
_0122
Dsui_0123: Dsui_0123 - hypothetical protein, at 120,968 to 121,624
_0123
Dsui_0124: Dsui_0124 - lactate dehydrogenase-like oxidoreductase, at 121,634 to 122,602
_0124
Dsui_0125: Dsui_0125 - NAD-dependent aldehyde dehydrogenase, at 122,607 to 123,344
_0125
Position (kb)
120
121
122 Strain fitness (log2 ratio)
-2
-1
0
1 at 120.429 kb on - strand at 120.447 kb on - strand at 120.517 kb on + strand at 120.714 kb on - strand, within Dsui_0122 at 120.745 kb on + strand at 120.753 kb on - strand, within Dsui_0122 at 120.889 kb on - strand, within Dsui_0122 at 120.907 kb on + strand at 120.974 kb on - strand at 120.974 kb on - strand at 121.029 kb on - strand at 121.036 kb on - strand, within Dsui_0123 at 121.052 kb on + strand, within Dsui_0123 at 121.138 kb on - strand, within Dsui_0123 at 121.149 kb on - strand, within Dsui_0123 at 121.219 kb on + strand, within Dsui_0123 at 121.219 kb on + strand at 121.219 kb on + strand at 121.250 kb on + strand, within Dsui_0123 at 121.265 kb on + strand at 121.399 kb on - strand, within Dsui_0123 at 121.445 kb on - strand, within Dsui_0123 at 121.518 kb on - strand, within Dsui_0123 at 121.683 kb on + strand at 121.683 kb on + strand at 121.691 kb on - strand at 121.710 kb on + strand at 121.736 kb on + strand at 121.737 kb on + strand at 121.783 kb on + strand, within Dsui_0124 at 121.783 kb on + strand at 121.791 kb on - strand, within Dsui_0124 at 121.842 kb on - strand, within Dsui_0124 at 121.935 kb on - strand, within Dsui_0124 at 121.935 kb on - strand, within Dsui_0124 at 121.997 kb on + strand, within Dsui_0124 at 122.005 kb on - strand, within Dsui_0124 at 122.043 kb on + strand at 122.043 kb on - strand, within Dsui_0124 at 122.065 kb on - strand, within Dsui_0124 at 122.105 kb on + strand, within Dsui_0124 at 122.126 kb on + strand at 122.139 kb on - strand, within Dsui_0124 at 122.155 kb on + strand, within Dsui_0124 at 122.155 kb on + strand at 122.156 kb on + strand at 122.217 kb on - strand, within Dsui_0124 at 122.252 kb on + strand, within Dsui_0124 at 122.281 kb on - strand, within Dsui_0124 at 122.314 kb on - strand, within Dsui_0124 at 122.315 kb on - strand, within Dsui_0124 at 122.341 kb on - strand, within Dsui_0124 at 122.359 kb on - strand, within Dsui_0124 at 122.361 kb on + strand, within Dsui_0124 at 122.449 kb on - strand, within Dsui_0124 at 122.519 kb on - strand at 122.526 kb on + strand at 122.534 kb on - strand at 122.605 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction ALP with Fusidic 0.02 mg/ml remove 120,429 - -0.6 120,447 - -1.1 120,517 + -0.2 120,714 - Dsui_0122 0.47 +0.5 120,745 + +0.8 120,753 - Dsui_0122 0.55 +0.0 120,889 - Dsui_0122 0.84 +0.4 120,907 + -0.2 120,974 - +0.4 120,974 - +0.2 121,029 - +0.8 121,036 - Dsui_0123 0.10 +0.1 121,052 + Dsui_0123 0.13 -2.0 121,138 - Dsui_0123 0.26 -0.1 121,149 - Dsui_0123 0.28 +0.9 121,219 + Dsui_0123 0.38 -1.8 121,219 + -0.8 121,219 + -0.8 121,250 + Dsui_0123 0.43 +0.8 121,265 + -0.2 121,399 - Dsui_0123 0.66 +0.9 121,445 - Dsui_0123 0.73 -0.2 121,518 - Dsui_0123 0.84 +0.2 121,683 + +0.8 121,683 + +0.8 121,691 - -0.0 121,710 + -0.2 121,736 + -0.8 121,737 + +0.8 121,783 + Dsui_0124 0.15 -1.3 121,783 + -0.2 121,791 - Dsui_0124 0.16 +0.2 121,842 - Dsui_0124 0.21 +0.7 121,935 - Dsui_0124 0.31 -0.2 121,935 - Dsui_0124 0.31 +0.3 121,997 + Dsui_0124 0.37 -1.2 122,005 - Dsui_0124 0.38 +0.1 122,043 + -0.2 122,043 - Dsui_0124 0.42 +0.6 122,065 - Dsui_0124 0.44 +0.6 122,105 + Dsui_0124 0.49 -1.5 122,126 + +0.8 122,139 - Dsui_0124 0.52 +0.1 122,155 + Dsui_0124 0.54 +0.8 122,155 + +0.8 122,156 + +0.8 122,217 - Dsui_0124 0.60 +0.3 122,252 + Dsui_0124 0.64 -1.2 122,281 - Dsui_0124 0.67 -0.3 122,314 - Dsui_0124 0.70 -0.1 122,315 - Dsui_0124 0.70 +0.7 122,341 - Dsui_0124 0.73 +1.0 122,359 - Dsui_0124 0.75 -0.5 122,361 + Dsui_0124 0.75 -1.2 122,449 - Dsui_0124 0.84 +0.1 122,519 - +0.9 122,526 + +0.8 122,534 - -0.2 122,605 + -0.2
Or see this region's nucleotide sequence